Abstract
In living mouse kidneys, distributions of soluble proteins are usually difficult to analyze using conventional tissue preparation methods. However, “in vivo cryotechnique” (IVCT) followed by freeze-substitution is useful to examine immunolocalizations of the serum proteins in kidneys of bovine serum albumin (BSA)-overloaded mice. Following 2 days of daily intraperitoneal injection of BSA, proteinuria was evident, and BSA along with endogenous mouse albumin could be immunohistochemically detected in Bowman’s space as well as urinary tubules of adult mouse kidneys with IVCT. Immunostaining for BSA and mouse albumin was detected in Bowman’s space and proximal tubules of all nephrons, although IgG1 was not detected in about 45 % of nephrons. Immunolocalization of BSA and IgG1 observed with IVCT was different from those observed with the conventional preparation methods, which lack normal blood circulation of kidneys prior to fixation. The immunolocalization of BSA and mouse serum proteins observed with IVCT suggests that glomerular filtration barriers in living mice are functionally impaired in a manner dependent on each nephron at the early stages of the BSA-overload mouse model.
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Ohno, N., Zhou, D., Terada, N., Ohno, S. (2016). Immunohistochemical Analyses on Serum Proteins in Nephrons of Protein-Overload Mice. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_23
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DOI: https://doi.org/10.1007/978-4-431-55723-4_23
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