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Neutrophil haptotaxis induced by mouse MNCF: interactions with extracellular matrix glycoproteins probably contribute to overcoming the anti-inflammatory action of dexamethasone

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Abstract.

Objective and design:

The Macrophage-derived Neutrophil Chemotactic Factor (MNCF) has been characterized as a dexamethasone-resistant neutrophil chemotactic lectin produced by rat macrophages. This study was undertaken to evaluate different MNCF cellular sources and investigate the mechanisms by which MNCF overcomes the anti-inflammatory actions of dexamethasone.

Material and methods:

The mouse macrophage-like cell line P388D1 and thioglycollate-elicited mouse macrophages were studied regarding their capacity to release MNCF. Neutrophil migration assays were performed in vivo and in vitro, in either the presence or absence of extracellular matrix glycoproteins (ECM).

Results:

Mouse and P388D1 macrophages release a lectin that reproduces the activities of rat MNCF. The ability of MNCF to induce neutrophil adhesion and haptotaxis is enhanced through its interaction with laminin and fibronectin. These properties are not inhibited by dexamethasone.

Conclusions:

Together, our results suggest that dexamethasone-resistant neutrophil migration induced by MNCF occurs probably because of its interactions with ECM.

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Authors and Affiliations

  1. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av Bandeirantes 3900, Campus USP, 14049-900, Ribeirão Preto, São Paulo, Brasil

    K. Alves de Toledo, E. Soares Bernardes & M.-C. Roque-Barreira

  2. Departamento de Análises Cínicas, Toxicológicas e Bromatológicas, Faculdade de Ciencias Farmaceuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brasil

    M. Dias Baruffi

Authors
  1. K. Alves de Toledo
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  2. E. Soares Bernardes
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  3. M. Dias Baruffi
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  4. M.-C. Roque-Barreira
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Corresponding author

Correspondence to M.-C. Roque-Barreira.

Additional information

Received 27 September 2006; returned for revision 6 February 2007; accepted by M. Katori 23 April 2007

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de Toledo, K.A., Bernardes, E.S., Baruffi, M.D. et al. Neutrophil haptotaxis induced by mouse MNCF: interactions with extracellular matrix glycoproteins probably contribute to overcoming the anti-inflammatory action of dexamethasone. Inflamm. res. 56, 368–376 (2007). https://doi.org/10.1007/s00011-007-6159-6

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  • DOI: https://doi.org/10.1007/s00011-007-6159-6

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