Abstract
The 14-3-3 is a highly conserved, ubiquitous expressed protein in eukaryotes and very important in the regulation of such crucial cellular processes as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, and malignant transformation. In this study, the full-length cDNA of 14-3-3 was cloned first time from a white-rot fungusPhanerochaete chrysosporium. Yeast two hybrid has been performed to fish out the proteins that can bind with 14-3-3 inP. chrysosporium. The results showed that 14-3-3 could form homodimers inP. chrysosporium. Two novel proteins containing predicted WD domain could interact with 14-3-3, too. In addition, the transcription of 14-3-3 gene under low-nitrogen medium was constitutional by RT-PCR analysis. These results indicate that 14-3-3 protein inP. chrysosporium may be involved in multiple cellular processes.
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Hu, G., Feng, H., Zhang, T. et al. Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungusPhanerochaete chrysosporium . Ann. Microbiol. 56, 191–196 (2006). https://doi.org/10.1007/BF03175004
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DOI: https://doi.org/10.1007/BF03175004