Abstract
The genome of citrus tristeza closterovirus (CTV) consists of a 20 kb single-stranded RNA encapsidated in a 2000 nm long, flexuous particle. Double-stranded (replicative form) RNAs purified from CTV-infected tissue were used to prepare complementary DNA libraries that involved initial first-strand cDNA synthesis followed by selective amplification of the coat protein gene. CTV-specific antisera were used to select clones expressing the coat protein. The coat protein genes of seven Florida and four exotic isolates that differ in their biological properties were cloned and sequenced. The gene is 669 base pairs long and encodes a 223 amino acid protein. There was a greater than 80% homology at both nucleotide and amino acid levels among all the isolates examined. However, comparisons showed that each isolate was found to have several unique amino acid residues. Several blocks of amino acid residues were conserved among all the isolates. A cluster dendrogram showed greater similarities among groups of mild and severe Florida isolates that differed significantly from those of the geographically distinct, exotic CTV isolates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bar-Joseph M. and Lee, R.F., Commonwealth Mycological Institute/Association of Applied Biologists: Description of Plant Viruses. No. 353, 1990.
Bar-Joseph M., Garnsey S.M., and Gonsalves D., Adv Virus Res25 93–168, 1979.
Bar-Joseph M., Marcus R., and Lee R.F., Annu Rev Phytopathol27 292–316, 1989.
Dodds J.A., Jordan R.L., Roistacher C.N., and Jarupat T., Intervirology27 177–188, 1987.
Valverde R.A., Dodds J.A., and Heick J.A., Phytopathology76 459–465, 1986.
Lee R.F., Proc Fla State Hort Soc97 53–56, 1984.
Yokomi R.K. and Garnsey S.M., Phytophylactica19 169–172, 1987.
Lee R.F., Brlansky R.H., Garnsey S.M., and Yokomi R.K., Phytophylactica19 215–218, 1987.
Broadbent P., Bevington K.B., and Coot B.G., Proc. 11 Conf. Intl. Organ. Citrus Virol., Riverside, CA, 1991, pp. 64–70.
Beachy R.N., Loesch-Fries S., and Tumer N.E., Annu Rev Phytopathol28 451–474, 1990.
Dulieu P. and Bar-Joseph M., J Gen Virol71 443–447, 1990.
Niblett C.L., Stark D.M., Lee R.F., and Beachy R.N., Phytopathology81 698, 1991.
Sekiya M.E., Lawrence S.D., McCaffery M., and Cline K., J Gen Virol72 1013–1020, 1991.
Pappu H.R., Lee R.F., Pappu S.S., Niblett C.L., and Karkashian J., Proc. Australian Plant Pathol. Soc., p. 23, 1991.
Rosner A., Lee R.F., and Bar-Joseph M., Phytopathology76 820–824, 1986.
Yokomi R.K., Garnsey S.M., Permar T.A., Lee R.F., and Youtsey C.O. in Proc. 11 Conf. Int. Organ. Citrus Virologists, Riverside, CA, pp. 86–92, 1991.
Rocha-Pena M.A., Lee R.F., Permar T.A., Yokomi R.K., and Garnsey S.M. in Proc. 11 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 93–102, 1991.
Van Vuuren S.P. and Moll J.N., Phytophylactica19 219–221, 1987.
Ballester-Olmos J.F., Pina J.A., and Navarro L. in Proc. 10 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 28–32, 1988.
Kano T., Garnsey S.M., Koizumi M., and Permar T.A. in Proc. 11 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 51–55, 1991.
Morris T.J. and Dodds J.A., Phytopathology69 854–858, 1979.
Sambrook J., Fritsch E.F., and Maniatis T.,Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY, 1989.
Vieira J. and Messing J., Meth Enzymol153 3–11, 1989.
Still P.E., Hunter T.J., Rocha-Pena M.A., Lee R.F., and Niblett C.L., Phytopathology81 695, 1991.
Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res12 387–395, 1984.
Rhoads D.D. and Roufa D.S., Mol Cell Biol5 1655–1659, 1985.
Garnsey S.M., Gumpf D.J., Roistacher C.N., Civerolo E.L., Lee R.F., Yokomi R.K., and Bar-Joseph M., Phytophylactica19 151–157, 1987.
Permar T.A., Garnsey S.M., Gumpf D.J., and Lee R.F., Phytopathology80 224–228, 1990.
Garnsey S.M., Kano T., Permar T.A., Cambra M., Koizumi M., and Vela C., Phytopathology79 1174, 1989.
Dawson W.O., Virology186 359–367, 1992.
Garnsey S.M., Civerolo E.L., Gumpf D.J., Yokomi R.K., and Lee R.F. in Proc. 11th Intl. Org. Citrus Virol., Riverside, CA, 1991, pp. 113–120.
Needleman S.B. and Wunsch C.D., J Mol Biol48 443–453, 1970.
Author information
Authors and Affiliations
Corresponding author
Additional information
Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of the products that may also be suitable.
Rights and permissions
About this article
Cite this article
Pappu, H., Pappu, S., Niblett, C. et al. Comparative sequence analysis of the coat proteins of biologically distinct citrus tristeza closterovirus isolates. Virus Genes 7, 255–264 (1993). https://doi.org/10.1007/BF01702586
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01702586