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Localization of annexin V in rat normal kidney and experimental glomerulonephritis

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Research In Experimental Medicine

Abstract

The localization of annexin V, a calcium binding protein, was immunochemically and immunohistologically studied in experimental rat glomerulonephritis using annexin V polyclonal antibody. Plasma and urinary annexin V levels were measured by a sandwich enzyme-linked immunosorbent assay (ELISA). Urinary annexin V level, which was correlated with urinary l-lactate dehydrogenase activity, N-acetyl-β-d-glucosaminidase activity and protein level, increased time-dependently after the injection of nephritogenic antigen (bovine glomerular basement membrane), progressively increasing to attain a peak level at 4 weeks of 51.5±11.3 ng/h. However, plasma annexin V level showed no increase during the study period. Normal kidneys showed strong staining for annexin V in distal tubules, being particularly strong in tubules of the inner stripe of the outer medulla, but could not be detected in proximal tubules. Annexin V was seen in visceral epithelial cells, Bowman’s capsule of the glomerulus, the vascular endothelium of arterioles and interlobular arteries, and vascular smooth muscle. In nephritis, the lumen of distal tubules and the luminal cell membrane were deeply stained, with leakage of annexin V being observed from tubular cells. In the present study, renal annexin V was markedly excreted into urine, and its urinary level reflected the severity of damage of renal tissue and the progression of nephritis. These changes of annexin V in the distal tubule and visceral epithelial cells may be of significance in cell injury of the kidney.

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Authors and Affiliations

  1. Department of Cardiology and Pneumology, Dokkyo University School of Medicine, 880 Kitakobayashi, 321-0293, Mibu, Tochigi, Japan

    Ryuko Matsuda, Noboru Kaneko, Yoshifumi Horikawa, Fumiko Chiwaki & Makoto Shinozaki

  2. Department of Clinical Laboratory Medicine, Dokkyo University School of Medicine, 880 Kitakobayashi, 321-0293, Mibu, Tochigi, Japan

    Ryuko Matsuda & Tamio Ieiri

  3. Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1 Murasaki-cho, 569-1125, Takatsuki, Osaka, Japan

    Tarou Suzuki & Nobuya Ogawa

Authors
  1. Ryuko Matsuda
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  2. Noboru Kaneko
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  3. Yoshifumi Horikawa
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  4. Fumiko Chiwaki
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  5. Makoto Shinozaki
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  6. Tamio Ieiri
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  7. Tarou Suzuki
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  8. Nobuya Ogawa
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Correspondence to Ryuko Matsuda.

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Matsuda, R., Kaneko, N., Horikawa, Y. et al. Localization of annexin V in rat normal kidney and experimental glomerulonephritis. Res Exp Med 200, 77–92 (2001). https://doi.org/10.1007/BF03220017

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  • DOI: https://doi.org/10.1007/BF03220017

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