Summary
Using an amylose-free (amf)mutant of diploid potato (Solanum tuberosum), diploid and tetraploid clones with different genotypes at the amf-locus were produced. In order to make use of the diploid material in analytic breeding of amf-potatoes, clones were selected that produced a considerable frequencies of 2n-pollen and 2n-eggs. Successful attempts were made to select normal synaptic as well as desynaptic clones producing 2n-gametes. Based on the phenotype of starch in the microspores, tetraploid clones with nulliplex, simplex and duplex genotypes at the amf-locus were selected. Prospects of using this material in conventional as well as in analytic breeding of potato are discussed.
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References
Chase, S.S., 1963. Analytic breeding in Solanum tuberosum L. A scheme utilizing parthenotes and other diploid stocks. Can. J. Genet. Cytol. 5: 359–364.
Hermsen, J.G.Th. & J. Verdenius, 1973. Selection from Solanum tuberosum group phureja of genotypes combining high-frequency haploid induction with homozygosity for embryospot. Euphytica 22: 244–259.
Hovenkamp-Hermelink, J.H.M., E. Jacobsen, A.S. Ponstein, R.G.F. Visser, G.H. Vos-Scheperkeuter, E.W. Bijmolt, J.N. de Vries, B. Witholt & W.J. Feenstra, 1987. Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.). Theor. Appl. Genet. 75: 217–221.
Hutten, R.C.B., E.J.M.M. Scholberg & J.G.Th. Hermsen, 1990. Analysis of dihaploid production and induction ability and tetraploid x pollinator interaction (Abstracts). 11th Triennial Conf. EAPR. Edinburgh, U.K. pp. 117–118.
Jacobsen, E., 1978. Die Chromosomenverdopplung in der Züchtung diploiden Kartoffeln. Ph.D. Thesis, Friedrich Wilhelm Univ. Bonn, 159 pp.
Jacobsen, E., J.H.M. Hovenkamp-Hermelink, H.T. Krijgsheld, H. Nijdam, L.P. Pijnacker, B. Withold & W.J. Feenstra, 1989a. Phenotypic and genotypic characterization of an amylose-free starch mutant of potato. Euphytica 44: 43–48.
Jacobsen, E. & W.J. Feenstra, 1989b. The use of amylose-free mutant 86.040 in potato breeding. In: Louwes, K.M., H.A.J.M. Toussaint & L.M.W. Dellaert (Eds) Proc. Conf. Breeding Section. EUCARPIA Potato sec. 1988. Wageningen, pp. 123–127.
Jongedijk, E., 1985. The pattern of megasporogenesis and early megagametogenesis in diploid Solanum species hybrids; Its relevance to the origin of 2n-eggs and the induction of apomixis. Euphytica 34: 599–611.
Mendiburu, A.O. & S.J. Peloquin, 1976. Sexual polyploidization and depolyploidization: some terminology and definitions. Theor. Appl. Genet. 48: 137–143.
Mok, D.W.S. & S.J. Peloquin, 1975. The inheritance of three mechanisms of diplandroid (2n-pollen) formation in diploid potatoes. Heredity 35: 295–302.
Ramanna, M.S., 1979. A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding. Euphytica 28: 537–561.
Ramanna, M.S., 1983. First division restitution gametes through fertile desynaptic mutants of potato. Euphytica 32: 337–350.
Uijtewaal, B.A., D.J. Huigen & J.G.Th. Hermsen, 1987. Production of potato monohaploids (2n=x=12) through prickle pollination. Theor. Appl. Genet. 73: 751–758.
Visser, R.G.F., M. Hergersberg, F.R. van der Leij, E. Jacobsen, B. Withold & W.J. Feenstra, 1989. Molecular cloning and partial characterization of the gene for granule bound starch synthase from a wild type and an amylose-free potato (Solanum tuberosum L.). Plant Science 64: 185–192.
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Jacobsen, E., Ramanna, M.S., Huigen, D.J. et al. Introduction of an amylose-free (amf) mutant into breeding of cultivated potato, Solanum tuberosum L.. Euphytica 53, 247–253 (1991). https://doi.org/10.1007/BF00023276
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DOI: https://doi.org/10.1007/BF00023276