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Housekeeping Gene Selection for Real Time-PCR Normalization in Female Hop (Humulus lupulus L) Tissues

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Abstract

The variability of transcript accumulation of six genes encoding Chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3), DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.

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Abbreviations

Chla/b:

Chloropy1l-a/b

NADH:

Nicotinamide Adenine Dinucleotide Hydride

DRH1:

DEAD-box RNA Helicase 1

GAPDH:

Glyceraldehyde-3-Phosphate Dehydrogenase

H3:

Histone 3

7SL-RNA:

7SL component of the signal recognition particle

HEN1:

Hua Enhancer 1

Ct-value:

the point at which the measured fluorescence of PCR products crosses a threshold value

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Authors and Affiliations

  1. Institute for Agricultural and Fisheries Research, Unit Plant, Growth and Development, Caritasstraat 21, 9090, Melle, Belgium

    Lina Maloukh, Erik Van Bockstaele & Isabel Roldán-Ruiz

  2. Faculty of Bioscience Engineering, Department of Plant Production, G Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Lina Maloukh & Erik Van Bockstaele

  3. Biology Centre of the ASCR, v.v.i. Institute of Plant Molecular Biology, Branišovská 31, 370 05, Èeské Budìjovice, Czech Republic

    Jaroslav Matousek

  4. Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 37005, Èeské Budìjovice, Czech Republic

    Jaroslav Matousek

Authors
  1. Lina Maloukh
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  2. Jaroslav Matousek
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  3. Erik Van Bockstaele
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  4. Isabel Roldán-Ruiz
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Correspondence to Isabel Roldán-Ruiz.

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Maloukh, L., Matousek, J., Van Bockstaele, E. et al. Housekeeping Gene Selection for Real Time-PCR Normalization in Female Hop (Humulus lupulus L) Tissues. J. Plant Biochem. Biotechnol. 18, 53–58 (2009). https://doi.org/10.1007/BF03263295

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  • DOI: https://doi.org/10.1007/BF03263295

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