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Circadian temporal organization of lipidic fractions in elderly people. Entrainment to the dietary schedule

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Abstract

Background and aims: Changes in some rhythmometric parameters have been reported in the elderly as a consequence of both structural and neurochemical changes occurring in the central nervous system. Since alterations of lipid and lipoprotein metabolism are directly involved in several age-related disorders, the aim of this study was to investigate the circadian temporal organization of some important lipidic fractions (total cholesterol, triacylglycerol, apolipoprotein A1 and B) in physiological aging. Methods: Thirty old hospitalized subjects were synchronized for daily activities, sleeping/waking habits, and time/quality of meals. Twenty-four healthy young individuals served as controls. After an overnight fast, samples were taken beginning at 08:00 every 4 hours until 20:00, and every 2 hours from 20:00 to 04:00. Rhythmometric data were analyzed by single and population mean Cosinor analysis, and by ANOVA; the comparison of the rhythm’s parameters between elderly and young subjects was carried out by the Mesor test and the amplitude-acrophase using Hotelling’s test. Results: Elderly subjects exhibited statistically significant circadian rhythms for total cholesterol (p<0.00002), triacylglycerol (p<0.000001), apo A-1 (p<0.0013), and apo B (p<0.0104). Young subjects also exhibited statistically significant daily fluctuations for total cholesterol (p<0.0003), triacylglycerol (p<0.03), apo A-1 (p<0.002) and apo B (p<0.003). The mean level of apo B rhythm was higher in old subjects than in controls. Conclusions: These data suggest that the circadian temporal organization of lipidic fractions is maintained in physiological aging and underline the importance of the feeding schedule as a powerful synchronizer of the daily lipidic profile.

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References

  1. Lamon-Fava S, Jenner JL, Jacques PF, Schaefer EJ. Effects of dietary intakes on plasma lipids, lipoproteins and apolipoproteins in free-living elderly men and women. Am J Clin Nutr 1994; 59: 32–41.

    PubMed  CAS  Google Scholar 

  2. Morgan DB. The impact of ageing — present and future. Ann Clin Biochem 1983; 20: 257–61.

    PubMed  Google Scholar 

  3. Kanabrocki EL, Sothem RB, Sheving LE, et al. Reference values for circadian rhythms of 98 variables in clinically healthy men in the fifth decade of life. Chronobiol Int 1990; 7: 445–61.

    Article  PubMed  CAS  Google Scholar 

  4. Pepe G, Di Perna V, Resta F, et al. In search of a biological pattern for human longevity: impact of apo A-IV genetic polymorphism on lipoproteins and the hyper-Lp(a) in centenarians. Atherosclerosis 1998; 137: 407–17.

    Article  PubMed  CAS  Google Scholar 

  5. De Benedictis G, Franceschi C. The genetics of successful aging. Aging Clin Exp Res 1998; 10: 147–8.

    Google Scholar 

  6. Costongs GM, Janson PC, Bas BM, Hermans J, van Wersch JW, Brombacher PJ. Short-term and long-term intraindividual variations and critical differences of clinical chemical laboratory parameters. J Clin Chem Clin Biochem 1985; 23: 7–16.

    PubMed  CAS  Google Scholar 

  7. Mjos OD, Rao SN, Bjoru L, et al. A longitudinal study of the biological variability of plasma lipoproteins in healthy young adults. Atherosclerosis 1979; 34: 75–81.

    Article  PubMed  CAS  Google Scholar 

  8. Haus E, Nicolau GY, Lakatua D, Sackett-Lundeen L. Reference values for chronopharmacology. Ann Rev Chronopharmacol 1988; 4: 333–424.

    CAS  Google Scholar 

  9. Casale G, Butté M, Pasotti C, Ravecca D, De Nicola P. Antithrombin III and circadian rhythms in the aged and in myocardial infarction. Haematologica 1983; 68: 615–9.

    PubMed  CAS  Google Scholar 

  10. Casale G, Macelloni R. Circadian rhythms of lipidic fractions in elderly persons. Z Alternsforsch 1985; 40: 309–11.

    CAS  Google Scholar 

  11. Ferrari E, Cravello L, Muzzoni B, et al. Age-related changes ofthe hypothalamic-pituitary-adrenal axis: pathophysiological correlates. Eur J Endocrinol 2001; 144: 319–29.

    Article  PubMed  CAS  Google Scholar 

  12. Kruger A. The limits of normality in elderly patients. In Hamblin TJ, Ed. Balliere’s Clinical Haematology. International practice and research, Vol. 1. London: Balliere Tindall, 1987: 271–89.

    Google Scholar 

  13. Tietz NW, Shuey DF, Wekstein DR. Laboratory values in fit aging individuals. Sexagenarians through centenarians. Clin Chem 1992; 38: 1167–85.

    CAS  Google Scholar 

  14. Anesi A, Rondanelli M, Trotti R, Melzi d’Eril GV. Biological variability of myoglobin in healthy elderly and younger subjects. Aging Clin Exp Res 2000; 12: 168–72.

    CAS  Google Scholar 

  15. Leroux G, Lemieux I, Lamarche B, et al. Influence of triglycéride concentration on the relationship between lipoprotein cholesterol and apolipoprotein B and A-1 levels. Metabolism 2000; 49: 53–61.

    Article  PubMed  CAS  Google Scholar 

  16. Havel RJ. Postprandial hyperlipidemia and remnant lipoproteins. Curr Opin Lipidol 1994; 5: 102–9.

    Article  PubMed  CAS  Google Scholar 

  17. Brown SA, Boerwinkle E, Kashanian FK, Swanson N, Patsch W. Variation in concentrations of lipids, lipoprotein lipids, and apolipoproteins A-1 and B in plasma from healthy women. Clin Chem 1990; 36: 207–10.

    PubMed  CAS  Google Scholar 

  18. Frank PG, Marcel YL. Apolipoprotein A-I: structure-function relationships. J Lipid Res 2000; 41: 853–72.

    PubMed  CAS  Google Scholar 

  19. Ligthart GJ, Corberand JX, Fournier C, Galanaud P, Hijmans W, Kennes B. Admission criteria for immunogerontological studies in man: the SENIEUR protocol. Mech Ageing Dev 1984; 28: 47–55.

    Article  PubMed  CAS  Google Scholar 

  20. Chumlea WC, Steinbough ML, Roche AF, Mukherjee D, Gopalaswamy N. Nutritional anthropométric assessment in elderly persons 65 to 90 years of age. J Nutr Elderly 1985; 4: 39–51.

    Article  Google Scholar 

  21. Yesavage JA, Brink TL, Rose TL, et al. Development and validation of a geriatric depression screening scale: A preliminary report. J Psychiatr Res 1982–1983; 17: 37–49.

  22. Brown SL, Salive ME, Harris TB, Simonsick EM, Guralnik JM, Kohout FJ. Low cholesterol concentrations and severe depressive symptoms in elderly people. BMJ 1994; 308: 1328–32.

    Article  PubMed  CAS  Google Scholar 

  23. Krieger DT. Endocrine Rhythms. New York: Raven Press, 1979.

    Google Scholar 

  24. Miller M, Bachorik PS, Cloey TA. Normal variation of plasma lipoproteins: postural effects on plasma concentrations of lipids, lipoproteins, and apolipoproteins. Clin Chem 1992; 38: 569–74.

    PubMed  CAS  Google Scholar 

  25. Payne RB. Circadian rhythmic variations in serum concentrations of clinically important lipids. Clin Chem 1995; 41: 120.

    PubMed  CAS  Google Scholar 

  26. Halberg F. Chronobiology. Annu Rev Physiol 1969; 31: 675–725.

    Article  PubMed  CAS  Google Scholar 

  27. Nelson W, Tong YL, Lee JK, Halberg F. Methods for cosinor rhythmometry. Chronobiologia 1979; 6: 305–23.

    PubMed  CAS  Google Scholar 

  28. Bingham C, Arbogast B, Guillaume GC, Lee JK, Halberg F. Inferential statistical methods for estimating and comparing cosinor parameters. Chronobiologia 1982; 9: 397–439.

    PubMed  CAS  Google Scholar 

  29. Touitou Y, Haus E. Alterations with aging of the endocrine and neuroendocrine circadian system in humans. Chronobiol Int 2000; 17: 369–90.

    Article  PubMed  CAS  Google Scholar 

  30. Ravaglia G, Forti P, Maioli F, et al. Blood micronutrient and thyroid hormone concentrations in the oldest-old. J Clin Endocrinol Metab 2000; 85: 2260–5.

    Article  PubMed  CAS  Google Scholar 

  31. Kochman E, Kwarecki K. Circadian rhythms of glucose, triiodothyronine and insulin in relation to composition of diet and meal-timing in rats. Acta Physiol Pol 1984; 35: 265–75.

    PubMed  CAS  Google Scholar 

  32. Gutierrez J, Carrillo M, Zanuy S, Planas J. Daily rhythms of insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding. Gen Comp Endocrinol 1984; 55: 393–7.

    Article  PubMed  CAS  Google Scholar 

  33. Schoeller DA, Cella LK, Sinha MK, Caro JF. Entrainment of the diurnal rhythm of plasma leptin to meal timing. J Clin Invest 1997; 100: 1882–7.

    Article  PubMed  CAS  Google Scholar 

  34. Cella LK, Van Cauter E, Schoeller DA. Effect of meal timing on diurnal rhythm of human cholesterol synthesis. Am J Physiol 1995; 269: E878–83.

    PubMed  CAS  Google Scholar 

  35. Neves MQ, Carvalho MD, Passarelli M, Nakandakare ER, Bernik MM, Quintao EC. The age-related rise of plasma cholesterol in humans is not caused by a cell removal defect of LDL particles: “in vitro” studies in peripheral mononuclear blood cells. Gerontology 1997; 43: 232–41.

    Article  PubMed  CAS  Google Scholar 

  36. Barbagallo CM, Averna MR, Frada G, Chessari S, Mangiacavallo G, Notarbartolo A. Plasma lipid, apolipoprotein and Lp(a) levels in elderly normolipidemic women: relationships with coronary heart disease and longevity. Gerontology 1995; 41: 260–6.

    Article  PubMed  CAS  Google Scholar 

  37. Miller NE. Why does plasma low density lipoprotein concentration in adults increase with age? Lancet 1984; 1: 263–7.

    Article  PubMed  CAS  Google Scholar 

  38. Ericsson S, Berglund L, Frostegard J, Einarsson K, Angelin B. The influence of age on low density lipoprotein metabolism: effects of cholestyramine treatment in young and old healthy male subjects. J Intern Med 1997; 242: 329–37.

    Article  PubMed  CAS  Google Scholar 

  39. Millar JS, Lichtenstein AH, Cuchel M, et al. Impact of age on the metabolism of VLDL, IDL, and LDL apolipoprotein B-100 in men. J Lipid Res 1995; 36: 1155–67.

    PubMed  CAS  Google Scholar 

  40. Schmuck A, Fuller CJ, Devaraj S, Jialal I. Effect of aging on susceptibility of low-density lipoproteins to oxidation. Clin Chem 1995; 41: 1628–32.

    PubMed  CAS  Google Scholar 

  41. Fuentes-Arderiu X, Rivera-Coll A. Impact of circadian rhythms on the interpretation of stat measurements. Clin Chim Acta 1993; 214: 113–8.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biochemical Chemistry, Neurological Institute “C. Mondino” I.R.C.C.S., University of Pavia, Italy

    Rosita Trotti M.D.

  2. Chair of Gerontology and Geriatrics, Department of Internal Medicine, University of Pavia, Pavia

    Mariangela Rondanelli, Giovanni Cuzzoni & Ettore Ferrari

  3. Laboratory of Biochemical Chemistry, Ospedale di Circolo, University of Insubria, Varese, Italy

    Gianvico Melzi d’Eril

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  1. Rosita Trotti M.D.
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  2. Mariangela Rondanelli
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  3. Giovanni Cuzzoni
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  4. Ettore Ferrari
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  5. Gianvico Melzi d’Eril
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Correspondence to Rosita Trotti M.D..

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Trotti, R., Rondanelli, M., Cuzzoni, G. et al. Circadian temporal organization of lipidic fractions in elderly people. Entrainment to the dietary schedule. Aging Clin Exp Res 14, 94–99 (2002). https://doi.org/10.1007/BF03324422

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  • DOI: https://doi.org/10.1007/BF03324422

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