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Microtubules and actin filaments co-localize in pollen tubes ofNicotiana tabacum L. andLilium longiflorum Thunb.

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Summary

Double labeling with fluorescent probes showed that in the cortical cytoplasm of pollen tubes ofNicotiana tabacum andLilium longiflorum, the microtubules and actin filaments co-localize for the most part. They displayed complex net-axial or helical distributions. They structural association of microtubules and actin filaments implies a functional relationship with respect to organelle movement and/or the organization of the cortical cytoplasm and cell surface.

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Abbreviations

EGTA:

ethyleneglycol-bis-(β-aminoethylether)-N,N,N,N′-tetra-acetic acid

FITC:

fluoresceine iso-thiocyanate

References

  • Cresti M, Ciampolini F, Kapil RN (1984) Generative cells of some angiosperms with particular emphasis on their microtubules. J Submicrosc Cytol 16: 317–326

    Google Scholar 

  • Derksen J, Traas JA (1984) Growth of tobacco pollen tubes in vitro; effects of drugs interfering with the cytoskeleton. In: Proc 8th int symp sexual reproduction in seeds plants, ferns and mosses, August 20–24, 1984. Wageningen, The Netherlands, pp 64–67

    Google Scholar 

  • —, Pierson ES, Traas JA (1985) Microtubules in vegetative and generative cells of pollen tubes. Eur J Cell Biol 38: 142–148

    Google Scholar 

  • Franke WW, Herth W, van der Woude WJ, Morré DJ (1972) Tubular and filamentous structures in pollen tubes: possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles. Planta 105: 317–341

    Google Scholar 

  • Hardham AR, Green PB, Lang JM (1980) Reorganization of cortical microtubules and cellulose deposition during leaf formation inGraptopetalum paraguayense. Planta 149: 181–195

    Google Scholar 

  • Heslop-Harrison J, Heslop-Harrison Y (1988) Sites of origin of the peripheral microtubule system of the vegetative cell of the angiosperm pollen tube. Ann Bot 62: 455–461

    Google Scholar 

  • — —, Cresti M, Tiezzi A, Ciampolini F (1986) Actin during pollen germination. J Cell Sci 86: 1–8

    Google Scholar 

  • Lancelle SA, Cresti M, Hepler PK (1987) Ultrastructure of the cytoskeleton in freeze-substituted pollen tubes ofNicotiana alata. Protoplasma 140: 141–150

    Google Scholar 

  • Menzel D, Schliwa M (1986 a) Motility in the siphonous green algaBryopsis. I. Spatial organization of the cytoskeleton and organelle movements. Eur J Cell Biol 40: 275–285

    Google Scholar 

  • — — (1986 b) Motility in the siphonous green algaBryopsis. II. Chloroplast movement requires organized arrays of both microtubules and actin filaments. Eur J Cell Biol 40: 286–295

    Google Scholar 

  • Perdue TD, Parthasarathy MV (1985) In situ localization of F-actin in pollen tubes. Eur J Cell Biol 39: 13–20

    Google Scholar 

  • Pierson ES (1988) Rhodamine-phalloidin staining of F-actin in pollen after dimethylsulphoxide permeabilization; a comparison with the conventional formaldehyde preparation. Sex Plant Reprod 1: 83–87

    Google Scholar 

  • —, Derksen J, Traas JA (1986) Organization of microfilaments and microtubules in pollen tubes grown in vitro or in vivo in various angiosperms. Eur J Cell Biol 41: 14–18

    Google Scholar 

  • Raudaskoski M, Aström H, Pertillä K, Virtanen I, Louhelainen J (1987) Role of the microtubule cytoskeleton in pollen tubes: an immunocytochemical and ultrastructural approach. Biol Cell 61: 177–188

    Google Scholar 

  • Seagull RW, Heath IB (1979) The effects of tannic acid on the in vivo preservation of microfilaments. Eur J Cell Biol 20: 184–188

    Google Scholar 

  • Tiwari SC, Wick SM, Williamson RE, Gunning BES (1984) Cytoskeleton and integration of cellular function in cells of higher plants. J Cell Biol 99: 63 s-69 s

    Google Scholar 

  • Traas JA, Braat P, Emons AMC, Meekes H, Derksen J (1985) Microtubules in root hairs. J Cell Sci 76: 303–320

    Google Scholar 

  • Wieland Th (1977) Modification of actins by phallotoxins. Naturwissenschaften 64: 303–309

    Google Scholar 

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Pierson, E.S., Kengen, H.M.P. & Derksen, J. Microtubules and actin filaments co-localize in pollen tubes ofNicotiana tabacum L. andLilium longiflorum Thunb.. Protoplasma 150, 75–77 (1989). https://doi.org/10.1007/BF01352923

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  • DOI: https://doi.org/10.1007/BF01352923

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