Abstract
With increasing awareness towards environment-friendly and non-toxic pesticide azadirachtin obtained from neem tree (Azadirachta indica) is gaining more and more importance. Its broad-spectrum activity, peculiar mode of action. eco-friendly and non-toxic action towards beneficial organisms has offered many advantages over chemical pesticides. All currently use commercial formulations based on azadirachtin contains azadirachtin extracted from seeds of naturally grown whole plants which is labour intensive process depending upon many uncontrollable geographical and climatic factors. Plant tissue culture can be a potential process for the production, offering consistent, stable and controlled supply of this bioactive compound, However the research on tissue culture aspects of production are in preliminary stage and requires culture and process optimization for the development of a commercially viable process. This review states the present status and future challenges of plant tissue culture for azadirachtin production.
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References
Jacbson, M. (1988)Focus on Phytochemical Pesticides. Vol 1. The neem tree. CRC Press, Boca Raton, USA.
Immaraju, J. A. (1998) The commercial use of azadirachtin and its integration into viable pest control programmes.Pesti. Sci. 54: 285–289.
Mordue, A. J. and A. Blackwell (1993) Azadirachtin: An update.J. Insect Physiol. 39: 903–924.
Butterworth, J. H. and E. D. Morgan (1968) Isolation of a substance that suppresses feeding in locusts.J. Chem. Soc. Chem. Commun. 46–47.
Govindchari, T. R. (1992) Chemistry and biological investigation onAzadirachta indica (the neem tree).Curr. Sci. 63: 117–122.
Yamaski, K., B. J. A. Klocke, S. M. Lee, G. A. Stone, and M. V. Darlington (1986) Isolation and purification of azadirachtin from neem (Azadirachta indica) seeds using flash chromatography and high-performance chromatography.J. Chromatogr. 356: 220–226.
Govindachari, T. R., G. Sandhya, and S. P. Ganeshraj (1990) Simple method for the isolation of azadirachtin by preparative high-performance liquid chromatography.J. Chromatogr. 513: 389–391.
Sidhu, O. P. and H. M. Behl (1996) Seasonal variation in Azadirachtin in seeds ofAzadirachta indica.Cur. Sci. 70: 12.
Radwanski, S. A. (1977) Neem tree: 1. Commercial potential, characteristics and distribution.World Crops. 29: 62–66.
Koul, O., M. B. Isman, and C. M. Ketkar (1990) Properties and uses of neem,Azadirachta indica.Can. J. Bot. 68: 1–11.
Yakkundi, S. R., R. Thejavathi, and B. Ravindranath (1995) Variation of azadirachtin content during growth and storage of neem (Azadirachta indica) seeds.J. Agri. Food Chem. 43: 2517–2519.
Broughton, H., S. V. Ley, A. M. Z. Slawin, D. J. Williams, and E. D. Morgan (1985). X-ray crystallographic structure determination of detigloyldihydroazadirachtin and reassignment of the structure of the limonoid insect antifeedant Azadirachtin,J. of Chem. Soc. Chem. Commun. 46–47.
Kraus, H., M. Bokel, A. Klank and H. Pohnl (1985) The structure of Azadirachtin and 22,23 Dihydro-23β Methoxyazadirachtin.Tetrahedron letters. 26: 6435–6438.
Ley, S. V., A. A. Denholm, and A. Wood (1993) The chemistry of azadirachtin.Nat. Pro. Rep. 10: 109–157.
Ishihara, J., T. Fukuzaki, and A. Murai (1999) Synthetic studies on Azadirachtin (Part 3): Asymetric syntheis of the tricyclic dihydrofuran moiety of azadirachtin.Tetrahedron. Lett. 40: 1907–1910.
Ishihara, J., Y. Yamamoto, N. Kanoh, and A. Murai (1999) Synthetic studies on Azadirachtin: Construction of the highly fictionalized decalin moiety of azadirachtin.Tetrahedron. Lett. 40: 4387–4390.
Grossman, R. B. and S. V. Ley (1994) Chemistry of insect antifeedants fromAzadirachta indica (part 17): synthesis of model compounds of azadirachtin. Unusual effect of remote substituents on the course of the oxidative ring contraction reactions.Tetrahededron. Lett. 50: 11553–1568.
Alastair, A., A. A. Denholm, L. Jennens, S. V. Ley, and A. Wood (1995) Chemistry of insect antifeedant fromAzadirachta indica (Part 19): A potential relay route for the synthesis of azadirachtin.tetrahedron 51: 6591–6604.
Grossman, R. B. and S. V. Ley (1994) Chemistry of insect antifeedants fromAzadirachta indica (Part 16): Synthesis of several derivatives of azadirachtin containing fluorescent of immunogenic reporter groups.Tetrahedron 50: 8871–8884.
Madyastha, K. and K. Venkatakrishnan (1999) Biocatalyst-mediated expansion of ring D in azadirachtin, a potent insect antifeedant fromAzadirachta indica.Tetrahedron. Lett. 40: 5243–5246.
Schmutterer, H. and K. R. S. Ascher (ed) (1981) Natural pesticides from the neem tree and other tropical plants.Proc. 1st Int. Neem Conf. Rottach-Egern, 1980, GTZ, Eschborn, Germany.
Schmutterer, H. (1995) The Neem Tree, Source of unique natural products for integrated pest management, Medicine. Industry and other purposes. 28. In: H. Schmutterer (ed) VCH verlagsgesellschaft Weinheim, Germany.
Schmutterer, H. and K. R. S. Ascher (1984) Natural pesticides from the neem tree and other tropical plants.Proc. 2nd Int. Neem Conf. Rauischholzhausen, 1983 GTZ, Eschborn, Germany.
Schmutterer, H. and K. R. S. Ascher (1987) Natural pesticides from the neem tree and other tropical plants.Proc. 3rd Int. Neem Conf. Nairobi, 1986 GTZ, Eschborn, Germany.
Schmutterer, H. (1988) Potential of azadirachtin containing pesticides for integrated pest control in developing and industrialized countries.J. Inscet. Physiol. 34: 713–19.
Schmutterer, H. (1990) Properties and potential of natural pesticides from the neem tree,Azadirachta indica.Ann. Rev. Entomol. 35: 271–97.
Warthen, J. D. Jr. (1989) Neem,Azadirachta indica: Organisms affected and reference its update.Proc. ENT. Soc. Wash. 91: 367–388.
Subrahmanyam, B. (1990) Azadirachtin-A naturally occurring insect growth regulator.Proc. Indian Acad. Sci. 99: 277–288.
Ismann, M. B., O. Koul, A. Luczynski, and J. Kaminski (1990) Insecticidal and antifeedant bioactivities of neem oil and their relationship to azadirachtin content.J. Agric. Food. Chem. 38: 1406–1411.
Jones, I. W., A. A. Denholm, S. V. Ley, H. Lovell, A. Wood, and R. E. Sinden (1994) Sexual development of malaria parasite is inhibitedin vitro by the neem extract azadirachtin and its semi-synthetic analogues.FEMS Microbiol. Lett. 120: 367–373.
Rao D. R., R. Reuben, Y. Gitanjali, and G. Srimannarayana (1988) Evaluation of four azadirachtin rich fractions from neem,Azadirachta indica A. Juss (family: Meliaceae) as mosquito laricides.Ind. J. Malaril. 25: 67–72.
Parida, M. M., C. Upadhyay, G. Pandya, and A. M. Jana (2002) Inhibitory potential of neem (Azadirachta indica A. Juss) leaves on Dengue virus type-2 replication.J. Ethnophamacol. 79: 273–278.
Wewetzer, A. (1998) Callus culture ofAzadirachta indica and their potential for the production of azadirachtin.Phytoparasitica. 26: 47–52.
Allan, E. J., J. P. Eeswara, J. Johnson, A. J. Mordue, E. D. Morgan, and T. Stuchbury (1994) The production of azadirachtin byin vitro tissue culture of neem,Azadirachta indica.Pesti. Sci. 42: 147–152.
Kuruvilla, T., P. Komaraiah, and S. V. Ramakrishna (1999) Enhanced secretion of azadirachtin by permeabilized margosa (Azadirachta indica) cells.Ind. J. Exp. Bot. 37: 89–91.
Veeresham, C., M. R. Kumar, | D. Sowjanya, C. K. Kokate, and S. S. Apte (1998) Production of azadirachtin from callus cultures ofAzadirachta indica.Fitoterapia 69: 423–424.
Kearney, M. L., E. J. Allan, J. E. Hooker, and A. J. Mordue (1994) Antifeedant effects of in vitro culture extracts of the neem tree,Azadirachta indica against the desert locust (Schistocerca gregaria).Plant Cell Tiss. Org. Cult. 37: 67–71.
Srividya, N., B. P. Sridevi, and P. Satyanarayana (1998) Azadirachtin and nimbin content inin vitro cultured shoots and roots ofAzadiracta indica A. Juss.Ind. J. Plant Physiol. 3: 129–134.
Chaturvedi, R. (2002)Morphogenesis in Haploid, Diploid and Triploid Tissue Cultures of Azadirachta indica A. Juss. Ph. D Thesis. Department of Botany, Delhi University, Delhi, India.
Venkateshwarlu, B. and K. Mukhopadhyay (1999) Azadirachtin content in the seeds of micropropagated neem plants in relation to its mother tree.Curr. Sci. 76: 626–627.
Zounos, A. K., E. J. Allan, and A. J. Mordue (Luntz) (1999) Bioactive compounds from neem tissue cultures and screening against insects.Pestic. Sci. 55: 486–503.
Ramesh, K. and M. A. Padhya (1980)In vitro propagation of neem (Azadirachta indica A. Juss.) from leaf discs.Ind. J. Exp. Biol. 28: 932–935.
Gautam, V. K., K. Nanda, and S. C. Gupta (1993) Development of shoots and roots in anther-derived callus ofAzadirachta indica A. Juss: A medicinal tree.Plant Cell Tiss. Org. Cult. 34: 13–18.
Thiagarajan, M. and P. M. Murali (1994) Optimum conditions for embryo culture ofAzadirachta indica A. Juss.Ind. Forester. 6: 500–503.
Su, W. W., W. I. Hwang, S. Y. Kin, and Y. Sagwa (1997) Induction of somatic embryogrnesis inAzadirachta indica.Plant Cell Tiss. Org. Cult. 50: 91–95.
Eeswara, J. P., T. Stuchbury, E. J. Allan, and A. J. Mordue (Luntz) (1998) A standard procedure for micropropagation of the neem tree (Azadirachta indica A. Juss.)Plant Cell Rep. 17: 215–219.
Murthy, B. N. S. and P. K. Saxena (1998) Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss.)Plant Cell Rep. 17: 469–475.
Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiol. Plant. 15: 473–497.
White, P. R. (1963)The Cultivation of Animal and Plant Cells. p. 228. The Ronald Press, New York, USA.
Jarvis, A. P., S. Johnson, and E. D. Morgan (1998) Stability of natural insecticide azadirachtin in aqueous and organic solvents.Pestic. Sci. 53: 217–222.
Butterworth, J. H. and E. D. Morgan (1971) Investigation of the locust feeding inhibition of the seeds of the neem tree,Azadirachta indica.J. Insect. Physiol. 17: 969–977.
Turner, C. J., M. S. Tempesta, R. B. Taylor, M. G. Zagorski, J. S. Termini, D. R. Schroeder, and K. Nakanishi (1987) An NMR spectroscopic study of azadirachtin and its trimathyl ether.Tetrahedron 43: 2789–2803.
Schroeder, D. R. and K. Nakanishi (1987) A simplified isolation procedure for azadirachtin.J. Nat. Prod. 50: 241–244.
Sinha, S., P. S. N. Murthy, C. V. N. Rao, G. Ramaprasad, S. Sitaramaiah, D. G. Kumar, and S. K. Savant (1999) Simple method for enrichment of azadirachtin from neem seeds.J. Sci. Ind. Res. 58: 990–994.
Govindchari, T. R. (1995) A direct Preparative high performance liquid chromatography procedure for the isolation of major triterpenoids and their quantative determination in neem oil.J. Liq. Chromatogr. 18: 3456–3471.
Huang, H. P. and E. D. Morgan (1990) Analysis of azadirachtin by supercritical fluid chromatography.J. Chromatogr. 519: 137–143.
Hull, C. J., W. R. Dultton, and B. S. Switzen (1993) Quantification of azadirachtins in insecticidal formulations by high performance liquid chromatography.J. Chromatogr. 633: 330–304.
Thejavathi R, S. R. Yakkundi, and B. Ravidranath (1995) Determination of azadirachtin by reversed-phase high performance liquid chromatography using anisole as internal standard.J. Chromatogr. A. 705: 374–379.
Schutz, S., I. Wengatz, M. H. Goodrow, S. J. Gee, H. E. Hummel, and B. D. Hammock (1997) Development of an enzyme-linked immunosorbent assay for azadirachtins.J. Agri. Food Chem. 45: 2363–2368.
Schaaf, O., A. P. Jarvis, S. A. V. D. Esch, G. Giagnacoco, and N. J. Oldham (2000) Rapid and sensitive analysis of azadirachtin and related triterpenoids from Neem (Azadirachta indica) by high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.J. Chromatogr. 886: 89–97.
Dai, J., V. A. Yaylayan, G. S. Vijayaraghvan, and J. R. Pare (1999) Extraction and colorimetric determination of azadirachtin related limonoids in neem seed kernel.J. Agri. Food Chem. 47: 3738–3742.
Dai, J., V. A. Yaylayan, G. S. Vijayaraghvan, J. R. Pare, and Z. Liu (2001) Multivariate caliberation for the determination of total azadirachtin-related limonoids and simple terpenoids in neem extracts using vanillin assay.J. Agri. Food Chem. 49: 1169–1174.
Jarvis, A. P. and E. D. Morgan (2000) Analysis of small samples of limonoids of neem (Azadirachtaa indica) using solid phase extraction from tissue culture.Phytochem. Anal. 11: 184–189.
Rice, M (1993) Development of neem research and industry in Australia.Proc. World Neem Conf., Australia.
Copping, L. G. and J. J. Menn (2000) Biopesticides: a review of their action, application and efficacy.Pest Manag. Sci. 56: 651–676.
Koul, O., M. B. Isman, and C. M. Ketkar (1990) Properties and uses of neem,Azadirachta indica.Cant. J. Bot. 68: 1–11.
Schmutterer, H. (1995)Insecta, Insects in The Neem Tree. VCH Weinheim, Germany.
Fujita, Y. (1988) Biotechnology in agriculture and forestry. pp. 225–236. In: Y. P. S. Bajaj (ed.)Medicinal and Aromatic Plants. Vol. 4 Springer-Verlag, Berlin, Germany.
Fujita, Y. and Tabata M. (1987)Plant Tissue and Cell Culture. pp. 169–185. Alan R. Liss, New York, USA.
Ushiyama, K. (1991) Large scale culture of ginseng. pp. 92–98. In: A. Komamine, M. Misawa, and F. DiCosmo (eds.)Plant Cell Culture in Japan: Progress in Production of Useful Plant Metabolited by Japanese Enterprises Using Plant Cell Culture Technology, CMC, Japan
Ushiyama, K. and Hibino. K (1997) Commercial production of ginseng by plant cell culture. Am. Chem. Soc. 213 national Meeting, San Francisco, CA, USA.
Ermel, K., E. Pahlich, and H. Schmutterer (1984) Comparison of the azadirachtin content of neem seeds from ecotypes of Asian and African origin.Proc. 2nd Int. Neem Conf. 1983, Rauischholzhausen.
Ermel, K., E. Pahlich, and H. Schmutterer (1987) Azadirachtin content of neem kernels from different geographical locations, and its dependence on temperature, relative humidity and light.Proc. 3 rd Int. Neem Conf. 1986, Nairobi.
Ermel, K. (1995) Azadirachtin content of neem seed kernels from different regions of the world. pp. 222–230. In: H. Schmutterer (ed.).The Neem Tree: Sourse of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. VCH, Weinheim, Germany.
Benge, M. D. (1989) Cultivation and propagation of the neem tree. pp. 1–18. In: M. Jacobson (ed.),Focus on Phytochemical Pesticides. Vol 1, The neem tree. CRC Press, Boca Raton, USA.
Gupta, P. K., Y. C. Tripathi, and M. Rathore (1998) Variation in chemical composition of seeds of neem (Azadirachta indica A. Juss) from different agro-climatic zones of Gujrat.J. Non-timber Forest. 5: 10–13.
Naina, N. S., P. K. Gupta, and A. F. Mascarenhas (1989) Genetic transformation and regeneration of transgenic neem (Azadirachta indica) plants usingAgrobacterium tumefaciens.Curr. Sci. 58: 184–187.
Zhang, Y. H., J. J. Zhong and J. T. Yu (1996) Effect of nitrogen source on cell growth and production of giseng saponin and polysaccharides in suspension culturesPanax notoginseng.Biotechnol. Prog. 12: 567–571.
Bowman, L. and E. Geiger (1984) Optimization of fermentation conditions for alcohol production.Biotechnol. Bioeng. 26: 1492–1497.
Roseiro, J. C., M. E. Esgalhado, M. T. A. Collaco and A. N. Emery (1999) Medium development for xanthan production.Process Biochem 27: 167–175.
Srinivas, M. R. S., N. Chand, and B. K. Lonsane (1994) Use of Placket-Berman design for rapid screening of several nitrogen sources, growth/product promoters, minerals and enzyme inducers for the production of β-galactosidase byAspergillus niger MRSS 234 in solid state fermentation system.Bioprocess Eng. 10: 139–144.
Narang, S., V. Sahai, and V. S. Bisaria (2001) Optimazation of xulanase production by Melanocarpus, albomyces IIS68 in solid-state fermantation using response surface methodology.Biosci Bioeng. 91: 425–427.
Cheyneir, V., M. Feinberg, C. Chararas and C. Ducauze (1983) Application of response surface methodology for evaluation of bioconversion experimental conditions.Appl. Environ. Microbiol. 45: 634–639.
Scragg, A. H. and E. J. Allan, and P. Morris (1988) Investigation into the problems of initiation and maintenance ofCinchona ledgeriana suspension cultures.J. Plant Physiol. 132: 184–189.
Chattopadhyay, S., A. K. Srivastava, S. S. Bhojwani, and V. S. Bisaria (2001) Development of suspension culture ofPodophyllum hexandrum for production of podophyllotoxinBiotechnol. Lett. 23: 2063–2066.
Eilert, U., W. G. W. Kurz, and F. Constable (1985) Stimulation of sanguinarin accumulation inPapaver sominiferum cell cultures by fungal elicitors.J. Plant Physiol. 119: 65–76.
Bohlmann, J., E. Gibraltarskaya, and U. Eilert (1995) Elicitor induction of furanocoumarin biosynthetic pathway in cell cultures ofRuta gravcolens.Plant Cell Tiss. Org. Cult. 43: 155–161.
Furuya, T., T. Yoshikawa, T. Ishii and K. Kajii (1983) Regulation of saponin productionin callus culture ofPanax ginseng.Planta. Med. 47: 200–204.
Linsefors, L., L. Bjork, and K. Mobach (1989) Influence of elicitors and mevalonic acid on the biosynthesis of ginsenosides in tissue culture ofPanax ginseng.Biochem. Physiol. Pflanz. 184: 413–418.
Sundaram, K. M. S. and J. Curry (1996) Effect of some UV light absorber on the photostabilization of azadirachtin, a neem based pesticide.Chemosphere 32: 649–659.
Panda, A. K., S. Mishra, V. S. Bisaria, and S. S. Bhojwani (1989) Plant cell reactors-a perspective.Enzyme Microb. Technol. 11: 386–397.
Doran, P. M. (1995)Bioprocess Engineering Principles, p. 150, Academic Press, London, UK.
Panda, A. K., V. S. Bisaria and S. Mishra (1992) Alkaloid production by plant cell cultures ofHolarrhena antidysentrica: II. Effect of precursor feeding and cultuvation in stirred tank bioreactor.Biotechnol. Bioeng. 39: 1052–1057.
Chattopadhyay, S., A. K. Srivastava, S. S. Bhojwani, and V. S. Bisaria (2002) Production of podophyllotoxin by plant cell culture ofPodophyllum hexandrum in bioreactor.J. Biosci. Bioeng. 93: 215–220.
Zhang, Y. H. and J. J. Zhong (1997) Hyperproduction of ginseng saponinand polysaccarides in high density cultivation ofPanax Notoginseng.Enzyme Microb. Technol. 21: 59–63.
Wu, J. and K. P. Ho (1999) Assessment of various carbon sources and nutrient feeding strategies forPanax ginseng cell culture.Appl. Biochem. Biotechnol. 82: 17–26
Wang, H. Q., J. T. Yu and J. J. Zhong (1999) Significant improvement of taxane production in suspension cultures ofTaxus chinensis by sucrose feeding strategy.Process Biochem. 35: 479–483.
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Prakash, G., Bhojwani, S.S. & Srivastava, A.K. Production of azadirachtin from plant tissue culture: State of the art and future prospects. Biotechnol Bioproc E 7, 185–193 (2002). https://doi.org/10.1007/BF02932968
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DOI: https://doi.org/10.1007/BF02932968