Abstract
Tectorigenin and kaikasaponin III from the flowers ofPueraria thunbergiana showed potent hypoglycemic and hypolipidemic effects in the streptozotocin-induced diabetic rats. Intraperitoneal administration of these two compounds with 5 and 10 mg/kg, respectively, for seven days to streptozotocin-induced rats significantly reduced the blood glucose, total cholesterol, LDL- and VLDL-cholesterol and triglyceride levels when compared with those of control group. Glycitein in which 5-OH is unlinked and tectoridin (7-O-glycoside of tectorigenin) isolated from the flowers ofP. thunbergiana did not improve hyperglycemia and hyperlipidemia. In addition, tectorigenin showedin vitro antioxidant effects on 1,1-diphenyl-2-pirylhydrazyl (DPPH) radical, xanthine-xanthine oxidase superoxide anion radical, and lipid peroxidation in rat microsomes induced by enzymatic and non-enzymatic methods. We further found that tectorigenin and kaikasaponin III protected the Vero cell line (normal monkey kidney) from injury by hydrogen peroxide. From these findings, it seems likely that the antioxidant action of tectorigenin and kaikasaponin III may alleviate the streptozotocin-induced toxicity and contribute to hypoglycemic and hypolipidemic effects.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arao, T., Udayama, M., Kinjo, J., Nohara, T., Funakoshi, T. and Kojima, S., Preventive effects of saponins from Puerariae Radix (the root ofPueraria lobata Ohwi) on in vitro immunological injury of rat primary hepatocyte cultures.Biol. Pharm. Bull., 20, 988–991 (1997).
Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem., 72, 248–251 (1976).
Kim, T. J., Korean Resources Plants Vol II., Seoul National University, Seoul, pp. 232–233, 1986.
Kinjo, J., Takeshita, T., Abe, Y., Terada, N., Yamashita, H., Yamasaki, M., Takeuchi, K., Murakami, K., Tomimatsu, T. and Nohara, T., Studies on the constituents ofPueraria lobata. IV. Chemical constituents in the flowers and leaves.Chem. Pharm. Bull., 36, 1174–1179 (1988).
Kiso, Y., Tohkin, M., Hikio, H., Hattori, M., Sakamoto, T. and Namba, Y., Mechanism of antihepatotoxic activity of glycyrrhizin.Planta Medica, 50, 298–302 (1988).
Kubo, M., Sasaki, M., Namba, K., Naruto, S. and Nishimura, H., Isolation of a new isoflavone from Chinese Pueraria flowers.Chem. Pharm. Bull., 23, 2449–2457 (1975).
Kurihara, T. and Kikuchi, M., Studies on the constituents of flowers. I. On the components of flower ofPueraria thunbergiana Benth.Yakugaku Zasshi, 93, 1201–1205 (1973).
Kurihara, T. and Kikuchi, M., Studies on the constituents of flowers. V. On the components of flower ofPueraria thunbergiana Benth. (2). Isolation of a new isoflavone glycoside.Yakugaku Zasshi, 95, 1283–1285 (1975).
Kurihara, T. and Kikuchi, M., Studies on the constituents of flowers. VI. On the components of the flower ofPueraria thunbergiana Benth. (3).Yakugaku Zasshi, 96, 1486–1488 (1976).
McGown, M. W., Artiss, J. D., Strandbergh, D. R. and Zak, B., A peroxidase-coupled method for the colorimetric of serum triglycerides.Clin. Chem., 29, 538–542 (1983).
Miura, T. and Ogiso, T., Lipid peroxidation of erythrocyte membrane induced by xanthine oxidase system: modification of superoxide dismutase effect by hemoglobin.Chem. Pharm. Bull., 30, 3662–3668 (1982).
Miyao, H., Arao, T., Kinjo, J. and Nohara, T., Kaikasaponin III and soyasaponin I, major triterpene saponins ofArbus cantoniensis, act on GOT and GPT: Influence on transaminase elevation of rat liver cells concomitantly exposed to CCl4 for one hour.Plant Medica, 64, 5–7 (1988).
Noma, A., Nezu-Nakayama, K. N., Kota, M. and Okabe, H., Simultaneous determination of serum cholesterol in high- and low-density lipoproteins with use of heparin, Ca++ and an anion exchange resin.Clin. Chem., 24, 1504–1508 (1978).
Noma, A., Okabe, H., Netsu-Nakayama, K. N., Ueno, Y. and Shinobara, H., Improved method for simultaneous determination of cholesterol in high- and low-density lipoprotein.Clin. Chem., 25, 1480–1481 (1979).
Park, H. J., Park, J. H., Moon, J. O., Lee, K. T., Jung, W. T., Oh, S. R. and Lee, H. K., Isoflavone glycoside from the flower ofPueraria thunbergiana.Phytochemistry, 51, 147–151 (1999).
Richmond, W., Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis.Clin. Chem., 22, 1579–1588 (1976).
Sakurai, K. and Ogiso, T., Studies on biological damage by active oxygens. III. Generation of hydroxyl radical and inhibition of insulin release in hypoxanthine-xanthine oxidase system in the presence of pancreatic islet cells.Yakugaku Zasshi, 109, 102–106 (1989).
Sato, T., Kawamoto, A., Tamura, A., Tatsumi, Y. and Fujii, T., Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid).Chem. Pharm. Bull., 40, 721–724 (1992).
Xiong, Q., Kadota, S., Tani, T. and Namba, T., Antioxidative effects of phenylethanoids from Cistanche deserticola.Biol. Pharm. Bull., 19, 1580–1585 (1996).
Yoshikoshi, M., Yoshiki, Y., Okubo, Y., Seto, K. and Sasaki, Y., Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts.Planta Medica, 62, 252–255 (1996).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, K.T., Sohn, I.C., Kim, D.H. et al. Hypoglycemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activityin vitro . Arch Pharm Res 23, 461–466 (2000). https://doi.org/10.1007/BF02976573
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02976573