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Anticandidiasis agents from a Tanzanian plant, Combretum zeyheri

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Abstract

Combretum zeyheri Sond (Combretaceae) has been reported to exhibit anticandida activity against Candida kruzei, C. albicans, and C. parapsilosis; however, the active constituents have not been isolated so far. A bioactivity-guided fractionation of MeOH extract of C. zeyheri resulted in the isolation of triterpenoids, ursolic acid (1.1), oleanolic acid (1.2), maslinic acid (2.1), 2α,3β-dihydroxyurs-12-en-28-oic acid (2.2), 6β-hydroxymaslinic acid (3), and terminolic acid (4). These compounds were characterized on the basis of their 1D, 2D NMR, ESI-MS and FT-IR spectroscopic data. All the isolated compounds, 1.2, 2.1, 2.2, 3, and 4 except ursolic acid (1.1) are being reported for the first time from C. zeyheri. Later, the isolated triterpenoids (1–4) were evaluated for their anticandida activity against the three strains of C. albicans and all compounds showed anticandida activity of which terminolic acid (4) was most active. Furthermore, structure–activity relationship of isolated triterpenoids (1–4) was studied, which showed that triterpenoids of oleanane and ursane having 2α,3β,23-trihydroxyl group are more active.

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Acknowledgments

Financial support for this research by CSIR and providing TWAS fellowship to one of us (DKBR) is gratefully acknowledged. The authors also thank to all of our colleagues for their excellent assistance.

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Author notes

  1. Deborah K. B. Runyoro

    Present address: School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

Authors and Affiliations

  1. Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015, UP, India

    Deborah K. B. Runyoro & Santosh K. Srivastava

  2. Molecular and Bioprospection Division, Central Institute of Medicinal and Aromatic Plants, Lucknow, UP, India

    Mahendra P. Darokar

  3. School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

    Ngassapa D. Olipa

  4. Chemistry Department, University of Dar es Salaam, Dar es Salaam, Tanzania

    Cosam C. Joseph

  5. School of Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania

    Mecky I. N. Matee

Authors
  1. Deborah K. B. Runyoro
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  2. Santosh K. Srivastava
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  3. Mahendra P. Darokar
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  4. Ngassapa D. Olipa
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Corresponding author

Correspondence to Santosh K. Srivastava.

Electronic supplementary material

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44_2012_135_MOESM1_ESM.doc

Supplementary material 1: The detailed isolation procedures of compounds 1–4, and anticandida activity assay procedures and 1H and 13C-NMR spectroscopic data of 1–4, are available as supporting information (DOC 74 kb).

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Runyoro, D.K.B., Srivastava, S.K., Darokar, M.P. et al. Anticandidiasis agents from a Tanzanian plant, Combretum zeyheri . Med Chem Res 22, 1258–1262 (2013). https://doi.org/10.1007/s00044-012-0135-6

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  • DOI: https://doi.org/10.1007/s00044-012-0135-6

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