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Hydrolysis of Orange Peel with Cellulase and Pectinase to Produce Bacterial Cellulose using Gluconacetobacter xylinus

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Abstract

Oranges (Citrus sinensis) are the world’s most processed fruit. The waste from processing is rich in soluble sugars, cellulose, hemicelluloses and pectin and therefore has potential as feedstock for bacterial cellulose (BC) production. In this study, cellulase and pectinase were used to hydrolyze orange peel in order to increase the amount of fermentable sugars. Response surface methodology was used to evaluate the effects of reaction parameters, and 80.99 g/L reducing sugar was obtained with cellulase of 1589.41 U/g, pectinase of 31.75 U/g and a reaction time of 5.28 h. Besides, the orange peel fluid and orange peel hydrolysate were used as the culture media for Gluconacetobacter xylinus during BC production. The orange peel media have no significant inhibiting effect on the fermentation activity of G. xylinus for BC production. As an acetic acid buffer was used or nitrogen source was added to the orange peel media, BC production was 4.2–6.32 times higher than that in traditional Hestrin and Schramm (HS) medium. The SEM and IR spectra showed that the BC produced was not much different than that produced in HS medium. These results demonstrate that orange peel not only can be used as a low cost feedstock to produce BC, but it also provides a solution to the waste disposal problem of the orange juice industry.

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References

  1. FAO: Citrus fruit statistics. http://www.fao.org/fileadmin/templates/est/COMM_MARKETS_MONITORING/Citrus/Documents/CITRUS_BULLETIN_2012.pdf (2012)

  2. Mamma, D., Christakopoulos, P.: Biotransformation of citrus by-products into value added products. Waste Biomass Valoriz. 5(4), 529–549 (2014)

    Article  Google Scholar 

  3. Panuccio, M., Attinà, E., Basile, C., Mallamaci, C., Muscolo, A.: Use of recalcitrant agriculture wastes to produce biogas and feasible biofertilizer. Waste Biomass Valoriz. 7(2), 267–280 (2016)

    Article  Google Scholar 

  4. Wilkins, M.R., Widmer, W.W., Grohmann, K.: Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol. Process Biochem. 42(12), 1614–1619 (2007)

    Article  Google Scholar 

  5. Aravantinos-Zafiris, G., Tzia, C., Oreopoulou, V., Thomopoulos, C.D.: Fermentation of orange processing wastes for citric acid production. J. Sci. Food Agric. 65(1), 117–120 (1994)

    Article  Google Scholar 

  6. Bibi, N., Ali, S., Tabassum, R.: Statistical optimization of pectinase biosynthesis from orange peel by Bacillus licheniformis using submerged fermentation. Waste Biomass Valoriz. 7(3), 467–481 (2016)

    Article  Google Scholar 

  7. Czaja, W.K., Young, D.J., Kawecki, M., Brown, R.M.: The future prospects of microbial cellulose in biomedical applications. Biomacromolecules 8(1), 1–12 (2007)

    Article  Google Scholar 

  8. Kurniawan, H., Lai, J.T., Wang, M.J.: Biofunctionalized bacterial cellulose membranes by cold plasmas. Cellulose 19(6), 1975–1988 (2012)

    Article  Google Scholar 

  9. Budhiono, A., Rosidi, B., Taher, H., Iguchi, M.: Kinetic aspects of bacterial cellulose formation in nata-de-coco culture system. Carbohydr. Polym. 40(2), 137–143 (1999)

    Article  Google Scholar 

  10. Kurosumi, A., Sasaki, C., Yamashita, Y., Nakamura, Y.: Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693. Carbohydr. Polym. 76(2), 333–335 (2009)

    Article  Google Scholar 

  11. Hestrin, S., Schramm, M.: Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem. J. 58(2), 345 (1954)

    Article  Google Scholar 

  12. Uraki, Y., Morito, M., Kishimoto, T., Sano, Y.: Bacterial cellulose production using monosaccharides derived from hemicelluloses in water-soluble fraction of waste liquor from atmospheric acetic acid pulping. Holzforschung 56(4), 341–347 (2002)

    Article  Google Scholar 

  13. Hong, F., Qiu, K.: An alternative carbon source from konjac powder for enhancing production of bacterial cellulose in static cultures by a model strain Acetobacter aceti subsp. xylinus ATCC 23770. Carbohydr. Polym. 72(3), 545–549 (2008)

    Article  Google Scholar 

  14. Goelzer, F., Faria-Tischer, P., Vitorino, J., Sierakowski, M.R., Tischer, C.: Production and characterization of nanospheres of bacterial cellulose from Acetobacter xylinum from processed rice bark. Mater. Sci. Eng. C 29(2), 546–551 (2009)

    Article  Google Scholar 

  15. Wu, J.M., Liu, R.H.: Cost-effective production of bacterial cellulose in static cultures using distillery wastewater. J. Biosci. Bioeng. 115(3), 284–290 (2013)

    Article  Google Scholar 

  16. Grohmann, K., Cameron, R., Buslig, B.: Fractionation and pretreatment of orange peel by dilute acid hydrolysis. Bioresour. Technol. 54(2), 129–141 (1995)

    Article  Google Scholar 

  17. Kuo, C.H., Lin, P.J., Wu, Y.Q., Ye, L.Y., Yang, D.J., Shieh, C.J., Lee, C.K.: Simultaneous saccharification and fermentation of waste textiles for ethanol production. BioResources 9(2), 2866–2875 (2014)

    Article  Google Scholar 

  18. Taherzadeh, M.J., Karimi, K.: Acid-based hydrolysis processes for ethanol from lignocellulosic materials: a review. BioResources 2(3), 472–499 (2007)

    Google Scholar 

  19. Almeida, J.R., Modig, T., Petersson, A., Hähn-Hägerdal, B., Lidén, G., Gorwa-Grauslund, M.F.: Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J. Chem. Technol. Biotechnol. 82(4), 340–349 (2007)

    Article  Google Scholar 

  20. Pocan, P., Bahcegul, E., Oztop, M.H., Hamamci, H.: Enzymatic hydrolysis of fruit peels and other lignocellulosic biomass as a source of sugar. Waste Biomass Valoriz. (2017). doi:10.1007/s12649-017-9875-3

    Google Scholar 

  21. Taher, I.B., Bennour, H., Fickers, P., Hassouna, M.: Valorization of potato peels residues on cellulase production using a mixed culture of Aspergillusniger ATCC 16404 and Trichodermareesei DSMZ 970. Waste Biomass Valoriz. 8(1), 183–192 (2017)

    Article  Google Scholar 

  22. Kashyap, D., Vohra, P., Chopra, S., Tewari, R.: Applications of pectinases in the commercial sector: a review. Bioresour. Technol. 77(3), 215–227 (2001)

    Article  Google Scholar 

  23. Li, P.J., Xia, J.L., Shan, Y., Nie, Z.Y., Su, D.L., Gao, Q.R., Zhang, C., Ma, Y.L.: Optimizing production of pectinase from orange peel by Penicillium oxalicum PJ02 using response surface methodology. Waste Biomass Valoriz. 6(1), 13–22 (2015)

    Article  Google Scholar 

  24. Kuo, C.H., Chen, J.H., Liou, B.K., Lee, C.K.: Utilization of acetate buffer to improve bacterial cellulose production by Gluconacetobacter xylinus. Food Hydrocoll. 53, 98–103 (2016)

    Article  Google Scholar 

  25. Kuo, C.H., Lin, P.J., Lee, C.K.: Enzymatic saccharification of dissolution pretreated waste cellulosic fabrics for bacterial cellulose production by Gluconacetobacter xylinus. J. Chem. Technol. Biotechnol. 85(10), 1346–1352 (2010)

    Article  Google Scholar 

  26. Dien, B.S., Ximenes, E.A., O’Bryan, P.J., Moniruzzaman, M., Li, X.L., Balan, V., Dale, B., Cotta, M.A.: Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers’ grains and their conversion to ethanol. Bioresour. Technol. 99(12), 5216–5225 (2008)

    Article  Google Scholar 

  27. Zhang, M., Su, R., Qi, W., He, Z.: Enhanced enzymatic hydrolysis of lignocellulose by optimizing enzyme complexes. Appl. Biochem. Biotechnol. 160(5), 1407–1414 (2010)

    Article  Google Scholar 

  28. Zhang, J., Pakarinen, A., Viikari, L.: Synergy between cellulases and pectinases in the hydrolysis of hemp. Bioresour. Technol. 129, 302–307 (2013)

    Article  Google Scholar 

  29. Masaoka, S., Ohe, T., Sakota, N.: Production of cellulose from glucose by Acetobacter xylinum. J. Ferment. Bioeng. 75(1), 18–22 (1993)

    Article  Google Scholar 

  30. Zhong, C., Zhang, G.C., Liu, M., Zheng, X.T., Han, P.P., Jia, S.R.: Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production. Appl. Microbiol. Biotechnol. 97(14), 6189–6199 (2013)

    Article  Google Scholar 

  31. Hsieh, J.T., Wang, M.J., Lai, J.T., Liu, H.S.: A novel static cultivation of bacterial cellulose production by intermittent feeding strategy. J. Taiwan Inst. Chem. Eng. 63, 46–51 (2016)

    Article  Google Scholar 

  32. Hernández-Carranza, P., Ávila-Sosa, R., Guerrero-Beltrán, J. A., Navarro-Cruz, A. R., Corona-Jiménez, E., Ochoa-Velasco, C. E.: Optimization of antioxidant compounds extraction from fruit by-products: apple pomace, orange and banana peel. J. Food Process. Preserv. 40(1), 103–115 (2016)

    Article  Google Scholar 

  33. Keshk, S. M.: Vitamin C enhances bacterial cellulose production in Gluconacetobacter xylinus. Carbohydr. Polym. 99, 98–100 (2014)

    Article  Google Scholar 

  34. Wu, J.M., Liu, R.H.: Thin stillage supplementation greatly enhances bacterial cellulose production by Gluconacetobacter xylinus. Carbohydr. Polym. 90(1), 116–121 (2012)

    Article  Google Scholar 

  35. Shezad, O., Khan, S., Khan, T., Park, J.K.: Physicochemical and mechanical characterization of bacterial cellulose produced with an excellent productivity in static conditions using a simple fed-batch cultivation strategy. Carbohydr. Polym. 82(1), 173–180 (2010)

    Article  Google Scholar 

  36. Ul-Islam, M., Ha, J.H., Khan, T., Park, J.K.: Effects of glucuronic acid oligomers on the production, structure and properties of bacterial cellulose. Carbohydr. Polym. 92(1), 360–366 (2013)

    Article  Google Scholar 

  37. Oh, S.Y., Yoo, D.I., Shin, Y., Kim, H.C., Kim, H.Y., Chung, Y.S., Park, W.H., Youk, J.H.: Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy. Carbohydr. Res. 340(15), 2376–2391 (2005)

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by research funding grants provided by the Ministry of Science and Technology of Taiwan (MOST 104-2218-E-022-001-MY2 and 104-2221-E-005-061-MY3).

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Authors and Affiliations

  1. Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan

    Chia-Hung Kuo & Chun-Yung Huang

  2. Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

    Chwen-Jen Shieh

  3. Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan

    Hui-Min David Wang

  4. Department of Health Food, Chung Chou University of Science and Technology, Changhua, Taiwan

    Chin-Yin Tseng

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  1. Chia-Hung Kuo
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  2. Chun-Yung Huang
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Correspondence to Chia-Hung Kuo or Chwen-Jen Shieh.

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Kuo, CH., Huang, CY., Shieh, CJ. et al. Hydrolysis of Orange Peel with Cellulase and Pectinase to Produce Bacterial Cellulose using Gluconacetobacter xylinus. Waste Biomass Valor 10, 85–93 (2019). https://doi.org/10.1007/s12649-017-0034-7

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