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Biosynthesis, isolation, purification and seperation of Zearalenone, deoxynivalenol and 15-Acetyldeoxynivalenol

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Abstract

Fusarium graminearum KF-376 isolate was found to be able to form simultaneously three toxic metabolites: zearalenone (FF-2), deoxynivalenol (DON) and 15-acetyldeoxynivalenol (15-AcDON). Toxins were extracted with methanol — water 3:1 (v/v) and purified by liquid chromatography on charcoal — Kieselgel 60 column (preliminary) and Aluminiumoxid 90 column. Final separation of the metabolites was achived on Kieselgel 60 — Aluminiumoxid 90 column.

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Authors and Affiliations

  1. Department of Chemistry, Agricultural University of Poznań, ul. Wojska Polskiego 75, 60-625, Poznań, Poland

    M Kostecki & P Goliński

  2. Department of Plant Pathology, Agricultural University of Warsaw, ul. Nowoursynowska 166, 02-766, Warsaw, Poland

    J Chełkowski

Authors
  1. M Kostecki
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  2. P Goliński
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  3. J Chełkowski
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Kostecki, M., Goliński, P. & Chełkowski, J. Biosynthesis, isolation, purification and seperation of Zearalenone, deoxynivalenol and 15-Acetyldeoxynivalenol. Mycotox Res 7 (Suppl 2), 156–159 (1991). https://doi.org/10.1007/BF03192202

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  • DOI: https://doi.org/10.1007/BF03192202

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