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Regeneration of Heracleum candicans Wall Plants from Callus Cultures through Organogenesis

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Abstract

Callus was initiated from petiole explants of Heracleum candicans on MS medium fortified with BAP and 2,4-D ( 0.5 mg I-1 each). Maximum shoot differentiation from callus occurred on MS medium containing 1 mg I-1 BAP and 0.2 mg I-1 NAA. The regenerated shoots were rooted on MS medium supplemented with 1 mg I-1 IBA. The rooted plants were transferred to the field after successful hardening in pots containing vermiculite. All regenerated plants were diploid with 2n=22 chromosomes in their root tip cells.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

Kinetin

NAA:

α-naphthalene acetic acid

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu, 180 006, India

    R. K. Sharma & A. K. Wakhlu

Authors
  1. R. K. Sharma
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  2. A. K. Wakhlu
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Sharma, R.K., Wakhlu, A.K. Regeneration of Heracleum candicans Wall Plants from Callus Cultures through Organogenesis. J. Plant Biochem. Biotechnol. 12, 71–72 (2003). https://doi.org/10.1007/BF03263164

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  • DOI: https://doi.org/10.1007/BF03263164

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