Abstract
Lipase fromCandida rugosa was chemically modified by amidination with imidoester hydrochlorides of different hydrophobicity. The modified enzyme showed a higher ester synthesis activity but a lower ester hydrolysis activity compared with the native enzyme. The maximum specific activity of the modified enzyme depended on its degree of derivatization. Benzene was found to be the best solvent for the synthesis reaction. The optimal temperature for the reaction was not affected by modification of the lipase. The modified lipase was more thermostable and solvent-stable than the native enzyme. When fatty acids of different carbon chainlength were tested as substrates in the synthesis of esters with the modified lipase, the highest activity was observed with myristic acid and propanol.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Inada, Y., H. Nishimura, K. Takahashi, T. Yoshimoto, A.R. Saha and Y. Saito,Biochem. Biophys. Res. Comm. 122:845 (1984).
Kaimal, T.N.B., and M. Saroja,Biotechnol. Lett. 11(1):31 (1989).
Ampon, K., A.B. Salleh, F. Salam, W.M.Z. Wan Yunus, C.N.A. Razak and M. Basri,Enz. Microb. Tech. 13:597 (1991).
Torchilin, V.P., V.G. Omel’yanenko, A.M. Klibanov, A.I. Mikhailov, V.I. Gol’danskii and V.N. Smirnov,Biochim. Biophys. Acta 602:511 (1980).
Ampon, K., and G. Means,Biotechnol. Bioeng. 32:689 (1988).
Hunter, M.J., and M.L. Ludwig,J. Am. Chem. Soc. 84:3491 (1962).
Makoff, A.J., and A.D.B. Malcolm,Biochem. J. 193:245 (1980).
Basri, M., A.B. Salleh, K. Ampon, W.M.Z. Wan Yunus and C.N.A. Razak,Biocatalysis 4:313 (1991).
Wofsy, L., and S.J. Singer,Biochem. 21:104 (1963).
Hazra, A.K., S.P. Chock and R.W. Albers,Anal. Biochem. 137:437 (1984).
Fields, R.,Biochem. J. 124:581 (1972).
Kawase, M., and A. Tanaka,Enzyme Microb. Technol. 11:44 (1989).
Laane, C., S. Boeren, K. Vos and C. Veeger,Biotechnol. Bioeng. 30:81 (1987).
Volkin, D.B., A. Staubli, R. Langer and A.M. Klibanov, Ibid.:843 (1991).
Okumura, S., M. Iwai and Y. Tsijisaka,Biochim. Biophys. Acta 575:156 (1979).
Burfield, D.R., G. Gan and R.H. Smithers,J. Appl. Chem. Biotechnol. 28:23 (1978).
Author information
Authors and Affiliations
About this article
Cite this article
Basri, M., Ampon, K., Yunus, W.M.Z. et al. Amidination of lipase with hydrophobic imidoesters. J Am Oil Chem Soc 69, 579–583 (1992). https://doi.org/10.1007/BF02636112
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02636112