Skip to main content
SpringerLink
Log in

Molecular Cloning, Sequencing and Phylogenetic Analysis of Coat Protein Gene of a Biologically Distinct Citrus Tristeza Virus Isolate Occurring in Central India

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Citrus tristeza virus (CTV) culture, collected from a fifteen-year-old wilted and declined mosambi sweet orange [Citrus sinensis (L) Osb] plant, was maintained under green house into acid lime [C. aurantifolia Swing] and mosambi seedlings. It gave positive reaction in ELISA, both with CTV specific polyclonal antibodies (G604) and monoclonal antibody MCA13, which specifically detects severe CTV strains. Coat protein gene (CPG) of the virus was amplified by RT PCR using CPG specific primers yielding an amplicon of 672 bp. Sequence analysis of the CPG amplicon showed 97% nucleotide sequence homology with Chinese isolate CTV-0002 (Acc. No. AJ518841) and isolate S4 (Acc. No. EF063109). In phylogenetic analysis, the present CTV isolate was displayed in different clade than other reported Indian CTV isolates, but it shared a separate clade with isolates from China, Israel, Jordan and New Zealand.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bar-Joseph M, Marcus R & Lee RF, Annu Rev Phytopath, 127 (1989) 291.

    Article  Google Scholar 

  2. Ahlawat Y S, Ind J Agril Sci, 67 (1997) 51.

    Google Scholar 

  3. Permar TA, Garnsey SM, Gumpf DJ & Lee RF, Phytopathology, 80 (1990) 224.

    Article  Google Scholar 

  4. Karasev AN, Boyko VP, Gowda S, Nikolaev ON, Hilf ME, Koonin EN, Niblett CL, Cline K, Gumpf D, Lee RF, Garnsey SM, Lewandowski DJ & Dawson WO, Virology, 208 (1995) 511.

    Article  PubMed  CAS  Google Scholar 

  5. Pappu HR, Pappu SS, Niblett CL, Lee RF & Civerolo E, Virus Genes, 7 (1993) 255.

    Article  PubMed  CAS  Google Scholar 

  6. Gillings M, Civerolo EL, Gumpf DJ, Yokomi RK & Lee RF, J Virol Methods, 44 (1993) 305.

    Article  PubMed  CAS  Google Scholar 

  7. Mawassi ME, Gafny R & Bar-Joseph M, Virus Genes, 7 (1993) 265.

    Article  PubMed  CAS  Google Scholar 

  8. Bar-Joseph M, Garnsey SM, Gonsalves D, Moscovitz M & Purciful DE, Phytopathology, 69 (1979) 190.

    Article  Google Scholar 

  9. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F & Higgins DG, Nucleic Acids Res, 24 (1997) 4876.

    Article  Google Scholar 

  10. Nicholas KB & Nicholas HB Jr (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. www.psc.edu/biomed/genedoc.

    Google Scholar 

  11. Sekiya ME, Lawrence SD, McCaffery M & Cline K, J Gen Virol, 72 (1991)1013.

    Article  PubMed  CAS  Google Scholar 

  12. Manjunath KL, Pappu HR, Lee RF, Niblett CL & Civerolo EL, Proc 12 th Conf Intern Organ Citrus Virol, (1993) p 20.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Research Center for Citrus, Amravati Road, Nagpur, 440 010, Maharashtra, India

    D. K. Ghosh & Balaji Aglave

  2. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    A. Roy & Y. S. Ahlawat

Authors
  1. D. K. Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Balaji Aglave
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Roy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. S. Ahlawat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. K. Ghosh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ghosh, D.K., Aglave, B., Roy, A. et al. Molecular Cloning, Sequencing and Phylogenetic Analysis of Coat Protein Gene of a Biologically Distinct Citrus Tristeza Virus Isolate Occurring in Central India. J. Plant Biochem. Biotechnol. 18, 105–108 (2009). https://doi.org/10.1007/BF03263305

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263305

Key words

Search

Navigation