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The ontogenetic development of serotonin (5-HT1) receptors in various cortical regions of the rat brain

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Summary

The distribution of serotonin (5-HT1) receptors in various cortical regions of the rat brain has been examined during ontogenesis by quantitative autoradiography.

An increase in binding site density between the first postnatal day and adult age was observed and could be approximated by a sigmoid shaped (logistic) growth curve. A marked heterochrony in the increase of binding site density is found in the 13 analyzed cortical regions. Binding sites develop earlier in neocortex than in allocortical areas. Fifty pereent of the binding site density of adult age is reached in the motor cortex at the 9th postnatal day, followed by the primary somatosensory cortex one day later, by the medial prefrontal cortex on the 12th day, by the fascia dentata on the 14th day and by the CA1-region on the 20th day. A detailed analysis of the frontal, medial prefrontal and hippocampal regions also shows a heterochrony within these regions. Adult values of binding site densities are also reached at different ages in the various cortical regions. The highest receptor densities were observed in the dorsal subiculum, the lowest in the primary somatosensory cortex.

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References

  • Alexander GM, Schwartzman RJ (1984) Quantitative computer analysis of autoradiographs utilizing a charge-coupled device solid-state camera. J Neurosci Methods 12:29–36

    Google Scholar 

  • Altar CA, Walter RJ, Neve KA, Marshall JF (1984) Computerassisted video analysis of [3H]spiroperidol binding autoradiographs. J Neurosci Methods 10:173–188

    Google Scholar 

  • Baumgarten HG, Lachenmeyer L (1972) 5,7-Dihydroxytryptamine: Improvement in chemical lesioning of indolamine neurons in the mammalian brain. Z Zellforsch 135:399–414

    Google Scholar 

  • Beaudet A, Descarries L (1976) Quantitative data on serotonin nerve terminals in adult rat cortex. Brain Res 111:301–309

    Google Scholar 

  • Beaudet A, Descarries L (1979) Radioautographic characterization of a serotonin-accumulating nerve cell group in adult rat hypothalamus. Brain Res 160:231–243

    Google Scholar 

  • Biegon A, Rainbow TC, McEwen BS (1982) Quantitative autoradiography of serotonin receptors in the rat brain. Brain Res 242:197–204

    Google Scholar 

  • Chan-Palay V (1975) Fine structure of labeled axons in the cerebellar cortex and nuclei of rodents and primates after intraventricular infusions with tritiated serotonin. Anat Embryol 148:235–265

    Google Scholar 

  • Chan-Palay V (1982) Serotonin neurons and their axons in the raphe dorsalis of rat and rhesus monkey: Demonstration by high resolution autoradiography with 3H-serotonin. In: Chan-Palay V, Palay SL (eds) Cytochemical methods in neuroanatomy, Alan R Liss, New York

    Google Scholar 

  • Chan-Palay V, Jonsson G, Palay SL (1978) Serotonin and substance P coexist in neurons of the rat's central nervous system. Proc Natl Acad Sci USA 75:1582–1586

    Google Scholar 

  • Dahlström A, Fuxe K (1964) Evidence for the existence of monoamine-containing neurons in the central nervous system. Acta Physiol Scand 62 (Suppl) 232:1–55

    Google Scholar 

  • Descarries L, Beaudet A, Watkins KC (1978) Serotonin nerve terminals in adult rat neocortex. Brain Res 100:563–588

    Google Scholar 

  • Desmukh PP, Yamamura HI, Woods L, Nelson DL (1983) Computer-assisted autoradiographic localization of subtypes of serotonin receptors in rat brain. Brain Res 288:338–343

    Google Scholar 

  • Fuxe K (1965) Evidence for the existence of monoamine neurons in the central nervous system. V. Distribution of monoamine terminals in the central nervous system. Acta Physiol Scand 64:37–38

    Google Scholar 

  • Fuxe K, Jonsson G (1967) A modification of the histochemical fluorescence method for the improved localization of 5-hydroxytryptamine. Histochemie 11:161–166

    Google Scholar 

  • Glaser T, Traber J (1983) Buspirone: action on serotonin receptors in calf hippocampus. Eur J Pharmacol 88:137–138

    Google Scholar 

  • Glaser T, Rath M, Traber J, Zilles K, Schleicher A (in press) Autoradiographic identification and topographical analyses of high affinity serotonin receptor subtypes as a target for the novel putative anxiolytic TVX Q7821. Brain Res

  • Hökfelt T, Ljungdahl A, Steinbusch H, Verhofstadt A, Nilsson G, Brodin E, Pernow B, Goldstein M (1978) Immunohistochemical evidence of substance P-like immunoreactivity in some 5-hydroxytryptamine-containing neurons in the rat central nervous system. Neuroscience 3:517–538

    Google Scholar 

  • Köhler C (1984) The distribution of serotonin binding sites in the hippocampal region of the rat brain. An autoradiographic study. Neuroscience 13:667–680

    Google Scholar 

  • Köhler C, Ross SB, Srebro B, Ögren SO (1978) Long-term biochemical and behavioral effects of p-chloroamphetamine in the rat. Ann NY Acad Sci 305:645–663

    Google Scholar 

  • Kretschmann HJ, Wingert F (1971) Computeranwendungen bei Wachstumsproblemen in Biologie und Medizin. Springer, Berlin, Heidelberg, New York

    Google Scholar 

  • Kuhar MJ, Unnerstall JR (1985) Quantitative receptor mapping by autoradiography: some current technical problems. TINS 8:49–53

    Google Scholar 

  • Lamour Y, Privot JP, Pointis D, Ory-Lavollee L (1983) Laminar distribution of serotonergic innervation in rat somatosensory cortex, as determined by in vivo electrochemical detection. Brain Res 259:163–166

    Google Scholar 

  • Lidov HGW, Grzanna R, Molliver ME (1980) The serotonin innervation of the cerebral cortex in the rat — An immunohistochemical analysis. Neuroscience 5:207–227

    Google Scholar 

  • Marcinkiewicz M, Vergé D, Gozlan H, Pichat L, Hamon M (1984) Autoradiographic evidence for the heterogeneity of 5-HT1 sites in the brain. Brain Res 291:159–163

    Google Scholar 

  • Mares V, Schultze B, Maurer W (1974) Stability of DNA in Purkinje cell nuclei of the mouse. An autoradiographic study. J Cell Biol 63:665–674

    Google Scholar 

  • Meibach RC, Maayani S, Green JP (1980) Characterization and radioautography of [3H]LSD binding by rat brain slices in vitro: The effect of 5-hydroxytryptamine. Eur J Pharmacol 67:371–382

    Google Scholar 

  • Nelson DL, Herbert A, Bourgoin S, Glowinski J, Hamon M (1978) Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat. Mol Pharmacol 14:983–995

    Google Scholar 

  • Palacios JM (1983) Quantitative receptor autoradiography: Application to the study of multiple sercotonin receptors in rat cortex. In: Mandel P, DeFreudis FV (eds) CNS receptors — From molecular pharmacology to behavior, Raven Press New York

    Google Scholar 

  • Peroutka SJ, Snyder SH (1979) Multiple serotonin receptors: Differential binding of (3H)-5-hydroxytryptamine, (3H)-lysergic acid diethylamide and (3H)-spiroperidol. Mol Pharmacol 16:687–699

    Google Scholar 

  • Peroutka SJ, Lebovitz RM, Snyder SH (1981) Two distinct central serotonin receptors with different physiological functions. Science 212:827–829

    Google Scholar 

  • Rainbow TC, Bleisch WV, Biegon A, McEwen BS (1982) Quantitative densitometry of neurotransmitter receptors. J Neurosci Methods 5:127–138

    Google Scholar 

  • Ramm P, Kulick JH, Stryker MP, Frost BJ (1984) Video and scanning microdensitometer-based imaging systems in autoradiographic densitometry. J Neurosci Methods 11:89–100

    Google Scholar 

  • Steinbusch HWM (1981) Distribution of serotonin-immunoreactivity in the central nervous system of the rat — Cell bodies and terminals. Neuroscience 4:557–618

    Google Scholar 

  • Steinbusch HWM, Verhofstad AAJ, Joosten HWJ (1978) Localization of serotonin in the central nervous system by immunohistochemistry: Description of a specific and sensitive technique and some applications. Neuroscience 3:811–819

    Google Scholar 

  • Unnerstall JR, Niehoff DL, Kuhar MJ, Palacios JM (1982) Quantitative receptor autoradiography using [3H]Ultrofilm: application to multiple benzodiazepine receptors. J Neurosci Methods 6:59–78

    Google Scholar 

  • Young WS, Kuhar MJ (1980) Serotonin receptor localization in rat brain by light microscopic autoradiography. Eur J Pharmacol 62:237–239

    Google Scholar 

  • Zilles K (1985) The cerebral cortex of the rat. A stereotaxic atlas. Springer, Berlin, Heidelberg, New York

    Google Scholar 

  • Zilles K, Wree A (in press) Areal and laminar structure of the rat cerebral cortex. In: G Paxinos (ed) The rat nervous system, Vol 1, Academic Press, Sydney

  • Zilles K, Zilles B, Schleicher A (1980) A quantitative approach to cytoarchitectonics. VI. The areal pattern of the cortex of the albino rat. Anat Embryol 159:335–360

    Google Scholar 

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Authors and Affiliations

  1. Anatomical Institute, University Köln, Köln, Federal Republic of Germany

    Karl Zilles & Axel Schleicher

  2. Neurobiology Department, Troponwerke, Köln, Federal Republic of Germany

    Thomas Glaser, Jörg Traber & Marion Rath

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  1. Karl Zilles
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  2. Axel Schleicher
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  3. Thomas Glaser
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  4. Jörg Traber
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  5. Marion Rath
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Zilles, K., Schleicher, A., Glaser, T. et al. The ontogenetic development of serotonin (5-HT1) receptors in various cortical regions of the rat brain. Anat Embryol 172, 255–264 (1985). https://doi.org/10.1007/BF00318973

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