Abstract
In our earlier study dextran produced byLeuconostoc mesenteroides NRRL B-640 was reported to possess novel food gelling and thickening properties (Puramaet al., 2009). In the present study response surface methodology based experimental designs were applied to enhance the production of this novel dextran byLeuconostoc mesenteroides NRRL B-640. Eleven medium components were examined for their significance on dextran production using Plackett-Burman factorial design. Sucrose, peptone and beef extract were found to have significant effect on the dextran production. The combined effect of these nutrients on dextran production were studied using a 23 full-factorial central composite design, a second-order polynomial was established to identify the relationship between the output i.e. dextran produced and the three medium components. The optimal concentration of variables for maximum dextran production were 5%, w/v sucrose, 2.5%, w/v peptone, and 2.5%, w/v beef extract. The maximum concentration of dextran obtained by predicted model was 12.0 mg/ml that was in perfect agreement with the experimental determined value (12.2±0.2 mg/ml). This value of dextran concentration was over 70 percent higher as compared to un-optimized medium that gave 7.0±0.2 mg/ ml of dextran.
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References
Alsop R.M. (1983). Industrial production of dextrans. In: Bushell M.E., Ed., Progress in Industrial Microbiology, Vol. 18, Elsevier, New York, pp. 1–42.
Barker P.E., Gantesos G., Ajongwen J. (1993). A novel approach to the production of clinical-grade dextran. J. Chem. Technol. Biotechnol., 57: 21–26.
Behravan J., Bazzaz B.S.F., Salimi Z. (2003). Optimization of dextran production byLeuconostoc mesenteroides NRRL B-512F using cheap and local sources of carbohydratre and nitrogen. Biotechnol. Appl. Biochem., 38: 267–269.
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350–356.
Fox J.D., Robyt J.F. (1991). Miniaturization of three carbohydrate analyses using a microsample plate reader. Anal. Biochem., 19: 593–596.
Goyal A., Katiyar S.S. (1996). Regulation of dextransucrase productivityfrom Leuconostoc mesenteroides NRRL B-512F by the maintenance media. J. Gen. Appl. Microbiol., 42: 81–85.
Goyal A., Katiyar, S.S. (1997). Effect of certain nutrients on the production of dextransucrase fromLeuconostoc mesenteroides NRRL B-512F. J. Basic Microbiol., 37: 197–204.
Haider M.A., Pakshirajan K. (2007). Screening and optimization of media constituents for enhancing lipolytic activity by a soil microorganism using statistically designed experiments. Appl. Biochem. Biotechnol., 141: 377–390.
Jeanes A., (1965). Preparation of dextrans from growingLeuconostoc cultures. Methods Carbohydr. Chem., 5: 18–126.
Karthikeyan R.S., Rakshit S.K., Baradarajan A. (1996). Optimization of batch fermentation conditions for dextran production. Bioproc. Eng., 15: 247–251.
Kralj S., Van Geel-Schutten G.H., Dondroff M.G., Kirsanovs S., Van Der Maarel M.J.E.C., Dijkhuizen L. (2004). Glucan synthesis in the genusLactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. Microbiology, 150: 3681–3690.
Lawford G.R., Kligerman A., Williams T., Lawford H.G. (1979). Deaxtran biosynthesis and dextransucrase production by continuous culture ofLeuconostoc mesenteroides. Biotechnol. Bioeng., 21: 1121–1131.
Lazic M.L., Velzkovic V.B., Vucetic J.I., Vrvic M.M., (1993). Effect of pH and aeration on dextran production byLeuconostoc mesenteroides. Enzyme Microb. Technol., 15: 334–338.
Leathers T.D. (2002). Dextran: polysaccharides from prokaryotes. In: Vandamme E.J., De Baets S., Steinbüchel A., Eds, Biopolymers, Polysaccharides?, Wiley-VCH, Weinheim, pp. 299–321.
Li J., Ma C., Ma Y., Li Y. (2007). Medium optimization by combination of response surface methodology and desirability function: an application in glutamine production. Appl. Microbiol. Biotechnol., 74: 563–571.
Liu G.-C., Wang X.-L. (2007). Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production byAgaricus blazei. Appl. Microbiol. Biotechnol., 74: 78–83.
Majumder A., Goyal A. (2008). Enhanced production of exocellular glucansucrase fromLeuconostoc dextranicum NRRL B-1146 using response surface method. Bioresour. Technol., 99: 3685–3691.
Majumder A., Goyal A. (2009) Rheological and gelling properties of a novel glucan fromLeuconostoc dextranicum NRRL B-1146. Food Res. Int., 42: 525–528.
Majumder A., Singh A., Goyal A. (2009). Application of response surface methodology for glucan production fromLeuconostoc dextranicum and its structural characterization. Carbohydr. Polym., 75: 150–156.
Naessens M., Cerdobbel A., Soetaert W., Vandamme E.J. (2005).Leuconostoc dextransucrase and dextran: production, properties and applications. J. Chem. Technol. Biotechnol., 80: 845–860.
Plackett R.L., Burman J.P. (1946). The design of optimum multifactorial experiments. Biometrika, 33: 305–325.
Purama R.K., Goyal A. (2005). Dextransucrase production byLeuconostoc mesenteroides. Ind. J. Microbiol., 2: 89–101.
Purama R.K., Goyal A. 2008. Screening and optimization of nutritional factors for higher dextransucrase production byLeuconostoc mesenteroides NRRL B-640 using statistical approach. Bioresour. Technol., 99: 7108–7114.
Purama R.K., Singh G., Majumder A., Dasu V.V., Goyal A. (2007). Dextransucrase production fromLeuconostoc mesenteroides NRRL B-640 in batch fermentation. Int. J. Chem. Sci., 5: 1497–1504.
Purama R.K., Singh G., Majumder A., Dasu V.V., Goyal A. (2008). Dextransucrase production byLeuconostoc mesenteroides NRRL B-640 in bioreactor: effect of aeration and mathematical modelling. J. Appl. Biosci. Biotechnol., 4: 9–14.
Purama R.K., Goswami P., Khan A.T., Goyal A. (2009). Structural analysis and properties of dextran produced byLeuconostoc mesenteroides NRRL B-640. Carbohydr. Polym., 76, 30–35.
Rodrigues S., Lona L.M.F., Franco T.T. (2003). Effect of phosphate concentration on the production of dextransucrase byLeuconostoc mesenteroides B-512F. Bioproc. Biosyst. Eng., 26: 57–62.
Tsuchiya H.M., Koepsell H.J., Corman J., Bryant G., Bogard M.O., Feger V.H., Jackson R.W. (1952). The effect of certain cultural factors on production of dextransucrase byLeuconostoc mesenteroides. J. Bacteriol., 64: 521–527.
Seymour F.R., Knapp R.D. (1980). Structural analysis of dextrans from strains ofLeuconostoc and related genera that contain 3-O-a-glucosylated-D-glucopyranosyl residues at the branched points, or in consecutive, linear position. Carbohydr. Res., 81: 105–129.
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Majumder, A., Bhandari, S., Purama, R.K. et al. Enhanced production of a novel dextran fromLeuconostoc mesenteroides NRRL B-640 by Response Surface Methodology. Ann. Microbiol. 59, 309–315 (2009). https://doi.org/10.1007/BF03178333
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DOI: https://doi.org/10.1007/BF03178333