Abstract
Although a large number of recent ecological studies have focused on freshwater bacterioplankton populations, knowledge of the primary factors influencing bacterial communities in urban rivers is limited. Bacterial community structure in three rivers located in Shanghai city was studied over a 1-year period using denaturing gradient gel electrophoresis (DGGE). Subsurface samples were collected every 2 months from two study sites in each river. Water was characterized when biological samples were collected by measuring temperature, pH, chloride ion, total dissolved solids (TDS), total N and total P. complex DGGE band patterns indicated high bacterial diversity in the rivers. Analysis of similarity (ANOSIM) showed that variation of the bacterial communities was higher between rivers than it was between samples from the same river. When evaluated using principal component analysis, samples collected during warmer months from any particular river tended to group together while cold-season samples generally clustered, suggesting significant seasonal impacts. Redundancy analysis was used to identify relationships between environmental factors and bacterial community composition in each individual river. Temperature, TDS, pH, TP and salt concentration were all identified as being related to bacterial community structure, with temperature being the most influential parameter in all three rivers. Our results indicated that bacterial community composition was different in the three different rivers. Even though the rivers were located in heavily disturbed urban ecosystems, temperature was the major driver of bacterial community composition, just as it is in natural systems.
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Araya R, Tani K, Takagi T, Yamaguchi N, Nasu M (2003) Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis. FEMS Microbiol Ecol 43:111–119
Beier S, Witzel KP, Marxsen J (2008) Bacterial community composition in Central European running waters examined by temperature gradient gel electrophoresis and sequence analysis of 16S rRNA genes. Appl Environ Microbiol 74:188–199
Besemer K, Moeseneder MM, Arrieta JM, Herndl GJ, Peduzzi P (2005) Complexity of bacterial communities in a river-floodplain system (Danube, Austria). Appl Environ Microbiol 71:609–620
Buesing N, Filippini M, Burgmann H, Gessner MO (2009) Microbial communities in contrasting freshwater marsh microhabitats. FEMS Microbiol Ecol 69:84–97
Ceballos BS, Soares NE, Moraes MR, Catao RM, Konig A (2003) Microbiological aspects of an urban river used for unrestricted irrigation in the semi-arid region of north-east Brazil. Water Sci Technol 47:51–57
Clarke KR (1993) Non-parametric multivariate analyses of changes in community structure. Aust J Ecol 18:117–143
Clarke KR, Gorley RN (2006) PRIMER v6:user manual/tutorial. PRIMER-E
Clarke KR, Green RH (1988) Statistical design and analysis for a “biological effects” study. Mar Ecol Prog Ser 46:213–226
Clarke KR, Warwick RM (2001) Change in marine communities: an approach to statistical analysis and interpretation. PRIMER-E
Crump BC, Hobbie JE (2005) Synchrony and seasonality in bacterioplankton communities of two temperate rivers. Limnol Oceanogr 50:1718–1729
Curtis TP, Sloan WT, Scannell JW (2002) Estimating prokaryotic diversity and its limits. Proc Natl Acad Sci USA 99:10494–10499
Fazi S, Amalfitano S, Pernthaler J, Puddu A (2005) Bacterial communities associated with benthic organic matter in headwater stream microhabitats. Environ Microbiol 7:1633–1640
Fierer N, Morse JL, Berthrong ST, Bernhardt ES, Jackson RB (2007) Environmental controls on the landscape-scale biogeography of stream bacterial communities. Ecology 88:2162–2173
Hofle MG, Haas H, Dominik K (1999) Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis. Appl Environ Microbiol 65:3164–3174
Hullar MA, Kaplan LA, Stahl DA (2006) Recurring seasonal dynamics of microbial communities in stream habitats. Appl Environ Microbiol 72:713–722
Jarvinen MRM, Ruuhijarvi J, Arvola L (2002) Temporal coherence in water temperature and chemistry under the ice of boreal lakes (Finland). Water Res 36:3949–3956
Jolliffe I (1986) Principal component analysis. Springer, New York
Kent AD, Jones SE, Yannarell AC, Graham JM, Lauster GH, Kratz TK, Triplett EW (2004) Annual patterns in bacterioplankton community variability in a humic lake. Microb Ecol 48:550–560
Kent AD, Yannarell AC, Rusak JA, Triplett EW, McMahon KD (2007) Synchrony in aquatic microbial community dynamics. ISME J 1:38–47
Kirchman DL (1994) The uptake of inorganic nutrients by heterotrophic bacteria. Microb Ecol 28:255–271
Lindstrom ES, Kamst-Van Agterveld MP, Zwart G (2005) Distribution of typical freshwater bacterial groups is associated with pH, temperature, and lake water retention time. Appl Environ Microbiol 71:8201–8206
Liu Y, Yu N, Feng DX, Xiong ZQ, Jiang XQ, Li EC, Chen LQ (2010) Annual variations of zooplankton community structure in Shanghai downtown rivers. Chin J Ecol 29:370–376
Murray K, Fisher L, Therrien J, George B, Gillespie J (2001) Assessment and use of indicator bacteria to determine sources of pollution to an urban river. J Great Lakes Res 27:220–229
Muyzer G, Smalla K (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Leeuwenhoek 73:127–141
Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700
Rao C (1973) Linear statistical inference and its applications, 2nd edn. Wiley, New Yolk
Sanders M, Sivertsen S, Scott G (2002) Origin and distribution of polycyclic aromatic hydrocarbons in surficial sediments from the savannah river. Arch Environ Contam Toxicol 43:438–448
Sekiguchi H, Watanabe M, Nakahara T, Xu B, Uchiyama H (2002) Succession of bacterial community structure along the Changjiang River determined by denaturing gradient gel electrophoresis and clone library analysis. Appl Environ Microbiol 68:5142–5150
Shade A, Chiu CY, McMahon KD (2010) Seasonal and episodic lake mixing stimulate differential planktonic bacterial dynamics. Microb Ecol 59:546–554
Simek K, Armengol J, Comerma M, Garcia JC, Kojecka P, Nedoma J, Hejzlar J (2001) Changes in the epilimnetic bacterial community composition, production, and protist-induced mortality along the longitudinal axis of a highly eutrophic reservoir. Microb Ecol 42:359–371
Stepanauskas R, Moran MA, Bergamaschi BA, Hollibaugh JT (2003) Covariance of bacterioplankton composition and environmental variables in a temperate delta system. Aquat Microb Ecol 31:85–98
Ter Braak CJF, Schaffers AP (2004) Co-correspondence analysis: a new ordination method to relate two community composition. Ecology 85:834–846
ter Braak CJF, Verdonschot PFM (1995) Canonical correspondence analysis and related multivariate methods in aquatic ecology. Aquat Sci 57:255–289
Van der Gucht K, Sabbe K, De Meester L, Vloemans N, Zwart G, Gillis M, Vyverman W (2001) Contrasting bacterioplankton community composition and seasonal dynamics in two neighbouring hypertrophic freshwater lakes. Environ Microbiol 3:680–690
Van der Gucht K, Cottenie K, Muylaert K, Vloemans N, Cousin S, Declerck S, Jeppesen E, Conde-Porcuna JM, Schwenk K, Zwart G, Degans H, Vyverman W, De Meester L (2007) The power of species sorting: local factors drive bacterial community composition over a wide range of spatial scales. Proc Natl Acad Sci USA 104:20404–20409
Van Gremberghe I, Van Wichelen J, Van der Gucht K, Vanormelingen P, D’Hondt S, Boutte C, Wilmotte A, Vyverman W (2008) Covariation between zooplankton community composition and cyanobacterial community dynamics in Lake Blaarmeersen (Belgium). FEMS Microbiol Ecol 63:222–237
Wang CS, Hu XF (2005) Application of ecological technology in the restoration of urban river—case study of Caoyanghuanbang River. Water Resour Dev Res 7:21–23
Winter C, Hein T, Kavka G, Mach RL, Farnleitner AH (2007) Longitudinal changes in the bacterial community composition of the Danube River: a whole-river approach. Appl Environ Microbiol 73:421–431
Yannarell AC, Triplett EW (2004) Within- and between-lake variability in the composition of bacterioplankton communities: investigations using multiple spatial scales. Appl Environ Microbiol 70:214–223
Yannarell AC, Kent AD, Lauster GH, Kratz TK, Triplett EW (2003) Temporal patterns in bacterial communities in three temperate lakes of different trophic status. Microb Ecol 46:391–405
Acknowledgments
This work was supported by grants from Shanghai Water Authority (No. 2007–09), The national Public service sectors (agriculture) research project (No. 200903056) and the Shanghai Education Ministry for the Selection and Cultivation of Young Teachers (79301630).
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Zhang, M., Yu, N., Chen, L. et al. Structure and seasonal dynamics of bacterial communities in three urban rivers in China. Aquat Sci 74, 113–120 (2012). https://doi.org/10.1007/s00027-011-0201-z
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DOI: https://doi.org/10.1007/s00027-011-0201-z