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Protective effects of resveratrol on hepatic ischemia reperfusion injury in streptozotocin-induced diabetic rats

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Abstract

Resveratrol (RSV) is a natural polyphenolic compound having antioxidant effects. This study was designed to investigate the protective effects of resveratrol against oxidative stress in hepatic ischemia–reperfusion (I/R) injury in streptozotocin (STZ)-induced diabetic rats. STZ was injected intraperitonally (i.p.) to 18 Sprague–Dawley albino rats, which were divided into three groups, each having six rats. First group was non-treated diabetic group (D), second diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period (D + I/R), and third diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period and treated with 20 mg/kg/day oral RSV before 30 min I/R injury (D + I/R + RSV). At the end of the experimental period, animals were decapitated, and blood samples were collected to determine tissue tumor necrosis factor-α (TNF-α) levels. Liver and lung tissue samples were obtained for the evaluation of biochemical parameters including malondialdehyde (MDA) and glutathione (GSH) levels and histopathological examinations. Compared to control, I/R injury resulted in decreases in GSH levels and increases in MDA levels. Tissue TNF-α levels were also increased in the D + I/R group compared to D group. Treatment with RSV prevented the alterations on biochemical parameters and histopathological changes induced by I/R. We demonstrate that in diabetic rats, hepatic I/R injury is associated with an augmented inflammatory response and oxidative stress, while RSV pre-treatment significantly decreased these responses. Larger clinical studies are desirable to determine the exact role(s) of RSV on hepatic I/R injury among diabetic subjects.

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Funding

This study was not funded from any source, except the primary institutional support.

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Authors and Affiliations

  1. Internal Medicine Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey

    Hanife Serife Aktaş

  2. General Surgery Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey

    Yahya Ozel & Ilyas Kudas

  3. AdventHealth Medical Center, 2501 N. Orange Ave., Suite 786, Orlando, FL, 32804, USA

    Sarfraz Ahmad

  4. Department of Biochemistry, Faculty of Medicine, Health Sciences University, Istanbul, Turkey

    Halime Hanim Pençe

  5. Department of Pharmacology, Pharmacy Faculty, Marmara University, Istanbul, Turkey

    Betul Ayaz-Adakul, Levent Kabasakal & Hatice Kubra Elcioglu

  6. Department of Biochemistry, Pharmacy Faculty, Marmara University, Istanbul, Turkey

    Sermin Tetik & Turgut Şekerler

  7. Department of Pathology, Prof. Dr İlhan Varank Sancaktepe Research and Training Hospital, Istanbul, Turkey

    Ceren Canbey-Göret

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  1. Hanife Serife Aktaş
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Correspondence to Hanife Serife Aktaş or Sarfraz Ahmad.

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All procedures performed in this study involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Aktaş, H.S., Ozel, Y., Ahmad, S. et al. Protective effects of resveratrol on hepatic ischemia reperfusion injury in streptozotocin-induced diabetic rats. Mol Cell Biochem 460, 217–224 (2019). https://doi.org/10.1007/s11010-019-03582-z

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  • DOI: https://doi.org/10.1007/s11010-019-03582-z

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