Skip to main content
SpringerLink
Log in

Adhesion of Listeria monocytogenes to cutting board surfaces and removal by different sanitizers

  • Research Article
  • Published:
Journal für Verbraucherschutz und Lebensmittelsicherheit Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate Listeria monocytogenes adhesion in new and used polyethylene cutting board surfaces and evaluate its removal using different sanitizers (peracetic acid, chlorhexidine, sodium hypochlorite and organic acids). Results showed that the number of adhered cells increased with time on both surfaces evaluated. Statistically significant differences (p < 0.05) between new and used polyethylene surfaces were observed on the adhesion of microorganism. Treatments with the peracetic acid and sodium hypochlorite sanitizers were very effective to inactivate attached L. monocytogenes on the polyethylene cutting board, avoiding the possible formation of biofilms at all contact times studied. Other sanitizers did not succeed in inhibiting completely the pathogen in the maximum concentration (2.0 %) after 24 h of bacterial growth for both surfaces (chlorohexidine), 3 h of bacterial growth on new and 6 h on used surfaces (organic acid). The results indicate that peracetic acid and a sodium hypochlorite treatment present a better method to remove microbial contamination on the surfaces of cutting boards.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Aarnisalo K, Lundén J, Korkeala H, Wirtanen G (2007) Susceptibility of Listeria monocytogenes strains to disinfectants and chlorinated alkaline cleaners at cold temperatures. LWT Food Sci Technol 40:1041–1048. doi:10.1016/j.lwt.2006.07.009

    Article  CAS  Google Scholar 

  • Beltrame CA et al (2013) Comparative lethality kinetic curves and predictive models of F-value for Listeria monocytogenes using different sanitizers. Food Sci Nutr 1:27–31. doi:10.1002/fsn3.5

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Beresford MR, Andrew PW, Shama G (2001) Listeria monocytogenes adheres to many materials found in food-processing environments. J Appl Microbiol 90:1000–1005

    Article  CAS  PubMed  Google Scholar 

  • Bloomfield SF, Scott E (1997) Cross-contamination and infection in the domestic environment and the role of chemical disinfectants. J Appl Microbiol 83:1–9. doi:10.1046/j.1365-2672.1997.00199.x

    Article  CAS  PubMed  Google Scholar 

  • Boks NP, Norde W, van der Mei HC, Busscher HJ (2008) Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces. Microbiology 154:3122–3133. doi:10.1099/mic.0.2008/018622-0

    Article  CAS  PubMed  Google Scholar 

  • Brilhante São José JF, Dantas Vanetti MC (2012) Effect of ultrasound and commercial sanitizers in removing natural contaminants and Salmonella enterica Typhimurium on cherry tomatoes. Food Control 24:95–99. doi:10.1016/j.foodcont.2011.09.008

    Article  Google Scholar 

  • Briñez WJ, Roig-Sagués AX, Hernández Herrero MM, López-Pedemonte T, Guamis B (2006) Bactericidal efficacy of peracetic acid in combination with hydrogen peroxide against pathogenic and non pathogenic strains of Staphylococcus spp., Listeria spp. and Escherichia coli. Food Control 17:516–521. doi:10.1016/j.foodcont.2005.02.014

    Article  Google Scholar 

  • Carpentier B, Cerf O (2011) Review-Persistence of Listeria monocytogenes in food industry equipment and premises. Int J Food Microbiol 145:1–8. doi:10.1016/j.ijfoodmicro.2011.01.005

    Article  PubMed  Google Scholar 

  • Gandhi M, Chikindas ML (2007) Listeria: a foodborne pathogen that knows how to survive. Int J Food Microbiol 113:1–15. doi:10.1016/j.ijfoodmicro.2006.07.008

    Article  PubMed  Google Scholar 

  • Kalmokoff ML, Austin JW, Wan XD, Sanders G, Banerjee S, Farber JM (2001) Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions. J Appl Microbiol 91:725–734

    Article  CAS  PubMed  Google Scholar 

  • Kim H, Bang J, Beuchat LR, Ryu JH (2008) Fate of Enterobacter sakazakii attached to or in biofilms on stainless steel upon exposure to various temperatures or relative humidities. J Food Prot 71:940–945

    PubMed  Google Scholar 

  • Martín-Espada MC, D’Ors A, Bartolomé MC, Pereira M, Sánchez-Fortún S (2014) Peracetic acid disinfectant efficacy against Pseudomonas aeruginosa biofilms on polystyrene surfaces and comparison between methods to measure it. LWT Food Sci Technol 56:58–61. doi:10.1016/j.lwt.2013.11.013

    Article  Google Scholar 

  • Ölmez H, Temur SD (2010) Effects of different sanitizing treatments on biofilms and attachment of Escherichia coli and Listeria monocytogenes on green leaf lettuce. LWT Food Sci Technol 43:964–970. doi:10.1016/j.lwt.2010.02.005

    Article  Google Scholar 

  • Palmer J, Flint S, Brooks J (2007) Bacterial cell attachment, the beginning of a biofilm. J Ind Microbiol Biotechnol 34:577–588. doi:10.1007/s10295-007-0234-4

    Article  CAS  PubMed  Google Scholar 

  • Parizzi SQF (1999) Adesão bacteriana em diferentes superfícies avaliada pela microscopia de epifluorescência e contagem em placas. Dissertation, Federal University of Viçosa

  • Riazi S, Matthews KR (2011) Failure of foodborne pathogens to develop resistance to sanitizers following repeated exposure to common sanitizers. Int Biodeterior Biodegrad 65:374–378. doi:10.1016/j.ibiod.2010.12.001

    Article  CAS  Google Scholar 

  • Rodriguez A, Autio WR, McLandsborough LA (2008) Effect of surface roughness and stainless steel finish on Listeria monocytogenes attachment and biofilm formation. J Food Prot 71:170–175

    PubMed  Google Scholar 

  • Rossoni EMM, Gaylarde CC (2000) Comparison of sodium hypochlorite and peracetic acid as sanitising agents for stainless steel food processing surfaces using epifluorescence microscopy. Int J Food Microbiol 61:81–85. doi:10.1016/S0168-1605(00)00369-X

    Article  CAS  PubMed  Google Scholar 

  • Sinde E, Carballo J (2000) Attachment of Salmonella spp. and Listeria monocytogenes to stainless steel, rubber and polytetrafluorethylene: the influence of free energy and the effect of commercial sanitizers. Food Microbiol 17:439–447. doi:10.1006/fmic.2000.0339

    Article  CAS  Google Scholar 

  • Srey S, Jahid IK, Ha SD (2013) Biofilm formation in food industries: a food safety concern. Food Control 31:572–585. doi:10.1016/j.foodcont.2012.12.001

    Article  Google Scholar 

  • Teixeira P, Lima J, Azeredo J, Oliveira RM (2008) Adhesion of Listeria monocytogenes to materials commonly found in domestic kitchens. Int J Food Sci Technol 43:1239–1244

    Article  CAS  Google Scholar 

  • Van Netten P, Huis in’t Veld JH, Mossel DA (1994) The immediate bactericidal effect of lactic acid on meat-borne pathogens. J Appl Bacteriol 77:490–496

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank CNPq, CAPES, FAPERGS, Science and Technology Secretary RS and URI-Erechim for the financial support for this research.

Author information

Authors and Affiliations

  1. Department of Food Engineering, URI Erechim, Av. Sete de Setembro 1621, Erechim, RS, CEP 99700-000, Brazil

    Cezar A. Beltrame, Eduarda B. Martelo, Raíza A. Mesquita, Juliana Barbosa, Clarice Steffens, Geciane Toniazzo, Eunice Valduga & Rogério L. Cansian

Authors
  1. Cezar A. Beltrame
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eduarda B. Martelo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raíza A. Mesquita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Juliana Barbosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Clarice Steffens
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Geciane Toniazzo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Eunice Valduga
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Rogério L. Cansian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Clarice Steffens.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Beltrame, C.A., Martelo, E.B., Mesquita, R.A. et al. Adhesion of Listeria monocytogenes to cutting board surfaces and removal by different sanitizers. J. Verbr. Lebensm. 10, 41–47 (2015). https://doi.org/10.1007/s00003-014-0923-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00003-014-0923-7

Keywords

Search

Navigation