Abstract
Polyandry in honeybee queens (Apis) causes many patrilines (subfamilies) within a colony, which may lead to a potential conflict of interest among workers. This may be most apparent during queen rearing when nepotistic worker behavior could influence the genetics of future generations. Several studies have searched for such conflict in European honeybees (A. mellifera), but studies on other Apis species remain lacking. We investigated the presence of reproductive conflict in A. cerana japonica by comparing the patriline proportion of queen larvae to that of adult workers. We determined the patrilines of 272 workers and 57 queen larvae using four polymorphic microsatellite markers that were sampled from queenless colonies originally derived from four naturally mated queen-right colonies. The number of patrilines in each colony was 9, 12, 8, and 7, respectively, which is lower than that observed in continental Asia. We found no difference in patriline proportion between adult workers and queen larvae. Our data support neither genetic variance for royalty or existence of worker nepotism in A. cerana japonica.
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Acknowledgment
We thank B. Oldroyd, R. Gill and R. Hammond for their comments on the manuscript. We also thank T. Satoh and N. Kanda for their continuous help for SK.
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Koyama, S., Takagi, T., Martin, S.J. et al. Absence of reproductive conflict during queen rearing in Apis cerana . Insect. Soc. 56, 171–175 (2009). https://doi.org/10.1007/s00040-009-0008-2
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DOI: https://doi.org/10.1007/s00040-009-0008-2