Abstract
Background
A potential anti-inflammatory effect of clodronate—an aminobisphosphonate—was described to antagonize the pro-inflammatory effects of the block in the mevalonate pathway, the main feature of a rare auto-inflammatory disease called mevalonate kinase deficiency (MKD).
Objective
In this study we evaluated the potential anti-inflammatory effect of clodronate in MKD—a still orphan drug pediatric disease.
Methods
We studied some biological parameters, nitric oxide production using Griess reagents and programmed cell death by flow cytometry, as common inflammatory parameters in MKD, in the presence of different doses of clodronate (1, 10 and 100 μM).
Results
In our cellular model and in monocytes from patients with MKD, clodronate induced an increase in programed cell death and nitric oxide production in comparison with non-treated cells.
Conclusion
Our findings suggest that clodronate does not have an anti-inflammatory effect as previously reported but that it increases the epiphenomena of this pediatric disease.
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Acknowledgments
S.C. is recipient of a fellowship grant from the European Project “Talents for an International House” within the 7th Research & Development Framework Programme PEOPLE–Marie Curie Actions–COFUND (Co-Funding of Regional, National and International Programmes). This work was supported by a grant from the Institute for Maternal and Child Health-IRCCS “Burlo Garofolo”, TS, Italy (RC 42/2011).
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Zanin, V., Marcuzzi, A., Piscianz, E. et al. The effect of clodronate on a mevalonate kinase deficiency cellular model. Inflamm. Res. 61, 1363–1367 (2012). https://doi.org/10.1007/s00011-012-0537-4
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DOI: https://doi.org/10.1007/s00011-012-0537-4