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Vegetative propagation of Quercus suber L. by somatic embryogenesis

I. Factors affecting the induction in leaves from mature cork oak trees

  • Cell Biology and Morphogenesis
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Abstract

Somatic embryogenesis was induced in expanding leaves from epicormic shoots forced to sprout from segments of branches collected from several hundred-year-old cork oak trees. Following a basic protocol previously defined for leaves taken from seedlings of this species, several factors were studied to improve the response. The induction frequency was significantly higher when the length of exposure to growth regulators was increased from 7 to 30 days. The combined application of NAA and BAP was essential for induction. Although both regulators had a very significant influence, their interaction was not significant, suggesting independent roles. Leaf size had a crucial effect, because beyond a certain threshold, embryogenesis could not be obtained. Embryogenic lines were maintained via repetitive embryogenesis on hormone-free medium for more than 2 years.

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Abbreviations

BAP :

6-Benzylaminopurine

NAA :

1-Naphthaleneacetic acid

PGR :

Plant growth regulator

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Acknowledgements

The authors thank Dr. A.M. Viéitez, Prof. A. Potes and Dr. J. Alegre for their helpful comments on the manuscript. Technical assistance by Eutimio Fernández, Olga López and Nuria Cleto is gratefully acknowledged. Funds were provided by projects SC98-030 and AGL2000-0297-C03-01 and an INIA grant to I. Hernández. We wish to thank the "Servicio de Material Genético" (DGCONA Ministerio de Medio Ambiente, Madrid), and the Managing-Director and forest guard of "La Almoraima, S.A." (Cádiz) for all their help in providing plant material.

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  1. Instituto Madrileño de Investigación Agraria y Alimentaria (IMIA), Finca "El Encín", Apdo. 127, Alcalá de Henares, 28800 , Madrid, Spain

    I. Hernández, C. Celestino & M. Toribio

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  1. I. Hernández
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  2. C. Celestino
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  3. M. Toribio
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Correspondence to M. Toribio.

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Communicated by S.A. Merkle

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Hernández, I., Celestino, C. & Toribio, M. Vegetative propagation of Quercus suber L. by somatic embryogenesis. Plant Cell Rep 21, 759–764 (2003). https://doi.org/10.1007/s00299-003-0605-x

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  • DOI: https://doi.org/10.1007/s00299-003-0605-x

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