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Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

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Abstract.

Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

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Authors and Affiliations

  1. Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei 11221 (Taiwan), Fax +886 2 28212880, e-mail: losj@ym.edu.tw, , , , , , TW

    E. K.-W. Hui & S. J. Lo

  2. Department of Medicine, School of Medicine, National Yang-Ming University (Taiwan), , , , , , TW

    P.-C. Wang

Authors
  1. E. K.-W. Hui
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  2. P.-C. Wang
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  3. S. J. Lo
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Additional information

Received 9 July 1998; received after revision 2 October 1998; accepted 7 October 1998

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Hui, EW., Wang, PC. & Lo, S. Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants. CMLS, Cell. Mol. Life Sci. 54, 1403–1411 (1998). https://doi.org/10.1007/s000180050262

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  • DOI: https://doi.org/10.1007/s000180050262

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