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Effects of ion channel modulators in the influx and efflux of Tc-99m-MIBI

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Abstract

Possible involvement of cell membrane ion transport systems in the uptake and extrusion of Tc-99m-MIBI was investigated by using various buffers with or without Na+ and Ca++, and ion transport inhibitors in a tumor cell line. The ion transport modulators dimethyl amiloride (DMA), verapamil, flunarizine and monensin were used. The uptake of Tc-99m-MIBI was significantly increased in all buffers containing either Na+ or Ca++ alone or none of them. There was significantly increased uptake of Tc-99m-MIBI especially in buffers without Na+. Verapamil, a L-type Ca++ channel blocker, increased Tc-99m-MIBI uptake in all buffers. Flunarizine, which inhibits Na+/ Ca++ channels, caused significantly increased accumulation of Tc-99m-MIBI only in buffer containing both Na+ and Ca++. Monensin, a sodium ionophore, significantly increased uptake of Tc-99m-MIBI. DMA, a potent Na+/H+ antiport inhibitor, significantly inhibited the uptake of Tc-99m-MIBI in all buffers. In conclusion, Tc-99m-MIBI behaves like Na+ during its uptake and extrusion. Extrusion of Tc-99m-MIBI may involve both verapamil- and flunarizine-sensitive pathways.

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Authors and Affiliations

  1. Department of Radiology, Yamanashi Medical University, Tamaho-cho, Nakakoma-gun, 409-3898, Yamanashi, Japan

    Ali S. Arbab, Keiji Toyama, Takao Arai & Tsutomu Araki

  2. Department of Radiology, Tokyo Medical College Hachioji Medical Center, Japan

    Kiyoshi Koizumi

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  1. Ali S. Arbab
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  2. Kiyoshi Koizumi
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  3. Keiji Toyama
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  5. Tsutomu Araki
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Correspondence to Ali S. Arbab.

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Arbab, A.S., Koizumi, K., Toyama, K. et al. Effects of ion channel modulators in the influx and efflux of Tc-99m-MIBI. Ann Nucl Med 13, 27–32 (1999). https://doi.org/10.1007/BF03165424

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  • DOI: https://doi.org/10.1007/BF03165424

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