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Characterization of Arsenic-Resistant Klebsiella pneumoniae RnASA11 from Contaminated Soil and Water Samples and Its Bioremediation Potential

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Abstract

Rapid industrialization and intensive agriculture activities have led to a rise in heavy metal contamination all over the world. Chhattisgarh (India) being an industrial state, the soil and water are thickly contaminated with heavy metals, especially from arsenic (As). In the present study, we isolated 108 arsenic-resistant bacteria (both from soil and water) from different arsenic-contaminated industrial and mining sites of Chhattisgarh to explore the bacterial gene pool. Further, we screened 24 potential isolates out of 108 for their ability to tolerate a high level of arsenic. The sequencing of the 16S rRNA gene of bacterial isolates revealed that all these samples belong to different diverse genera including Bacillus, Enterobacter, Klebsiella, Pantoea, Acinetobacter, Cronobacter, Pseudomonas and Agrobacterium. The metal tolerance ability was determined by amplification of arsB (arsenite efflux gene) and arsC (arsenate reductase gene) from chromosomal DNA of isolated RnASA11, which was identified as Klebsiella pneumoniae through in silico analysis. The bacterial strains RpSWA2 and RnASA11 were found to tolerate 600 mM As (V) and 30 mM As (III) but the growth of strain RpSWA2 was slower than RnASA11. Furthermore, atomic absorption spectroscopy (AAS) of the sample obtained from bioremediation assay revealed that Klebsiella pneumoniae RnASA11 was able to reduce the arsenic concentration significantly in the presence of arsenate (44%) and arsenite (38.8%) as compared to control.

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Acknowledgements

Author RS wants to acknowledge his university, i.e. Indira Gandhi Krishi Vishwavidyalaya, Raipur, India, for financial assistance. However, this research did not receive any specific grant from funding agencies in the public, commercial, or nonprofit sectors. We also acknowledge the Department of Soil Science and Agril. Chemistry, Indira Gandhi Krishi Vishwavidyalaya, Raipur, for providing instrumentation facilities during this research.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Viswavidyalaya, Raipur, CG, India

    Prahalad Kumar, Biplab Dash, S. B. Gupta, Anup Kumar Singh, Tapas Chowdhury & Ravindra Soni

  2. Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur, Himachal Pradesh, 173101, India

    Deep Chandra Suyal

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  1. Prahalad Kumar
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Contributions

The concept, design, data analyses, manuscript preparation, and revision were performed by RS and PK. The experiments, data curation, manuscript preparation, and validation were performed by RS, PK, BD, and SBG. The project supervision, final validation, fund management, and final manuscript revision were performed by RS, SBG, and TC. All authors read and approved the manuscript.

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Correspondence to Ravindra Soni.

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This study does not describe any experimental work related to human.

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Supplementary Information

Below is the link to the electronic supplementary material.

284_2021_2602_MOESM1_ESM.jpg

Supplementary file1 Pictorial view of sample collection sites in Industrial and Mining affected area of Chhattisgarh (India). (JPG 109 kb)

284_2021_2602_MOESM2_ESM.jpg

Supplementary file2 Comparative screening of strain RpSWA2 and RnASA11. The experiment was conducted using seven treatments with three replications of each. Treatment without metal salt was treated as a positive control whereas absolute control did not contain any metal salts and bacterial culture. ( JPG 34 kb)

Supplementary file3 PCR amplification of arsB (750bp) from strain RpSWA2 and RnASA11. (JPG 25 kb)

Supplementary file4 PCR amplification of arsC (350bp) from strain RpSWA2 and RnASA11. (JPG 25 kb)

Supplementary file5 (DOCX 18 kb)

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Kumar, P., Dash, B., Suyal, D.C. et al. Characterization of Arsenic-Resistant Klebsiella pneumoniae RnASA11 from Contaminated Soil and Water Samples and Its Bioremediation Potential. Curr Microbiol 78, 3258–3267 (2021). https://doi.org/10.1007/s00284-021-02602-w

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