Abstract
One hundred and forty durum wheat genotypes were used in this study. They were divided into four groups: I- mutant lines (50) II- local varieties (20), III- ACSAD lines (22) and IV-ICARDA lines (48). The genotypes were studied for the composition of high (HMWGS) and low (LMWGS) molecular weight glutenin subunits as determined by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE). All studied genotypes possessed the null allele at Glu-A1 The results demonstrated that durum wheats differed in the allelic compositions at Glu-B1. Four different allelic subunit combinations were realized at Glu-B1 with 7+8 being the most frequent and 6+15 the least frequent subunit combinations. 7+8 were abundant in groups I and III whereas subunit 20 was most frequent in groups II and IV. At Glu-B3 locus, LMW1 constituted 41% and LMW2 appeared in 59% of the genotypes. However, LMW1 was the sole allele in group I (100%) as compared to LMW2 which constituted 85%, 90% and 94% of Glu-B3 in groups II, III and IV respectively. These results differed from those reported in the literature and were discussed in terms of their potential use in breeding programs.
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Abbreviations
- HMW-GS:
-
High Molecular Weight Glutenin Subunits
- LMW-GS:
-
Low Molecular Weight Glutenin Subunits, SDS-PAGE Sodium Dodecyl Sulphate- Poly Acrylamide Gel Electrophoresis
- ACSAD:
-
Arab Center for the Studies of Arid and Dry lands
- ICARDA:
-
International Center for Research in Dry Areas
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Mir Ali, N., Arabi, M.I.E. & Al-Safadi, B. Frequencies of High and Low Molecular Weight Glutenin Subunits in Durum Wheat Grown in Syria. CEREAL RESEARCH COMMUNICATIONS 27, 301–305 (1999). https://doi.org/10.1007/BF03543540
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DOI: https://doi.org/10.1007/BF03543540