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Highly Efficient and Rapid Plant Regeneration in Citrus sinensis

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Abstract

Sweet orange (Citrus sinensis L Osbeck, var Nagpur) was explored for efficient multiple shoot regeneration and rooting in different media. The influence of phytohormones and carbon source on the in vitro morphogenesis of sweet orange epicotyl explants was investigated. Among the various concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP, Kn, Zn, and TDZ) tried, MT (Murashige and Tucker) medium fortified with benzylaminopurine (BAP) at 1 mg l−1 without auxin had a strong promotive effect on shoot regeneration, and elucidated best morphogenic response from one-month-old etiolated epicotyl explants. A 100% regeneration frequency was obtained, and multiple shoots with an average of 8.24 shoots per explant were produced on all of the explants. Root formation was seen in response to all the three auxins viz. IBA, NAA and IAA, but the best response with rapid induction was observed under the influence of indole butyric acid (IBA) at 1 mg l−1. Sucrose was observed to be at par with maltose as carbon source to support shoot regeneration. This study provided promising results, holds potential to be routinely employed for in vitro regeneration of important cultivars of Citrus spp, and can be incorporated for genetic transformation studies in citrus.

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Abbreviations

SAP:

benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

kinetin

MT:

Murashige and Tucker medium

NAA:

naphthalene acetic acid

TDZ:

thidiazurone

Zn:

zeatin

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Authors and Affiliations

  1. Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Sandeepa Singh & Manchikatla Venkat Rajam

Authors
  1. Sandeepa Singh
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  2. Manchikatla Venkat Rajam
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Correspondence to Manchikatla Venkat Rajam.

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Singh, S., Rajam, M.V. Highly Efficient and Rapid Plant Regeneration in Citrus sinensis . J. Plant Biochem. Biotechnol. 19, 195–202 (2010). https://doi.org/10.1007/BF03263340

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  • DOI: https://doi.org/10.1007/BF03263340

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