Abstract
An echo-detected electron paramagnetic resonance (ED EPR) approach was used to study molecular dynamics in photosynthetic reaction centers (RCs) fromRhodobacter sphaeroides R26, employing the specific spin label methanethiosulfonate and 3-maliemido proxyl. ED EPR has recently been shown to be sensitive to so-called dynamical transition in disordered media, which is characterized by the transition from a harmonic-like librational motion of a molecule to an anharmonic one or to a stochastic wobbling motion. ED EPR line shapes studied over a wide temperature range reveal a sharp transition occurring above 180 K. The possible relation of the found transition to the temperature dependence of electron transfer reactions in RC is discussed.
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Borovykh, I.V., Gast, P. & Dzuba, S.A. The dynamical transition in proteins of bacterial photosynthetic reaction centers observed by echo-detected EPR of specific spin labels. Appl. Magn. Reson. 31, 159–166 (2007). https://doi.org/10.1007/BF03166253
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DOI: https://doi.org/10.1007/BF03166253