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Curcumin inhibits the pruritus in mice through mast cell MrgprB2 receptor

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Abstract

Background

Curcumin is a diketone compound extracted from the rhizomes of some plants in the Zingiberaceae and Araceae family. It possesses a variety of biological activities, including antioxidant, anti-inflammatory and anti-cancer properties. However, the cellular and molecular antipruritic mechanisms of curcumin remain to be explored.

Objective

Our objective was to study the role of curcumin in pruritus and determine whether its antipruritic effect is related to MrgprB2 receptor.

Methods

The effect of curcumin on pruritus in mice was examined by scratching behavior test. The antipruritic mechanism of curcumin was explored by using transgenic mice (MrgprB2−/− mice, MrgprB2CreTd/tomato mice), histological analysis, western blot and immunofluorescence. In addition, the relationship between curcumin and MrgprB2/X2 receptor was studied in vitro by using calcium imaging, plasmid transfection and molecular docking

Results

In the current study, we found that curcumin had obvious antipruritic effect. Its antipruritic effect was related to the regulation of MrgprB2 receptor activation and mast cells tryptase release. In vitro, mouse peritoneal mast cells activated by compound 48/80 could be inhibited by curcumin. In addition, curcumin was also found to suppress the calcium flux in MrgprX2 or MrgprB2-overexpression HEK cells induced by compound 48/80, substance P, and PAMP 9-20, displaying the specific relation with the MrgprB2/X2 receptor. Moreover, molecular docking results showed that curcumin had affinity to MrgprX2 protein.

Conclusions

Overall, these results indicated that curcumin has the potential to treat pruritus induced by mast cell MrgprB2 receptor.

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Data availability

All data relevant to the study are included in the article or uploaded as supplementary information. Data is available upon request.

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Acknowledgements

The authors thank Prof. Dong in Johns Hopkins for kindly providing the MrgprB2−/−, MrgprB2-Cre and lsl-Tdtomato animals.

Funding

This work was supported by the Project of National Natural Science Foundation of China (No. 31771163) and the key project of science and technology development plan of traditional Chinese medicine in Jiangsu Province (No. ZD202001).

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  1. Yucui Jiang, Yingxin Zong and Ying Du authors contributed equally this paper.

Authors and Affiliations

  1. School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China

    Yucui Jiang, Yingxin Zong, Ying Du, Miaomiao Zhang, Fan Ye, Jian Zhang, Yan Yang, Chan Zhu & Zongxiang Tang

  2. School of Chinese Medicine and School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China

    Yucui Jiang

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ZT: designed and wrote the paper, YJ, YZ and YD: performed experiments, analyzed the data, and wrote part of the paper. All authors read and approved the final manuscript. YJ, YZ and YD: made equal contributions to this work.

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Correspondence to Zongxiang Tang.

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Jiang, Y., Zong, Y., Du, Y. et al. Curcumin inhibits the pruritus in mice through mast cell MrgprB2 receptor. Inflamm. Res. 72, 933–945 (2023). https://doi.org/10.1007/s00011-023-01724-0

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