Abstract
DNA ends can become damaged for various reasons making them unsuitable for TA cloning techniques, the easiest and most common of the DNA cloning technologies. Examples of end-damaged DNA include ancient DNA and those produced by laboratory methods such as sonication. In this chapter, we discuss how to deal with end-damaged DNA prior to cloning with either the popular pGEM®-T Easy Vector Systems Kit and TOPO™ TA Cloning™ Kits.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Ayling, C. (2023). TA Cloning Approaches to Cloning DNA with Damaged Ends DNA. In: Scarlett, G. (eds) DNA Manipulation and Analysis. Methods in Molecular Biology, vol 2633. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3004-4_5
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DOI: https://doi.org/10.1007/978-1-0716-3004-4_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3003-7
Online ISBN: 978-1-0716-3004-4
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