Skip to main content
SpringerLink
Log in

Role of adipogenic and thermogenic genes in susceptibility or resistance to develop diet-induced obesity in rats

Papel de genes adipogénicos y termogénicos en la resistencia o susceptibilidad al desarrollo de obesidad inducida por la dieta en rata

  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The aim of the present work was to assess whether changes in adipose tissue gene expression related with adipogenesis and/or thermogenesis could be involved in the mechanism conferring susceptibility or resistance to develop obesity in high-fat fed outbreed rats. For this purpose, male Wistar rats were fed with standard laboratory diet (control group) or high fat diet. After 15 days, two groups of rats with significant differences on body weight gain in response to the high fat diet were characterized and identified as diet-induced obesity (DIO) and diet resistant (DR) rats. A significant increase in visceral white adipose tissue (WAT) PRARγ and aP2 (p<0.05) mRNA levels associated to a decrease in RARγ expression (p<0.05) was observed in DIO rats, suggesting an increase of adipogenesis. Furthermore, our data showed a marked increase in brown adipose tissue (BAT) of UCP1 mRNA in DIO animals (p<0.01) (without affecting PGC-1α gene expression), whereas no changes were found in WAT UCP2 gene expression. All these data suggest that the variations found in the expression pattern of PPARγ, aP2 and RARγ by high-fat diet could be involved, at least in part, in the differences in body weight gain and adiposity observed between DR and DIO animals. The compensatory adaptations through the increase in energy expenditure by changes on the expression levels of UCP1 seem not to be enough to avoid the obesity onset in the DIO group.

Resumen

EI objeto del presente trabajo fue determinar si cambios en la expresión de genes del tejido adiposo relacionados con la adipogénesis y la termogénesis pudieran estar implicados en la resistencia o susceptibilidad al desarrollo de obesidad en ratas alimentadas con dieta hipergrasa. Tras 15 días de ingesta de la dieta rica en grasa (dieta de cafetería), se identificó un grupo susceptible (DIO) y otro resistente (DR) al desarrollo de obesidad. El incremento significativo observado en la expresión de PPARγ y aP2 (p<0.05), junto con el descenso en la expresión de RARγ (p<0.05) en el tejido adiposo blaco de ratas DIO, sugiere un incremento del proceso adipogénico en estos animales. Además, nuestros datos mostraron aumento en los niveles de ARNm de UCP1 (sin afectar a la expresión del gen PGC-1α) en el tejido adiposo marrón en los animales DIO, mientras que no se observaron cambios en la expresión de UCP2 en tejido adiposo blanco. Todos estos datos sugieren que los cambios encontrados en la expresión de PPARγ, aP2 y RARγ podrían estar implicados en las diferencias en la ganancia del peso corporal y la adiposidad observadas entre los grupos DIO y DR. Los mecanismos compensadores puestos en marcha mediante el incremento de los niveles de expresión de UCP1 no parecen ser suficientes para evitar la instauración de la obesidad en este grupo de animales.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Archer, Z.A., Rayner, D.V., Duncan, J.S., Bell, L.M., Mercer, J.G. (2005): J. Neuroendocrinol., 17, 10–17.

    Article  CAS  PubMed  Google Scholar 

  2. Bairras, C., Menard, L., Redonnet, A., Ferrand, C., Delage, B., Noel-Suberville, C., Atgié, C., Higueret, P. (2005): J. Physiol. Biochem., 61, 353–361.

    Article  CAS  PubMed  Google Scholar 

  3. Chang, S., Graham, B., Yakubu, F., Lin, D., Peters, J.C., Hill, J.O. (1990): Am. J. Physiol., 259, 1103–1110.

    Google Scholar 

  4. Clegg, D.J., Benoit, S.C., Reed, J.A., Woods, S.C., Dunn-Meynell, A., Levin, B.E. (2005): Am. J. Physiol. Regul. Integr. Comp. Physiol., 288, 981–986.

    Google Scholar 

  5. DiRenzo, J., Soderstrom, M., Kurokawa, R., Ogliastro, M.H., Ricote, M., Ingrey, S., Hörlein, A., Rosenfeld, M.G., Glass, C.K. (1977): Mol. Cell. Biol., 17, 2166–2176.

    Google Scholar 

  6. González-Muniesa, P., Milagro, F.I., Campion, J., Martínez, J.A. (2006): Int. J. Mol. Med., 17, 591–597.

    PubMed  Google Scholar 

  7. Han, J., Hajjar, D.P., Zhou, X., Gotto, A.M., Jr., Nicholson, A.C. (2002): J. Biol. Chem., 277, 23582–23586.

    Article  CAS  PubMed  Google Scholar 

  8. Hassanain, M., Levin, B.E. (2002): Brain Res., 929, 175–180.

    Article  CAS  PubMed  Google Scholar 

  9. Hu, E., Kim, J.B., Sarraf, P., Spiegelman, B.M. (1996): Science, 274, 2100–2103.

    Article  CAS  PubMed  Google Scholar 

  10. Hunt, C.R., Ro, J.H., Dobson, D.E., Min, H.Y., Spiegelman, B.M. (1986): Proc. Natl. Acad. Sci. U S A., 83, 3786–3790.

    Article  CAS  PubMed  Google Scholar 

  11. Irani, B.G., Dunn-Meynell, A.A., Levin, B.E. (2007): Endocrinology., 148, 310–316.

    Article  CAS  PubMed  Google Scholar 

  12. Ji, H., Friedman, M.I. (2003): Physiol. Behav., 78, 767–772.

    Article  CAS  PubMed  Google Scholar 

  13. Kopecky, J., Rossmeisl, M., Flachs, P., Brauner, P., Sponarova, J., Matejkova, O., Prazák, T., Ruzicková, J., Bardová, K., Kuda, O. (2004): Physiol. Res., 53, 225–232.

    Google Scholar 

  14. Levin, B.E., Triscari, J., Sullivan, A.C. (1983): Am. J. Physiol., 245, 364–371.

    Google Scholar 

  15. Levin, B.E. (1993): Obes. Res., 1, 281–287.

    CAS  PubMed  Google Scholar 

  16. Levin, B.E., Dunn-Meynell, A.A., Balkan, B., Keesey, R.E. (1997): Am. J. Physiol., 273, 725–730.

    Google Scholar 

  17. Levin, B.E. (1999): Am. J. Physiol., 276, 382–387.

    Google Scholar 

  18. Levin, B.E. (2007): J. Physiol., DOI: 10.1113/jphysiol.2007.135434

  19. Margareto, J., Gomez-Ambrosi, J., Marti, A., Martínez, J.A. (2001a): Biochem. Biophys. Res. Commun., 283, 6–11.

    Article  CAS  PubMed  Google Scholar 

  20. Margareto, J., Larrarte, E., Marti, A., Martínez, J.A. (2001b): Biochem. Pharmacol., 61, 1471–1478.

    Article  CAS  PubMed  Google Scholar 

  21. Margareto, J., Marti, A., Martínez, J.A. (2001c): J. Nutr. Biochem., 12, 130–137.

    Article  CAS  PubMed  Google Scholar 

  22. Marrades, M.P., Milagro, F.I., Martínez, J.A., Moreno-Aliaga, M.J. (2006): Biochem. Biophys. Res. Commun., 339, 785–789.

    Article  CAS  PubMed  Google Scholar 

  23. Moreno-Aliaga, M.J., Santos, J.L., Marti, A., Martínez, J.A. (2005): Obes. Rev., 6, 155–168.

    Article  CAS  PubMed  Google Scholar 

  24. Oberkofler, H., Esterbauer, H., Linnemayr, V., Strosberg, A.D., Krempler, F., Patsch, W. (2002): J. Biol. Chem., 277, 16750–16757.

    Article  CAS  PubMed  Google Scholar 

  25. Pérez-Echarri, N., Pérez-Matute, P., Martínez, J.A., Marti, A., Moreno-Aliaga, M.J. (2005): J. Physiol. Biochem., 61, 333–342.

    Article  PubMed  Google Scholar 

  26. Pérez-Matute, P., Marti, A., Martínez, J.A., Fernández-Otero, M.P., Stanhope, K.L., Havel, P.J., Moreno-Aliaga, M.J. (2005): Am. J. Physiol. Regul. Integr. Comp. Physiol., 288, 1682–1688.

    Google Scholar 

  27. Pérez-Matute, P., Pérez-Echarri, N., Martínez, J.A., Marti, A., Moreno-Aliaga, M.J. (2007): Br. J. Nutr., 97, 389–398.

    Article  PubMed  Google Scholar 

  28. Prpic, V., Watson, P.M., Frampton, I.C., Sabol, M.A., Jezek, G.E., Gettys, T.W. (2002): J. Nutr., 132, 3325–3332.

    CAS  PubMed  Google Scholar 

  29. Rankinen, T., Zuberi, A., Chagnon, Y.C., Weisnagel, S.J., Argyropoulos, G., Walts, B., Pérusse, L., Bouchard, C. (2006): Obesity, 14, 529–644.

    Article  PubMed  Google Scholar 

  30. Redonnet, A., Groubet, R., Noel-Suberville, C., Bonilla, S., Martínez, A., Higueret, P. (2001): Metabolism, 50, 1161–1167.

    Article  CAS  PubMed  Google Scholar 

  31. Redonnet, A., Bonilla, S., Noel-Suberville, C., Pallet, V., Dabadie, H., Gin, H., Higueret, P. (2002): Int. J. Obes. Relat. Metab. Disord., 26, 920–927.

    Article  CAS  PubMed  Google Scholar 

  32. Ribot, J., Felipe, F., Bonet, M.L., Palou, A. (2001): Obes. Res., 9, 500–509.

    Article  CAS  PubMed  Google Scholar 

  33. Rosen, E.D., Hsu, C.H., Wang, X., Sakai, S., Freeman, M.W., Gonzalez, F.J., Spiegelman, B.M. (2002): Genes Dev., 6, 22–26.

    Article  Google Scholar 

  34. Scheja, L., Makowski, L., Uysal, K.T., Wiesbrock, S.M., Shimshek, D.R., Meyers, D.S., Morgan, M., Parker, R.A., Hotamisligil, G.S. (1999): Diabetes, 48, 1987–1894

    Article  CAS  PubMed  Google Scholar 

  35. Spiegelman, B.M., Puigserver, P., Wu, Z. (2000): Int. J. Obes. Relat. Metab. Disord., 4, 8–10.

    Google Scholar 

  36. Surwit, R.S., Wang, S., Petro, A.E., Sanchis, D., Raimbault, S., Ricquier, D., Collins, S. (1998): Proc. Natl. Acad. Sci. U S A., 95, 4061–4065.

    Article  CAS  PubMed  Google Scholar 

  37. Tontonoz, P., Hu, E., Graves, R.A., Budavari, A.I., Spiegelman, B.M. (1994): Genes Dev., 8, 1224–1234.

    Article  CAS  PubMed  Google Scholar 

  38. Watson, P.M., Commins, S.P., Beiler, R.J., Hatcher, H.C., Gettys, T.W. (2000): Am. J. Physiol. Endocrinol. Metab., 279, 356–365.

    Google Scholar 

  39. Xue, J.C., Schwarz, E.J., Chawla, A., Lazar, M.A. (1996): Mol. Cell. Biol., 16, 1567–1575.

    CAS  PubMed  Google Scholar 

  40. Ziotopoulou, M., Mantzoros, C.S., Hileman, S.M., Flier, J.S. Am. J. Physiol. Endocrinol. Metab., 279, 838–845.

Download references

Author information

Authors and Affiliations

  1. Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, 31008, Pamplona, Spain

    N. Pérez-Echarri, J. A. Martínez & M. J. Moreno-Aliaga

  2. Unité de Nutrition et Signalisation Cellulaire, ISTAB, Université de Bordeaux I, Talance, France

    C. Noel-Suberville, A. Redonnet & P. Higueret

Authors
  1. N. Pérez-Echarri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Noel-Suberville
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Redonnet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Higueret
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. A. Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. J. Moreno-Aliaga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. J. Moreno-Aliaga.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez-Echarri, N., Noel-Suberville, C., Redonnet, A. et al. Role of adipogenic and thermogenic genes in susceptibility or resistance to develop diet-induced obesity in rats. J. Physiol. Biochem. 63, 317–327 (2007). https://doi.org/10.1007/BF03165763

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03165763

Key words

Palabras clave

Search

Navigation