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Biofilm and Biocontrol Modulation of Paenibacillus sp. CCB36 by Supplementation with Zinc Oxide Nanoparticles and Chitosan Nanoparticles

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Abstract

Endophytic bacteria with multi-trait plant beneficial features have applications to enhance agricultural productivity by supporting the plant growth, yield, and disease resistance. In this study, Paenibacillus sp. CCB36 was isolated from the rhizome of Curcuma caesia Roxb., and its biofilm formation and antifungal properties have been evaluated in the presence of nanoparticles. Chitosan nanoparticles (CNPs) were synthesized and characterized by UV–visible spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, high-resolution-transmission electron microscopic (HR-TEM) analysis, scanning electron microscopic (SEM) analysis, and dynamic light scattering (DLS). The effect of zinc oxide nanoparticles (ZnONPs) and CNPs on biofilm formation of Paenibacillus sp. CCB36 was evaluated by tissue culture plate assay. ZnONPs reduced its biofilm formation and was found to get modulated in the presence of CNPs as revealed by atomic force microscopy (AFM). Hence, CNPs were selected for further studies. Interestingly, biocontrol property of Paenibacillus sp. CCB36 against Rhizoctonia solani was also found to get enhanced when supplemented with chitosan nanoparticles. The results of the study indicate application of nanoparticles to improve colonization and active functioning of endophytic bacteria which can have significant application in agriculture.

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Acknowledgements

The authors acknowledge the Kerala State Council for Science, Technology and Environment (KSCSTE) under the KSCSTE-SRS, KSCSTE-KBC-YIPB, and also JAIVAM Project. The authors also acknowledge the Department of Science and Technology (DST) under DST PURSEII and Kerala State Plan Fund Project for the instrumentation facility. The authors also acknowledge the Director, Sophisticated Analytical Instrument Facility (DST-SAIF), School of Environmental Sciences, Mahatma Gandhi University, Kottayam, India, for providing atomic force microscope (AFM) facility and also acknowledge the Director, International and Interuniversity Centre for Nanoscience and Nanotechnology for the help and support for transmission electron microscopic (TEM) analysis and dynamic light scattering (DLS) facility and the Director, Center for Nanosciences, Amrita Institute of Medical Sciences, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India, for the scanning electron microscopic (SEM) analysis facility.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, India, 686 560

    Jishma Panichikkal, Ashitha Jose, Sreejith Sreekumaran & Radhakrishnan Edayileveetil Krishnankutty

  2. Department of Microbiology, St. Xavier’s College for Women, Aluva, Kerala, India, 683 101

    Anju Kanjirakandi Ashokan

  3. Department of Biosciences, M.E.S. College, Marampally (PO), Aluva, Kerala, India, 683 107

    Cimmiya Susan Baby

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  1. Jishma Panichikkal
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Contributions

Radhakrishnan Edayileveetil Krishnankutty contributed to the study conception and design. Experiments and analysis were performed by Jishma Panichikkal, Sreejith Sreekumaran, Ashitha Jose, Anju Kanjirakandi Ashokan, and Cimmiya Susan Baby. The first draft of the manuscript was written by Jishma Panichikkal. The manuscript was revised, and corrections were included by Radhakrishnan Edayileveetil Krishnankutty, Jishma Panichikkal, and Ashitha Jose. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Radhakrishnan Edayileveetil Krishnankutty.

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Panichikkal, J., Jose, A., Sreekumaran, S. et al. Biofilm and Biocontrol Modulation of Paenibacillus sp. CCB36 by Supplementation with Zinc Oxide Nanoparticles and Chitosan Nanoparticles. Appl Biochem Biotechnol 194, 1606–1620 (2022). https://doi.org/10.1007/s12010-021-03710-w

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  • DOI: https://doi.org/10.1007/s12010-021-03710-w

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