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Preliminary investigation of QTLs associated with seedling resistance to ascochyta blight from Cicer echinospermum, a wild relative of chickpea

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Abstract.

Accessions from Cicer echinospermum, a wild relative of chickpea (Cicer arietinum L.), contain resistance to the fungal disease ascochyta blight, a devastating disease of chickpea. A linkage map was constructed based on an interspecific F2 population, derived from a cross between a susceptible chickpea cultivar (Lasseter) and a resistant C. echinospermum accession (PI 527930). The linkage map incorporated 83 molecular markers, that included RAPD, ISSR, STMS and RGA markers; eight markers remained unlinked. The map comprised eight linkage groups and covered a map distance of 570 cM. Six out of the eight linkage groups were correlated to linkage groups from the integrated Cicer map using STMS markers. Quantitative trait loci (QTLs) associated with ascochyta blight resistance were detected using interval mapping and single-point analysis. The F2 population was evaluated for seedling and stem resistance in glasshouse trials. At least two QTLs were identified for seedling resistance, both of which were located within linkage group 4. Five markers were associated with stem resistance, four of which were also associated with seedling resistance. QTLs from previous studies also mapped to LG 4, suggesting that this linkage group is an important region of the Cicer genome for resistance to ascochyta blight.

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Acknowledgements.

B.C.Y. Collard was supported by an Australian Postgraduate Award, with the industry support of the Department of Natural Resources and Environment. We thank H. Flandez-Galvez for valuable discussion regarding the identification of QTLs associated with ascochyta blight resistance from an intraspecific chickpea population.

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Author notes

  1. B. C. Y. Collard

    Present address: Department of Biotechnology and Environmental Biology, RMIT University, PO Box 71, Bundoora, Victoria 3083, Australia,

Authors and Affiliations

  1. BioMarka, Joint Centre for Crop Innovation, Institute of Land and Food Resources, University of Melbourne, Victoria 3010, Australia

    B. C. Y. Collard & P. W. J. Taylor

  2. Department of Biotechnology and Environmental Biology, RMIT University, PO Box 71, Bundoora, Victoria 3083, Australia

    E. C. K. Pang

  3. School of Resource Management, Institute of Land and Food Resources, University of Melbourne, Victoria 3010, Australia

    P. K. Ades

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  1. B. C. Y. Collard
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Correspondence to B. C. Y. Collard.

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Communicated by P. Langridge

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Collard, B.C.Y., Pang, E.C.K., Ades, P.K. et al. Preliminary investigation of QTLs associated with seedling resistance to ascochyta blight from Cicer echinospermum, a wild relative of chickpea. Theor Appl Genet 107, 719–729 (2003). https://doi.org/10.1007/s00122-003-1297-x

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