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Carbonic anhydrase-III immunohistochemical localization in human skeletal muscle

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Summary

Using the indirect immunoperoxidase method, we studied the localization of carbonic anhydrase-III (CA-III) in frozen sections of biopsies of human skeletal muscle which had no definite pathology. CA-III was found to be localized in Type-I muscle fibers when compared with serial sections stained with myosin ATPase and other reactions. Our finding was in accordance with the biochemical data so far reported. It was thought that CA-III could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases.

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References

  • Carter N, Jeffery S, Shiels A, Edwards Y, Tipler T, Hopkinson DA (1979) Characterization of human carbonic anhydrase III from skeletal muscle. Biochem Genet 17:837–854

    Google Scholar 

  • Heath R, Jeffery S, Carter N (1982) Radioimmunoassay of human muscle carbonic anhydrase III in dystrophic states. Clin Chim Acta 119:299–305

    Google Scholar 

  • Lönnerholm G (1980) Carbonic anhydrase in rat liver and rabbit skeletal muscle; Further evidence for the specificity of the histochemical cobalt-phosphate method of Hansson. J Histochem Cytochem 28:427–433

    Google Scholar 

  • Moynihan JB (1977) Carbonic anhydrase activity in mammalian skeletal and cardiac muscle. Biochem J 168:567–569

    Google Scholar 

  • Nishita T, Deutsch HF (1981) Isolation of equine muscle carbonic anhydrase in crystalline form. Biochem Biophys Res Commun 103:573–580

    Google Scholar 

  • Ridderstråle Y (1979) Observation on the localization of carbonic anhydrase in muscle. Acta Physiol Scand 106:239–240

    Google Scholar 

  • Zborowska-Sluis DT, L'Abbate A, Klassen GA (1974) Evidence of carbonic anhydrase activity in skeletal muscle: A role for facilitative carbon dioxide transport. Resp Physiol 21:341–350

    Google Scholar 

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Authors and Affiliations

  1. Section of Neurology, Dept. of Neurosurgery, Hokkaido University School of Medicine, 060, Sapporo, Japan

    K. Shima & K. Tashiro

  2. First Dept. of Biochemistry, Hokkaido University School of Medicine, 060, Sapporo, Japan

    K. Shima, N. Hibi, Y. Tsukada & H. Hirai

Authors
  1. K. Shima
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  2. K. Tashiro
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  3. N. Hibi
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  4. Y. Tsukada
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  5. H. Hirai
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Shima, K., Tashiro, K., Hibi, N. et al. Carbonic anhydrase-III immunohistochemical localization in human skeletal muscle. Acta Neuropathol 59, 237–239 (1983). https://doi.org/10.1007/BF00703210

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  • DOI: https://doi.org/10.1007/BF00703210

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