Abstract
Objective and design
In this study, we have investigated the relevance of peripheral blood inflammatory CD14+CD16+ monocytes phenotype to patients with aseptic loosening (AL).
Material and treatment
Immunophenotypes of monocytes were examined among patients with AL (n = 43), patients with mechanical loosening (ML, n = 30), patients with stable implant (SI, n = 16), and patients with osteoarthritis (OA, n = 17) using flow cytometry.
Methods
Immunological assay was used to measure TNF-α and IL-1β levels in both sera and culture media of implant wear stimulated CD14+CD16+ and CD14++CD16− monocytes. Periprosthetic tissues were collected during surgery for histological assessment.
Results
The frequency of CD14+CD16+ monocytes showed significant increase in AL patients than in ML, SI, and OA patients. A positive association was found between the subpopulation of CD14+CD16+ monocytes and plasma TNF-α and IL-1β level in AL patients. Furthermore, a positive correlation existed between the subpopulation of CD14+CD16+ monocytes and the total histopathology score.
Conclusion
The results indicate that CD14+CD16+ monocytes represent a sensitive marker for the disease activity of AL, and may serve as an effective prognostic index to identify total joint replacement recipients who are at increased risk for osteolysis and progression of AL.
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Acknowledgments
This work was supported by Program for Shanghai Key Laboratory of Orthopaedic Implant (08DZ2230330). We would like to thank Dr. John Cuckler, University of Alabama, Birmingham, AL for providing us UHMWPE particles. Thanks also to Dr. Peng Xiaochun for his kind help during patients recruitment and clinical specimen retrieval. And to Prof. Wang Lizhen and Prof. Li Jiang for their kind help in histological assessment of tissue sections.
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Responsible Editor: J. A. Di Battista.
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Wu, W., Zhang, X., Zhang, C. et al. Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening. Inflamm. Res. 58, 561–570 (2009). https://doi.org/10.1007/s00011-009-0020-z
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DOI: https://doi.org/10.1007/s00011-009-0020-z