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Two - phase cultivation of insect cells for production of recombinant protein

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Biotechnology Techniques

Summary

Two-phase cultivation of insect cells was carried out to produce recombinant β-galactosidase and evaluate factors influencing high cell density and high expression. The primary factor limiting specific expression at high cell concentration was not the cell concentration but the medium condition. The addition of 4 g/L yeastolate during the growth phase increased cell density while the addition of 0.6 g/L glutamine in the production phase raised the specific expression rate. Productivity of recombinant protein was consequently improved seven-fold by this operation.

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Authors and Affiliations

  1. Department of Genetic Engineering, Sung Kyun Kwan University, 300 Chunchun-dong, 440-746, Suwon, Korea

    Sung Hwan Lee & Tai Hyun Park

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  1. Sung Hwan Lee
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  2. Tai Hyun Park
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Lee, S.H., Park, T.H. Two - phase cultivation of insect cells for production of recombinant protein. Biotechnol Tech 9, 719–724 (1995). https://doi.org/10.1007/BF00159236

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  • DOI: https://doi.org/10.1007/BF00159236

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