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Analgesic, anti-inflammatory and anti-pyretic activities of D-3-O-methylchiroinositol isolated from stem bark of Piliostigma thonningii

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Abstract

D-3-O-methylchiroinositol isolated from the stem bark of Piliostigma thonningii was evaluated for analgesic, anti-inflammatory and anti-pyretic activities. The compound was screened for analgesic activity by the acetic acid-induced writhing and tail immersion tests. The anti-inflammatory activities were evaluated by the carrageenan-induced paw edema and Freund adjuvant-induced arthritis tests, while its anti-pyretic activity was determined using the yeast-induced pyrexia test. D-3-O-methylchiroinositol (10, 30 and 60 mg/kg) significantly (P < 0.01) reduced the number of acetic acid-induced writhing. In the tail immersion test, by 15, 45, 30 and 60 min post-treatment, tail withdrawal times in D-3-O-methylchiroinositol (30 and 60 mg/kg) were significantly (P < 0.01) longer compared to reaction times recorded in control group. The paw volumes obtained in the 60 mg/kg D-3-O-methylchiroinositol group by 1 h post-carrageenan injection were significantly (P < 0.05) lower than the paw volume of control rats. At 2 and 3 h, the paw volumes in the D-3-O-methylchiroinositol (10, 30 and 60 mg/kg) were significantly (P < 0.05) lower than paw volume of the control group. In the subacute phase of arthritis (18 h), paw volumes in D-3-O-methylchiroinositol (30 and 60 mg/kg) were significantly (P < 0.05 and P < 0.01, respectively) lower compared to paw volume in the control group. In the chronic phase (30 days) of arthritis, paw volumes in the D-3-O-methylchiroinositol (10, 30 and 60 mg/kg) were significantly (P < 0.01) lower than paw volume of the control group. The rectal temperatures of the 60 mg/kg D-3-O-methylchiroinositol were significantly lower at 1, 2, 3, 4 and 5 h post-yeast injection compared to those of the control. The findings of this study establish that D-3-O-methylchiroinositol has analgesic, anti-inflammatory and anti-pyretic activities.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria

    Chinaka O. Nwaehujor

  2. Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria

    Rita I. Udegbunam

  3. Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria

    Isaac U. Asuzu

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  1. Chinaka O. Nwaehujor
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  2. Rita I. Udegbunam
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  3. Isaac U. Asuzu
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Correspondence to Chinaka O. Nwaehujor.

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Nwaehujor, C.O., Udegbunam, R.I. & Asuzu, I.U. Analgesic, anti-inflammatory and anti-pyretic activities of D-3-O-methylchiroinositol isolated from stem bark of Piliostigma thonningii . Med Chem Res 24, 4139–4145 (2015). https://doi.org/10.1007/s00044-015-1459-9

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