Summary
The seed proteins of ‘Chinese Spring’ wheat stocks which possess single chromosomes from other plant species related to wheat have been separated by gel electrophoresis in the presence of sodium dodecyl sulphate. Marker protein bands have been detected for both arms of barley chromosome 5, chromosome E (= 1R) and B (= 2R) of rye, chromosomes A,B (= 1Cu) and C (= 5Cu) of Aegilops umbellulata and chromosomes I and III of Agropyron elongatum. These studies, and previous findings, indicate that chromosome 5 of barley, chromosome 1R of rye, chromosome I of Ag. elongatum and possibly chromosome 1Cu of Ae. umbellulata are similar to chromosomes 1A, 1B and 1D in hexaploid wheat in that they carry genes controlling prolamins on their short arms and genes controlling high-molecular-weight (apparent molecular weight greater than 86,000) seed protein species on their long arms. These findings support the idea that all these chromosomes are derived from a common ancestral chromosome and that they have maintained their integrity since their derivation from that ancestral chromosome.
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Literature
Athwal, R.S.; Kimber, G. (1972): The pairing of an alien chromosome with homoeologous chromosomes of wheat. Can. J. Genet. Cytol. 14, 325–333
Bietz, J.A.; Wall, J.S. (1972): Wheat gluten subunits; molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cereal Chem. 49, 416–430
Bietz, J.A.; Shepherd, K.W.; Wall, J.S. (1975): Single-kernel analy-sis of glutenin: use in wheat genetics and breeding. Cereal Chem. 52, 513–532
Brown, J.W.S.; Kemble, R.J.; Law, C.N.; Flavell, R.B. (1979): Control of endosperm proteins in Triticum aestivum (var. ‘Chinese Spring’) and Aegilops umbellulata by homoeologous group 1 chromosomes. Genetics 93, 189–200
Chapman, V.; Riley, R. (1970): Homoeologous meiotic chromosome pairing in Triticum aestivum in which chromosome 5B is replaced by an alien homoeologue. Nature 226, 376–377
Driscoll, C.J.; Sears, E.R. (1971): Individual addition of the chromosomes of ‘Imperial’ rye to wheat. Agron. Absts. 1971, 6
Dunker, A.K.; Rueckert, R.R. (1969): Observations on molecular weight determinations on polyacrylamide gel. J. Biol. Chem. 244, 5074–5080
Dvorak, J.; Knott, D.R. (1974): Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to Triticum aestivum. Can. J. Genet. Cytol. 16, 399–417
Dvorak, J.; Sosulski, F.W. (1974): Effects of additions and substitutions of Agropyron elongatum chromosomes on quantitative characters in wheat. Can. J. Genet. Cytol. 16, 627–637
Hart, G.E. (1979): Genetical and chromosomal relationships among the wheats and their relatives. Stadler Symp. Univ. of Missouri 11, 9–29
Islam, A.K.M.R. (1980): Identification of wheat-barley addition lines with N banding of chromosomes. Chromosoma 76, 365–373
Islam, A.K.M.R.; Shepherd, K.W.; Sparrow, D.H.B. (1978): Production and characterization of wheat-barley addition lines. New Delhi: Proc. 5th Int. Wheat Genet. Symp., pp. 365–371
Joppa, L.R.; Bietz, J.A.; Williams, N.D. (1978): The aneuploids of durum wheat: D-genome addition and substitution lines. New Delhi: Proc. 5th Int. Wheat Genet. Symp., pp. 420–426
Kimber, G. (1967): The addition of the chromosomes of Aegilops umbellulata to Triticum aestivum (var. ‘Chinese Spring’). Genet. Res. 9, 111–114
Lawrence, G.J.; Shepherd, K.W. (1980): Variation in glutenin protein subunits of wheat. Austr. J. Biol. Sci. 33, 221–233
Osborne, T.B. (1907): The proteins of the wheat kernel. Carnegie Inst. Washington Publ. 84, 1–119
Osborne, T.B. (1924): The Vegetable Proteins. London: Longmans Green and Co.
Sears, E.R. (1968): Relationships of chromosomes 2A, 2B and 2D with their rye homoeologue. Canberra: Proc. 3rd Int. Wheat Genet. Symp., Australian Academy of Science, pp. 163–171
Sears, E.R. (1974): The wheats and their relatives. In: Handb. of Genetics Vol. 2 (ed. King, R.C.), pp. 59–91. New York-London: Plenum Press
Shepherd, K.W. (1968): Chromosomal control of endosperm proteins in wheat and rye. Canberra: Proc. 3rd Int. Wheat Genet. Symp. Australian Academy of Science, pp. 86–96
Shepherd, K.W. (1973): Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. Columbia, Missouri: Proc. 4th Int. Wheat Genet. Symp. pp. 745–760
Shepherd, K.W.; Jennings, A.C. (1971): Genetic control of rye endosperm proteins. Experientia 27, 98–99
Shewry, P.R.; Faulks, A.J.; Pickering, R.A.; Jones, I.T.; Finch, R.A.; Miflin, B.J. (1980): The genetic analysis of barley storage proteins. Heredity 44, 383–389
Weber, K.; Osborn, M. (1969): The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J.Biol. Chem. 244, 4406–4412
Wrigley, C.W.; Shepherd, K.W. (1973): Electrofocusing of grain proteins from wheat genotypes. Ann N.Y. Acad. Sci. 209, 154–162
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Lawrence, G.J., Shepherd, K.W. Chromosomal location of genes controlling seed proteins in species related to wheat. Theoret. Appl. Genetics 59, 25–31 (1981). https://doi.org/10.1007/BF00275771
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DOI: https://doi.org/10.1007/BF00275771