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Population genetic structure and colony breeding system in dampwood termites (Zootermopsis angusticollis and Z. nevadensis nuttingi)

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Abstract

Studies describing the population genetic structure and breeding system of basal lineages of termite species remain rare. Such species, however, may reveal ancestral life history attributes potentially influential in the evolution of social life within the Isoptera. Through the development and application of microsatellite DNA loci, we investigated patterns of genetic diversity and differentiation within the dampwood termite Zootermopsis angusticollis collected from three geographically distinct locations in California, USA. Significant genetic differentiation was identified among all sites, which were located 40–150 km apart, and each site was found to represent unique populations with limited levels of gene flow. While Z. angusticollis alates have previously been described as being strong fliers, genetic evidence suggests limited dispersal, possibly due to habitat characteristics restricting long-range flights. Additionally, we characterize patterns of colony genetic structure and breeding system within both Z. angusticollis and its congener Z. nevadensis nuttingi. In Z. angusticollis, simple, extended, and mixed family colonies were observed. The frequency of simple families ranged from 16 to 64%, whereas mixed families were found in only two locations and at low frequencies. In contrast, Z. n. nuttingi, formed primarily extended family colonies. Estimates of relatedness suggest that monogamous pairs heading simple families consist of reproductives showing variable degrees of relatedness from unrelated to close relatives. Additionally, the effective number of neotenic reproductives appears to be low within extended families of both species.

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Acknowledgments

We thank the administrators of the Huddart County Park, the Redwood East Bay Regional Park and the Pebble Beach Company for permission to collect termites. We also thank Tom Juba for valuable assistance in the laboratory. All experiments were conducted in accordance with American statutes governing research. The project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant 2002-35302-12526 to C. Brent. This research was also supported by National Science Foundation Grant IBN-0116857 to J. Traniello and R. Rosengaus.

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Authors and Affiliations

  1. Department of Entomology and W. M. Keck Center for Behavioral Biology, North Carolina State University, Box 7613, Raleigh, NC, 27695, USA

    W. Booth & E. L. Vargo

  2. USDA-ARS, Arid-Land Agricultural Research Center, 21881 North Cardon Lane, Maricopa, AZ, 85138, USA

    C. S. Brent

  3. University of Chicago Laboratory Schools, 1362 E. 59th St., Chicago, IL, 60637, USA

    D. V. Calleri

  4. Department of Biology, Northeastern University, 134 Mugar Life Sciences, 360 Huntington Ave., Boston, MA, 02115, USA

    R. B. Rosengaus

  5. Department of Biology, Boston University, 5 Cummington Street, Boston, MA, 02215, USA

    J. F. A. Traniello

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  1. W. Booth
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Correspondence to W. Booth.

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Booth, W., Brent, C.S., Calleri, D.V. et al. Population genetic structure and colony breeding system in dampwood termites (Zootermopsis angusticollis and Z. nevadensis nuttingi). Insect. Soc. 59, 127–137 (2012). https://doi.org/10.1007/s00040-011-0198-2

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