Skip to main content
SpringerLink
Log in

Analysis of Genetic Diversity in Cicer arietinum L Using Random Amplified Polymorphic DNA Markers

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

PCR-based random amplified polymorphic DNA (RAPD) markers were employed to assess genetic diversity in 23 chickpea genotypes. Forty of the 100 random primers screened revealed polymorphism among the genotypes. Most of the primers revealed single polymorphic band, and only 14.1 2% of the products were polymorphic. Estimates of genetic similarity based on Jaccard’s coefficient ranged from 0.92 to 0.99, indicating narrow genetic variability among the genotypes based on RAPD markers.The 23 chickpea genotypes formed two major clusters in the dendrogram.The low RAPD polymorphism among chickpea genotypes suggests that more number of polymorphic primers need to be analysed to determine genetic relationships. It was observed that RAPD analysis employing 30 polymorphic primers could provide better estimates of genetic relationships in chickpea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Singh KB & Ocampo D, Theor Appl Genet, 95 (1997) 418.

    Article  Google Scholar 

  2. Chalmers KJ, Waugh R, Sprent JI, Simons AJ & Powell R, Heredity, 69 (1992) 465.

    Article  PubMed  Google Scholar 

  3. Gaur PM & Slinkard AE, Theor Appl Genet, 80 (1990) 648.

    Article  CAS  Google Scholar 

  4. Ahmad F, Gaur PM & Slinkard AE, Theor Appl Genet, 83 (1992) 620.

    Article  Google Scholar 

  5. Labdi M, Robertson LD, Singh KB & Charrier A, Euphyfica, 88 (1996) 181.

    Article  CAS  Google Scholar 

  6. Tayyar RI & Waines JG, Theor Appl Genet, 92 (1996) 245.

    Article  Google Scholar 

  7. Udupa SM, Sharma A, Sharma RP & Pai RA, J Plant Biochem Biotech, 2 (1993) 83.

    CAS  Google Scholar 

  8. Van Rheenen HA, Pundir RPS & Miranda JH, Curr Sci, 65 (1993) 414.

    Google Scholar 

  9. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SV, Nucleic Acids Res, 18 (1990) 6531.

    Article  PubMed  CAS  Google Scholar 

  10. Devos KM & Gale MD, Theor Appl Genet, 84 (1992) 567.

    Article  Google Scholar 

  11. Martin GB, Williams JGK & Tanksely SD, Proc Natl Acad Sci, USA, 88 (1991) 2336.

    Article  Google Scholar 

  12. Rafalski JA & Tingey SV, Trends Genet, 9 (1993) 275.

    Article  PubMed  CAS  Google Scholar 

  13. Saghai-marrof MA, Soliman KM, Jorgensen RA & Allard RW, Proc Natl Acad Sci, USA, 81 (1984) 8814.

    Google Scholar 

  14. Jaccard P, Bull Soc Vaud Sci Nat, 44 (1908) 223.

    Google Scholar 

  15. Rohlf FJ, NTSYS-pc Numerical taxonomy and multivariate analysis system. V. 1.70., Exeter Software, Setauket, NY (1993).

    Google Scholar 

  16. Mantel N, Cancer Res, 27 (1967) 209.

    PubMed  CAS  Google Scholar 

  17. Prevost A & Wilkinson MJ, Theor Appl Genet, 98 (1999) 107.

    Article  CAS  Google Scholar 

  18. Sant VJ, Patankar AG, Sarode ND, Mhase LB, Sainani MN, Deshmukh RB, Ranjekar PK & Gupta VS, Theor Appl Genet, 98 (1999) 1217.

    Article  CAS  Google Scholar 

  19. Sonnante G, Marangi A, Venora G & Pignone D, J Genet Breed, 51 (1997) 303.

    CAS  Google Scholar 

  20. Van Rheenen HA, Int Chickpea Newsl, 26 (1992) 14.

    Google Scholar 

  21. Ahmad F, Theor Appl Genet, 98 (1999) 657.

    Article  CAS  Google Scholar 

  22. Sun GL, Diaz O, Salomon B & Von Bothmer R, Genome, 42 (1999) 420.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110 012, India

    Rakesh Singh, C. Durga Prasad, Vibha Singhal & Gurinder Jit Randhawa

Authors
  1. Rakesh Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Durga Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vibha Singhal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gurinder Jit Randhawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gurinder Jit Randhawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, R., Durga Prasad, C., Singhal, V. et al. Analysis of Genetic Diversity in Cicer arietinum L Using Random Amplified Polymorphic DNA Markers. J. Plant Biochem. Biotechnol. 11, 109–112 (2002). https://doi.org/10.1007/BF03263145

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263145

Keywords

Search

Navigation