Abstract
Atherosclerosis and hypercholesterolemia have been produced in rabbits since 1913 by feeding them cholesterol. These experiments have a great influence on current thinking about the etiology and possible prevention of ischemic heart disease. Male, New Zealand White rabbits were fed 0.5% dietary cholesterol. Cholesterol and copper in plasma increased sixty-fold and 50%, respectively. Liver copper decreased 74% and hematocrit decreased 25%. Iron was unchanged in heart and liver, but was increased in kidney. Zinc was decreased in heart, but was unchanged in liver or kidney. Changes in organ iron and zinc were smaller than the decrease in liver copper. Similar experiments with higher doses of dietary cholesterol may have resulted in copper deficiency. It may be appropriate to revise interpretations of data from these experiments and to reformulate hypothesis based on the data. Results are consonant with the theoretical implication of copper metabolism and copper deficiency in the etiology and pathogenesis of ischemic heart disease.
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N. Anitschkow and S. Chalatow,Cent. f. Allgemein. Pathol. u. Pathol. Anatom. 24, 1 (1913).
L. Wacker and W. Hueck,Münch. Med. woch. 60, 2097 (1913).
R. Schönheimer,Biochem. Zeit. 147, 258 (1924).
L. M. Klevay,Nutr. Rep. Int. 26, 405–14 (1982).
L. M. Klevay, H. G. Petering, and K. L. Stemmer,Environ. Sci. Techn. 5, 1196–9 (1971).
K. J. Carpenter, A. Gotsis, and D. M. Hegsted,Clin. Chem. 3, 233–8 (1957).
Analytical Methods Committee, Methods for destruction of organic matter,Analyst 85, 643 (1960).
R. A. Jacob, L. M. Klevay, and G. M. Logan, Jr.,Am. J. Clin. Nutr. 31, 477–80 (1978).
B. J. Winer,Statistical Principles in Experimental Design, 2nd ed., McGraw-Hill, New York, 1971, pp. 42, 518–538.
S. Siegel,Nonparametric Statistics for the Behavioral Sciences, McGraw-Hill, NY, 1956, pp. 116–120, 271.
A. B. Hill,Proc. Royal Soc. Med. 58, 295 (1965).
N. P. Singh and D. M. Medeiros,Biol. Trace Elem. Res. 6, 423–29 (1984).
R. L. Kincaid,J. Dairy Sci. 63, 608–610 (1980).
G. W. Evans, N. F. Cornatzer, and W. E. Cornatzer,Am. J. Physiol. 218, 613–615 (1970).
W. H. Parry and S. R. R. Rao, Proceedings of the XII International Congress of Nutrition, San Diego, CA, 1981, p. 38 (abstract #192).
L. M. Klevay,Am. J. Clin. Nutr. 34, 597–598 (1981).
L. M. Klevay,Drug. Nutr. Interact. 2, 131–7 (1983).
L. M. Klevay, Proc. 5th International Symposium on Trace Element Metabolism in Man and Animals, C. F. Mills, I. Bremner, J. K. Chesters, eds., Commonwealth Agricultural Bureaux, Farnham Royal, UK, 1985, pp. 180–183.
L. M. Klevay,Med. Hypotheses 24 111–9 (1987).
L. M. Klevay, inTrace Element Analytical Chemistry in Medicine and Biology, vol. 4, P. Brätter and P. Schramel, eds., de Gruyter, Berlin, 1987, pp. 43–60.
L. M. Klevay,Am. J. Clin. Nutr. 26, 1060–8 (1973).
L. M. Klevay,Am. J. Clin. Nutr. 28, 764–74 (1975).
L. M. Klevay, inAdvances in Nutritional Research, vol. 1, Draper, H. H., ed., Plenum, NY, 1977, pp. 227–52.
L. M. Klevay,Perspect. Biol. Med. 20, 186–92 (1977).
L. M. Klevay,Ann. NY Acad. Sci. 355, 140–51 (1980).
L. M. Klevay,J. Environ. Pathol. Toxicol. 4, 281–7 (1980).
L. M. Klevay, inNutrition and Heart Disease, Naito HK, ed., S. P. Medical & Scientific Books, NY, 1982, pp. 61–7.
L. M. Klevay,Biol. Trace Element Res. 5, 245–55 (1983).
L. M. Klevay, inMetabolism of Trace Metals in Man, vol. 1, Rennert OM, Chan W-Y, CRC Press, Boca Raton, FL, 1984, pp. 129–57.
L. M. Klevay,Clin. Geriatric Med. 3, 361–372 (1987).
H. L. Keil, and V. E. Nelson,J. Biol. Chem. 106, 343–9 (1934).
L. M. Klevay,Nutr. Rep. Int. 22, 617–21 (1980).
L. M. Klevay and K. E. Viestenz,Am. J. Physiol. 240, H185–9 (1981).
L. M. Klevay,Fed. Proc. 45, 235 (1986).
D. M. Medeiros,Nutr. Res. 7, 231–235 (1987).
L. M. Klevay,Nutr. Rep. Int. 35, 999–1006 (1987).
W. Insull, Jr.,Coronary Risk Handbook. American Heart Association, NY, 3,14,15,24,25, 1973.
D. Steinberg,Lancet 2, 205–206 (1985).
K. G. D. Allen and L. M. Klevay,Atherosclerosis 29, 81–93 (1978).
S. J. Kopp, L. M. Klevay, and J. M. Feliksik,Am. J. Physiol. 245, H855–66 (1983).
L. M. Klevay, D. B. Milne, and J. C. Wallwork,Nutr. Rep. Int. 31, 963–71 (1985).
S. Stender, H. Ravn, M. Haugegaard, and K. Kjeldsen,Atherosclerosis 61, 15–23 (1986).
A. C. Beynen, A. G. Lemmens, J. J. DeBruijne, A. Ronai, B. Wassmer, O. Von Deimling, M. B. Katan, and L. F. M. Van Zutphen,Atherosclerosis 63, 239–249 (1987).
G. A. D. Haslewood,Bile Salts, Methuen, London, 1967, pp. 40, 41, 99.
G. A. D. Haslewood,Comprehensive Biochemistry: Sterols, Bile Acids and Steroids, vol. 10, M. Florkin and E. H. Stotz, eds., Elsevier, Amsterdam, 1963, p. 28.
L. M. Klevay,Dietary Fat and Bile Acid Excretion in the Perfused Liver. S. D. in Hygiene Thesis, Harvard University, School of Public Health, Boston, 1966, pp. 44–47, 56–61.
H. Van Belle,Cholesterol, Bile Acids and Atherosclerosis. North-Holland, Amsterdam, 1965, pp. 104–105.
G. W. Evans,Physiol. Rev. 53, 535 (1973).
G. W. Evans,Copper in the Environment: Health Effects, Part II, J. O. Nriagu, ed., Wiley, New York, 1979, p. 163.
J. M. Braganza, R. M. Case, M. H. Jamison and H. Sharma,J. Physiol. 316, 59 (1981).
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Klevay, L.M. Dietary cholesterol lowers liver copper in rabbits. Biol Trace Elem Res 16, 51–57 (1988). https://doi.org/10.1007/BF02795333
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DOI: https://doi.org/10.1007/BF02795333