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Two Hungarian isolates of cucumber mosaic virus from sweet pepper (Capsicum annuum) and melon (Cucumis melo): Identification and antiserum preparation

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Abstract

Two Hungarian virus isolates from sweet pepper (K8) and melon (S4) were identified as cucumber mosaic virus (CMV) on the basis of host plant reactions and serology. The isolates were purified and antisera prepared. Homologous antiserum titers in double-diffusion tests were 256 (K8) and 512 (S4). They were serologically closely related to each other and to other CMV isolates. On the basis of symptoms they belong to different symptomatological groups of CMV; this was supported by serological properties. Sedimentation coefficients were c. 93 S, at 2 mg ml−1. Purified preparations, stained with 2% uranyl acetate, showed spherical particles. In ELISA purified preparations reacted with each other's antisera.

Samenvatting

Twee hongaarse virusisolaten uit paprika (K8) en meloen (S4) werden geïdentificeerd als komkommermozaïekvirus (CMV) met behulp van toetsplanten en serologie. Beide isolaten werden gezuiverd en er werden antisera tegen bereid. De homologe titers van de antisera in de agar-geldiffusietoets bedroegen 256 (K8) en 512 (S4). K8 en S4 waren serologisch nauw verwant aan elkaar, evenals aan andere CMV-isolaten. Op grond van hun symptomen op toetsplanten behoren ze tot verschillende symptomatologische groepen van CMV. Dit laatste werd gesteund door de serologische eigenschappen. Beide isolaten hebben een sedimentatiecoëfficiënt van ca 93 S, bij een concentratie van 2 mg ml−1. Gezuiverde preparaten, gekleurd met 2% uranylacetaat, bleken bolvormige deeltjes te bevatten. In ELISA reageerden gezuiverde preparaten van K8 en S4 met elkaars antisera.

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Author notes

  1. D. Z. Maat & H. Huttinga

    Present address: Research Institute for Plant Protection (IPO), Wageningen

  2. I. Tóbiás (Guest worker from September 1981 till January 1982 as a fellow of the International Agricultural Centre)

    Present address: Vegetable Crops Research Institute, Park u. 2, 1223, Budapest, Hungary

Authors and Affiliations

  1. Research Institute for Plant Protection (IPO), Wageningen

    I. Tóbiás (Guest worker from September 1981 till January 1982 as a fellow of the International Agricultural Centre)

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  1. I. Tóbiás
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  2. D. Z. Maat
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  3. H. Huttinga
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Tóbiás, I., Maat, D.Z. & Huttinga, H. Two Hungarian isolates of cucumber mosaic virus from sweet pepper (Capsicum annuum) and melon (Cucumis melo): Identification and antiserum preparation. Netherlands Journal of Plant Pathology 88, 171–183 (1982). https://doi.org/10.1007/BF02140880

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