Summary
Praziquantel was determined in body fluids by gas liquid chromatography as follows:
A known amount of an internal standard and 0.1 N sodium hydroxide solution was added to the sample to be analyzed. After extraction with methyl acetate/diisopropyl ether = 30/70, the organic extract was evaporated, the residue taken up in methyl acetate and an aliquot injected for glc analysis. Separation was accomplished on a OV3 silicon oil phase, and for detection and quantitation, a thermoionic FID sensitized for nitrogen-containing compounds was used.
The determination limit in serum is about 0.01 μg/ml. The relative standard deviation for serum concentrations of 0.1 μg/ml was found to be 4.5%.
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References
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Patzschke K., Pütter J., Wegner L.A., Horster F.A., Diekmann H.W. (1979): Serum concentrations and renal excretion in humans after oral administration of Praziquantel — results of three determination methods. Europ. J. Drug Metabolism and Pharmacokinetics,4, 149–156.
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Pütter J. (1979): A fluorimetric method for the determination of Praziquantel in blood plasma and urine. Europ. J. Drug Metabolism and Pharmacokinetics,4, 143–148.
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Diekmann, H.W. Quantitative determination of praziquantel in body fluids by gas liquid chromatography. European Journal of Drug Metabolism and Pharmacokinetics 4, 139–141 (1979). https://doi.org/10.1007/BF03189416
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DOI: https://doi.org/10.1007/BF03189416