Skip to main content
SpringerLink
Log in

Ergosterol content of Rhizopus oligosporus NRRL 5905 grown in liquid and solid substrates

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The ergosterol content of Rhizopus oligosporus NRRL 5905 varied between 2–24 μg/ mg biomass dry matter when grown in laboratory media and was found to be influenced by the substrate composition.

When grown on a natural substrate (soya beans) the ergosterol content was considerably higher (estimated at approx. 60–90 μg/mg biomass dry matter).

In laboratory media, the ergosterol content was also influenced by the extent of aeration and the growth phase of the mycelium; within the range of 25° C–35° C, the incubation temperature did not influence the ergosterol content significantly.

In view of these variations, ergosterol should not be used as a chemical index for the quantification of biomass grown in static solid-substrate fermentations with limited mass transfer, e.g. tempe or oncom.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arima K, Uozumi T (1967) A new method for the estimation of the mycelial weight in koji. Agric Biol Chem 31:119–123

    Google Scholar 

  • Cannel E, Moo-Young M (1980a) Solid-state fermentation systems. Process biochem 15:2, 4 6–7

    Google Scholar 

  • Cannel E, Moo-Young M (1980b) Solid-state fermentation systems. Process Biochem 15:24–28

    Google Scholar 

  • Charles M, Gavin JR (1977) Engineering studies of the tempe fermentation. In: Steinkraus KH (ed) Handbook of indigenous fermented foods. Marcel Dekker, New York, pp 74–87

    Google Scholar 

  • Cousin MA, Zeidler CS, Nelson PE (1984) Chemical detection of mold in processed foods. J Food Sci 49:439–445

    Google Scholar 

  • Delaveau J, Lipus G, Moll M (1983) Importance des stérols au cours du cycle de la levure de brasserie. Bios 14:37–40

    Google Scholar 

  • Finger SM, Hatch RT, Regan TM (1976) Aerobic microbial growth in semisolid matrices: heat and mass transfer limitation. Biotechnol Bioeng 18:1193–1218

    Google Scholar 

  • Harris CM, Kell DB (1985) The estimation of microbial biomass. Biosensors 1:17–84

    Google Scholar 

  • Jarvis B, Seiler DAL, Ould AJL, Williams AP (1983) Observations on the enumeration of moulds in food and feeding-stuffs. J Appl Bact 55:325–336

    Google Scholar 

  • Ko SD, Hesseltine CW (1979) Tempe and related foods. In: Rose AH (ed) Economic Microbiology, Vol. 4. Microbial Biomass. Academic Press, London, pp 115–140

    Google Scholar 

  • Kronenberg H-J, Hang YD (1985) A puncture test method for monitoring solid substrate fermentation. J Food Sci 50:539–540

    Google Scholar 

  • Lindenfelser LA, Ciegler A (1975) Solid-substrate fermentor for ochratoxin A production. Appl Microbiol 29:323–327

    Google Scholar 

  • Matcham SE, Jordan BR, Wood DA (1985) Estimation of fungal biomass in a soild substrate by three independent methods. Appl Microbiol Biotechnol 21:108–112

    Google Scholar 

  • Nout MJR (1984) Influence of sample size and analytical procedure on the variance of surface mould plate counts of maizekernels. Chem Mikrobiol Technol Lebensm 8:133–136

    Google Scholar 

  • Rathbun BL, Shuler ML (1983) Heat and mass transfer effects in static solid-substrate fermentations: design of fermentation chambers. Biotechnol Bioeng 25:929–938

    Google Scholar 

  • Sakaguchi K, Okazaki H, Takeuchi M (1955) A note on the comparison of koji and submerged cultures. J Agr Chem Soc Japan 29:349

    Google Scholar 

  • Schipper MAA, Stalpers JA (1984) A revision of the genus Rhizopus. 2. The Rhizopus microsporus group. Studies in Mycology (Baarn) No. 25:20–34

  • Seitz LM, Mohr HE, Burroughs R, Sauer DB (1977) Ergosterol as an indicator of fungal invasion in grains. Cereal Chem 54:1207–1217

    CAS  Google Scholar 

  • Seitz LM, Sauer DB, Burroughs R, Mohr HE, Hubbard JD (1979) Ergosterol as a measure of fungal growth. Phytopath 69:1202–1203

    Google Scholar 

  • Seitz LM, Pomeranz Y (1983) Ergosterol, ergosta-4,6,8(14),22-tetraen-3-one, ergosterol peroxide, and chitin in ergoty barley, rye and other grasses. J Agric Food Chem 31:1036–1038

    Google Scholar 

  • Weete JD, Lawler GC, Laseter JL (1973) Total lipid and sterol components of Rhizopus arrhizus: identification and metabolism. Arch Biochem Biophys 155:411–419

    Google Scholar 

  • Yamamoto K (1957) Studies on koji. 1. A new analytical method of koji. Bull Agr Chem Soc Japan 21:308–312

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. DMV Campina, Veghel, The Netherlands

    P. G. de Koster

  2. Department of Food Science, Agricultural University, De Dreijen 12, 6703 BC, Wageningen, The Netherlands

    M. J. R. Nout, T. M. G. Bonants-van Laarhoven & P. de Jongh

Authors
  1. M. J. R. Nout
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. M. G. Bonants-van Laarhoven
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. de Jongh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. G. de Koster
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nout, M.J.R., Bonants-van Laarhoven, T.M.G., de Jongh, P. et al. Ergosterol content of Rhizopus oligosporus NRRL 5905 grown in liquid and solid substrates. Appl Microbiol Biotechnol 26, 456–461 (1987). https://doi.org/10.1007/BF00253532

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00253532

Keywords

Search

Navigation