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Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay

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Abstract

From a single aflatoxin B1 oxime — bovine serum albumin conjugate, polyclonal and monoclonal antibody preparations were produced. The four rabbit polyclonal antisera were specific for aflatoxin Bi in a microtitration plate enzyme — linked immunosorbent assay. The monoclonal antibodies showed a wide range of differing specificities, recognizing, for example, aflatoxins B1, B2, G1 and G2; B1 and B2; B1 and G1; and G1 alone. No antibody preparations reacted with aflatoxin M1. The significance of these results to the strategy of anti-aflatoxin antibody production for use in quantitative enzyme immunoassays is discussed.

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References

  1. Van Rensburg SJ, Cook-Mozaffari P, Van Schalkewyk DJ, Van der Watt JJ, Vincent TJ, Purchase IF (1985) Hepatocellular carcinoma and dietary aflatoxins in Mozambique and Transkei. Br J Cancer 51:713–726

    PubMed  Google Scholar 

  2. IARC (1987) Aflatoxins.In: IARC Monographs on the evaluation of the carcinogenic risk of chemicals to humans, International Agency for Research on Cancer, Lyon, Suppl 7, pp 83–87

  3. Shepherd MJ, Mortimer DN, Gilbert J (1987) A review of approaches to the rapid analysis of aflatoxins in food. J Assoc Public Anal 25:129–149

    CAS  Google Scholar 

  4. Morgan MRA (1989) Mycotoxin immunoassay (with special reference to ELISAs). Tetrahedron 45:2237–2249

    Article  CAS  Google Scholar 

  5. Landsteiner K (1945) The specificity of serological reactions. Harvard University Press, Boston, USA

    Google Scholar 

  6. Chu FS (1986) Immunoassays for mycotoxins.In: Cole RJ (Ed) Modern methods in the analysis and structural elucidation of mycotoxins. Academic Press, pp 207–237

  7. Langone JJ, Van Vunakis J (1976) Aflatoxin B1, specific antibodies and their use in radioimmunoassay. J nat Cancer Inst 56:591–595

    CAS  PubMed  Google Scholar 

  8. Morgan MRA, Kang AS, Chan HW-S (1986) Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay. J Sci Fd Agric 37:908–914

    Article  CAS  Google Scholar 

  9. Lau HP, Gaur PK, Chu FS (1981) Preparation and characterization of aflatoxin B2 — hemiglutarate and its use for the production of antibody against aflatoxin. J Food Safety 3:1–13

    Article  CAS  Google Scholar 

  10. Lawellin DW, Grant DW, Joyce BK (1977) Enzyme — linked immunosorbent analysis for aflatoxin B1. Appl Environ Microbiol 34:94–96

    CAS  PubMed  Google Scholar 

  11. Pestka JJ, Chu FS (1984) Aflatoxin B1 dihydrodiol antibody: Production and specificity. Appl Environ Microbiol 47:472–477

    CAS  PubMed  Google Scholar 

  12. Chu FS, Steinert BW, Gaur PK (1985) Production and characterisation of antibody against aflatoxin G1. J Food Safety 7:161–170

    Article  CAS  Google Scholar 

  13. Fan TS, Zhang GS, Chu FS (1984) Production and characterisation of antibody against aflatoxin Q1. Appl Environ Mitrobiol 47:526–532

    CAS  Google Scholar 

  14. Gaur PK, El-Nakib O, Chu FS (1980) Comparison of antibody production against aflatoxin B1 in goats and rabbits. Appl Environ Microbiol 40:678–680

    CAS  PubMed  Google Scholar 

  15. Candlish AAG, Stimpson WH, Smith JE (1985) A monoclonal antibody to aflatoxin B1: detection of the mycotoxin by enzyme immunoassay. Lett Appl Micro 1:57–61

    Article  CAS  Google Scholar 

  16. Kaveri SV, Fremy J-M, Lapeyre C, Strosberg AD (1987) Immunodetection and immunopurification of aflatoxins using a high affinity monoclonal antibody to aflatoxin B1. Lett Appl Micro 4:71–75

    Article  CAS  Google Scholar 

  17. Dean PDG, Exley D, Johnson MW (1971) Preparation of 17 b — oestradiol — 6-(O-carboxymethyl) oxime — bovine serum albumin conjugate. Steroids 18:593–603

    Article  CAS  PubMed  Google Scholar 

  18. Erlanger BF, Borek OF, Beiser SM, Liebermann SJ (1959) Steroid — protein conjugates II. Preparation and characterisation of conjugates of bovine serum albumin with progesterone, deoxycorticosterone and estrone. J biol Chem 234:1090–1094

    CAS  PubMed  Google Scholar 

  19. Morgan MRA, McNerney R, Mathew JA, Coxon DT, Chan HW- S (1983) An enzyme — linked immunosorbent assay for total glycoalkaloids in potato tubers. J Sci Fd Agric 34:593–598

    Article  CAS  Google Scholar 

  20. Galfrè G, Milstein C (1981) Preparation of monoclonal antibodies: Strategies and procedures:In: Langone JJ, Van Vunakis H (Eds) Methods in enzymology. Academic Press, 73:3–46

    Article  PubMed  Google Scholar 

  21. Voller A, Bidwell DE, Bartlett A (1979) The enzyme — linked immunosorbent assay (ELISA). Dynatech Europe UK.

  22. Ekins RP (1983) The precision profile: Its use in assay design, assessment and quality control.In: Immunoassays for clinical chemistry. Hunter WM, Corrie JET (Eds) Churchill Livingstone, pp 76–105

  23. Chu FS, Ueno I (1977) Production of antibody against aflatoxin B1. Appl Environ Microbiol 33:1125–1128

    CAS  PubMed  Google Scholar 

  24. Pestka JJ, Gaur PK, Chu FS (1980) Quantitation of aflatoxin B1 antibody by an enzyme — linked immunosorbent microassay. Appl Environ Microbiol 40:1027–1031

    CAS  PubMed  Google Scholar 

  25. Groopman JD, Trudel LJ, Donahue PR, Marshak-Rothstein A, Wogan GN (1984) High — affinity monoclonal antibodies for aflatoxins and their application to solid-phase immunoassays. Proc natl Acad Sei USA 81:7728–7731

    Article  CAS  Google Scholar 

  26. Dixon-Holland DE, Pestka JJ, Bidigare BA, Casale WL, Warner RL, Ram BP, Hart LP (1988) Production of sensitive monoclonal antibodies to aflatoxin M1 and their application to ELISA of naturally contaminated foods. J Food Prot 51:201–204

    CAS  Google Scholar 

  27. Chu FS, Lau HP, Fan TS, Zhang GS (1982) Ethylenediamine modified bovine serum albumin as protein carrier in the production of antibody against mycotoxins. J Immunol Methods 55:73–78

    Article  CAS  PubMed  Google Scholar 

  28. Hastings KL, Tulis JJ, Dean JH (1988) Production and characterization of a monoclonal antibody to aflatoxin B2. J Agric Food Chem 36:404–408

    Article  CAS  Google Scholar 

  29. Morgan MRA, Kang AS, Chan HW-S (1986) Production of antisera against sterigmatocystin hemiacetal and its potential for use in an enzyme — linked immunosorbent assay for sterigmatocystin in barley. J Sci Fd Agric 37:873–888

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. AFRC Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK

    CM Ward, AP Wilkinson, S Bramham, HA Lee, HW-S Chan & MRA Morgan

  2. Monoclonal Antibody Centre, Department of Immunology, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, CB2 4AT, Babraham, Cambridge, UK

    GW Butcher & A Hutchings

Authors
  1. CM Ward
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  2. AP Wilkinson
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  3. S Bramham
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  4. HA Lee
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  5. HW-S Chan
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  6. GW Butcher
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  7. A Hutchings
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  8. MRA Morgan
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Ward, C., Wilkinson, A., Bramham, S. et al. Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay. Mycotox Res 6, 73–83 (1990). https://doi.org/10.1007/BF03192146

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  • DOI: https://doi.org/10.1007/BF03192146

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