Abstract
The protection of soft-skinned fruits against Drosophila suzukii has relied primarily on the efficacy of a few synthetic molecules. Despite their short-term efficacy, these molecules can cause environmental pollution, unintendedly affect non-target organisms, and fail to provide sustainable control. The shortfalls of using synthetic pesticides warrant the search for alternatives, such as essential oils extracted from plants, with greater eco-friendlier properties. Here, we chemically characterized and evaluated the toxicity of the essential oil extracted from leaves of Ocotea indecora (Schott) Mez (Lauraceae) against D. suzukii via two exposure pathways (ingestion and contact). We also assessed the selectivity of the essential oil to two predatory natural enemies, Eriopis connexa and Chrysoperla externa and two pollinator bees, Apis mellifera and Partamona helleri. In addition, we conducted in silico predictions to investigate potential interactions between the major compound of the essential oil and the insects’ transient receptor potential (TRP) channels. Our chromatographic analysis revealed sesquirosefuran (87%) as the major compound. Higher toxicity to adults of D. suzukii was observed in contact exposure (LC50 = 0.43 μL mL−1) compared to ingestion (LC50 = 0.72 μL mL−1). However, the essential oil did not cause mortality to the non-target organisms tested here, even when applied at 2.20 μL mL−1. Molecular predictions demonstrated that sesquirosefuran binds more stably to the TRP channels of D. suzukii than to those expressed in beneficial arthropods. Collectively, our findings provide the initial framework for the potential use of O. indecora essential oil as a sustainable alternative for managing D. suzukii infestations.
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Acknowledgements
This study was supported by the National Council of Scientific and Technological Development (CNPq, 150977/2023-9; 304179/2021-3; 309890/2022-5, and 152366/2022-9), Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ, E-26/200.915/2022; E-26/210.353/2019), Coordination for the Improvement of Higher Education Personnel (CAPES, Finance Code 001), and the Minas Gerais State Foundation for Research Aid (FAPEMIG, APQ 03771-18).
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Pedro Toledo, Sabrina Cruz, Leandro Rocha, and Eugênio Oliveira: Conceptualization, Methodology, Validation, and Writing – Original Draft. Pedro Toledo, Sabrina Cruz, Javier Mantilla, Francisco Machado, and Ana Carolina Silva: Investigation and Analysis. Leandro Rocha and Eugênio Oliveira: Supervision, Resources, Formal Analysis, Visualization, Project Administration, Fund Acquisition, and Writing – Review & Editing.
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Toledo, P.F.S., da Cruz Araujo, S.H., Mantilla Afanador , J.G. et al. Potential of Ocotea indecora Essential Oil for Controlling Drosophila suzukii: Molecular Predictions for Toxicity and Selectivity to Beneficial Arthropods. Neotrop Entomol 53, 189–199 (2024). https://doi.org/10.1007/s13744-023-01109-2
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DOI: https://doi.org/10.1007/s13744-023-01109-2