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The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss)

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Abstract

The study was conducted to investigate the effect of diets containing 0, 2.5, 5, 7.5, and 10% S. platensis meal on total antioxidant capacity (TAC) and lipid peroxidation, as well as the expression of two antioxidant enzyme genes (SOD and CAT) in rainbow trout (Oncorhynchus mykiss). One hundred and eighty fish, with an average initial weight of 101 ± 8 g, were cultured for 10 weeks. At the end of experiment, lipid peroxidation significantly decreased in serum of fish fed with S. platensis and fish fed with 5, 7.5, and 10% microalgae showed a significantly lower value compared to control and 2.5%. Inclusion of 7.5 and 10% S. platensis in diet also decreased lipid peroxidation in liver. TAC significantly increased with increasing level of S. platensis. Expression level of superoxide dismutase (SOD) and catalase (CAT) genes significantly increased in the fish liver after administration of microalgae and fish fed with 10% S. platensis in diet showed the highest level compared to the other treatments. The present study reveals that inclusion of 10% S. platensis in diet can decrease oxidative stress in rainbow trout. Therefore, S. platensis can be used as potential antioxidant for fish farming.

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Acknowledgements

The authors would like to thank research council of the Sari Agricultural Sciences and Natural Resources University (Project No. 08-1393-01), Sari, Iran, for supporting this research. The authors would like to acknowledge the Iranian National Elite Foundation. We are grateful to Dr. Evangeline Foronda for English editing.

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Authors and Affiliations

  1. Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, P.O. Box: 578, Sari, Iran

    Mahdi Teimouri & Sakineh Yeganeh

  2. Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Science and Natural Resources University, Sari, Iran

    Ghodrat Rahimi Mianji & Mojtaba Najafi

  3. Clinical Biochemistry, Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

    Soleiman Mahjoub

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  1. Mahdi Teimouri
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  2. Sakineh Yeganeh
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Correspondence to Mahdi Teimouri.

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Teimouri, M., Yeganeh, S., Mianji, G.R. et al. The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 45, 977–986 (2019). https://doi.org/10.1007/s10695-019-0608-3

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